Nutrition & Trace Elements Flashcards
In what form in iron stored in the body?
Ferritin:
24 subunit protein – MW ~450 kD
Binds up to 4500 Fe3+ as hydroxyphosphate
Present in most cells, esp. liver, spleen, marrow
Readily mobilized as needed
Hemosiderin:
Insoluble complex derived from ferritin
Decreased apoferritin content
Releases iron less readily than ferritin
Iron is part of what 5 important things in the body?
1 Hemoglobin (2500 mg)
2 Myoglobin (130 mg)
3 Enzymes, cytochromes (8 mg)
4 Transferrin, plasma (3 mg)
In a typical diet 10-20 mg/day is available yet only 5-10% of that is absorbed. Where does iron absorption take place?
Absorbed in duodenum and jejunum only as Fe2+
Gastric reducing environment (e.g., vitamin C) helpful
How is iron transported within plasma and why?
Free unbound iron is toxic. So it is bound and transported very transferrin.
Transferrin:
ß-globulin glycoprotein
MW 79.5 kD
Two Fe3+ binding sites per molecule
Trf is in excess; Fe saturation 20-50% typically
—–Specific Trf receptor for tissue uptake
How is serum iron measured? What type of specimen can be used?
Specimen: serum or heparinized plasma
Principle:
Fe3+ is released from binding proteins by acidification
Fe3+—> Fe2+ by ascorbate or other reducing agent
Fe2+ binds to ferrozine, ferene, or bathophenanthroline and is measured with a spectrophotometer
Usually not affected by hemolysis
Heme iron not released in assay
Test should also include iron-binding capacity
Reference Interval:
Male 65-170 mcg/dL
Female 50-170 mcg/dL
Diurnal variation (high in am, low in pm)
How is transferrin measured?
Immunoassay (nephelometry or turbidimetry)
Reference interval 200-320 mg/dL
What is TIBC? How is it measured?
TIBC-Total Iron Binding Capacity (semi-automated):
Add excess Fe3+ to sample and equilibrate
Complex non-bound iron (e.g., MgCO3) and separate
Measure Trf-bound iron (as in total iron)
Reference interval: 175-400 mcg/dL (iron)
What is UIBC? How is it measured?
UIBC-Unsaturated Iron Binding Capacity (automated)
Add excess Fe3+ to sample and equilibrate
Measure unbound iron only (no acidification)
TIBC = UIBC + serum iron
UIBC = Added iron-measured iron
Do not report UIBC results, only TIBC (& percent saturation)
How is Transferrin Saturation and Transferrin itself calculated?
Transferrin Saturation = Fe ÷ TIBC (or Trf)
—Usually expressed as a percent
Transferrin (mg/dL) ≈ 0.8 x TIBC (mcg Fe/dL)
TIBC reference interval: 175-400 mcg/dL
Trf Sat’n reference interval: 15-60% (TIBC)
Serum ferritin is ______ to stored iron and will _____ as iron stores______.
directly proportional.
decrease.
deplete.
What are the tree stages of iron deficiency anemia?
Stage 1 : Iron DEPLETION
Depletion of iron stores marked by ferritin decrease
Normal serum iron and TIBC (ferritin maintains serum levels)
No anemia
Normal RBC morphology
Stage 2: Iron-deficient Erythropoiesis
Decreased iron available for erythropoiesis (stores depleted)
Decreased ferritin, serum iron, Trf sat’n; increase in TIBC (Trf)
No anemia; RBC slightly microcytic, no hypochromia, inc. RDW
Stage 3 : Iron DEFICIENCY ANEMIA
Decrease in ferritin, iron, Trf sat’n; increase in TIBC (Trf)
Decrease in hemoglobin, MCV, MCH, MCHC; inc. RDW
Microcytic, hypochromic anemia
Describe the pathogensis of anemia due to chronic disease.
Inflammatory cytokines induce hepcidin production by the liver
Hepcidin prevents iron release from enterocytes and macrophages
Low available iron interferes with bacterial growth
What role does copper play in the body?
Enzyme cofactor – esp. redox enzymes
Bound to ceruloplasmin (α2-globulin)
Copper deficiency leads to…?
Deficiency:
Microcytic, hypochromic anemia
Neutropenia
Copper excess is called…?
Excess: Wilson’s disease
Copper deposition in tissues, esp. liver and brain
Decreased ceruloplasmin and serum Cu, plus elevated urine Cu
How is copper measured?
Measure Cu by atomic absorption, ceruloplasmin by immunoassay (nephelometry or turbidimetry)
What are the fat soluble vitamins?
A: carotene, retinol, retinal, retinoic acid
Vision
Epithelial cell stability
D: cholecalciferol
Ca2+ absorption
E: tocopherols
Antioxidant, free radical scavenger
K: phylloquinones, menaquinones
Synthesis of clotting factors II, VII, IX, X, C and S
Cofactor for enzymatic formation of γ-carboxyglutamate