Nucleus Flashcards

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1
Q

What does the contents of the nucleus contain?

A

Aqueous solution
DNA
RNA
Proteins

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2
Q

What is the nuclear lamina?

A

Proteins which provide support and organisation within the nucleus

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3
Q

What are lamins?

A

The proteins which make up the lamina

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4
Q

What shape does the lamina take?

A

2D lattice of fibres

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5
Q

What is the structure of a lamin?

A

Globular head
Rod like central helix domain called a dimer
Pairs of dimers twist to form long filaments

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6
Q

How is the lamina in contact with the inner membrane?

A

Anchors at the end of lamins attach the lamina to the inner membrane

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7
Q

What is the nucleolus and what does it contain?

A

An area within the nucleus that is not encapsulated by a membrane
Contains ribosomal RNA

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8
Q

What occurs in the nucleolus?

A

Ribosomal subunit synthesis

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9
Q

What is the nuclear envelope made of?

A

An inner and outer membrane

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10
Q

What other component of the cell is the inner membrane attached to?

A

The nuclear lamina

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11
Q

What other component of the cell is the outer membrane attached to?

A

The endoplasmic reticulum

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12
Q

What molecules are transported across the nuclear envelope?

A

Nuclear proteins - in
RNA - out
Ribosomal subunits - out

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13
Q

What is the structure of the nuclear pore?

A

Cytoplasmic protrusions
Central ring
Nuclear basket structure

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14
Q

What does the nuclear basket structure do?

A

Regulates molecules being transported into and out of the nucleus

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15
Q

How do small molecules pass through nuclear pores?

A

Passive diffusion

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16
Q

How do large molecules pass through nuclear pores?

A

Active transport

17
Q

What does NLS stand for?

A

Nuclear localisation signal

18
Q

What is the NLS?

A

A positively charged sequence of amino acids within a protein that ‘tags’ it for import into the nucleus

19
Q

Describe the process of nuclear import.

A

Nuclear import receptors (importins) recognise the NLS on a molecule and bind to it
This importin NLS complex passes from one subunit to another until it enters the nucleus
Once it has entered the nucleus the complex dissociates and the importin travels back out of the nucleus to be used again whilst the molecule remains in the nucleus