O- Amino acids + Proteins Flashcards
1
Q
What are zwitterions?
A
- have a perm +ve charge & perm -ve charge
- neutral overall
2
Q
melting points + solubility of amino acids
A
- high mp
- soluble in water
- both ∵ ionic
3
Q
How long does a polypeptide chain has to be to be called a protein?
A
- > 50 a.a.
4
Q
amide functional group
A
O=C-NH2
5
Q
amide linkage/ peptide bond
A
-CONH-
6
Q
hydrolysis of protein/ peptide
A
- boiled w/ HCl
- conc = 6mol/dm3
- for ~ 24hrs
- all peptide bonds hydrolysed
7
Q
What bonds/ forces hold primary structure tgt?
A
covalent
8
Q
What bonds hold secondary structure tgt?
A
H bonds
- disrupted by gentle heat/ Δ in pH
9
Q
What bonds/ forces hold tertiary structure tgt?
A
- H bond
- ionic
- disulphide bridge
- vdW forces (in all molecules)
10
Q
Determining structure of a protein
A
- hydrolysis- reflux w/ 6mol/dm3 HCl
- a.a. separated + identified by thin-layer chromatography (TLC) {sometimes 2-dimensional TLC)
