Nukleinske kisline Flashcards

1
Q

Naštej nukleozide

A

Citidin, Uridin, Timidin, adenozin, gvanozin

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2
Q

Funkcija cAMP in cGMP

A

sekundarna sporočevalca, regulatorja metabolizma

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3
Q

Naštej 3 fiziološko aktivne derivate purinov in pirimidinov.

A

Kofein, aciklovir, 6-merkaptopurin…

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4
Q

Vezava fosfata in sladkorja?

A

Fosfor je vezan na 5´ konec, kisik pa na 3´ konec

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5
Q

Kako beremo sekvenco?

A

5->3

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6
Q

Funkcija snRNK in siRNK?

A
  1. Procesiranje mRNK v jedru

2. regulacija genske ekspresije, utišanje genov

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7
Q

Kako je stabiliziran dvojni heliks DNK v vodni raztopini?

A

Z H-vezmi, Van der Walsovimi in hidrofobnimi interakcijami.

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8
Q

Kaj omogoča razvitje dvojnega heliksa? (Hiperkromni učinek)

A

Z vezavo prot., pH, temp., denaturanti

H. u. je povečana absorpcija UV svetlobe zardi denaturacije.

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9
Q

Strukturni elementi 3D strukture RNK.

A

Lasnice, desno sučni dvojni heliksi, interne zanke in izbokline.

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10
Q

Struktura tRNK, metilirane baze, 3D struktura?

A

1.
2.
3. L oblika

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11
Q

Tip a, tip b nukleaze

A

a: cepi iz 3´ konca; b: cepi iz 5´ konca

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12
Q

Restrikcijske endonukleaze

A

Zaščita bakt. pred vdorom tuje DNK; matična zaščitena z metilacijo

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13
Q

Naštej nukleoproteine

A

virusi, ribosomi, nukleosomi, kromosomi, ribonukleoproteinski encimi

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14
Q

Pakiranje v kromosom

A

nukleosom - kromatinska ogrlica - solenoid - zanke - vrvica - kromosom

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