nucleic acids and DNA replication Flashcards
what does DNA do
holds genetic info
what does RNA do
transfers genetic info from DNA to ribosomes
what makes up a DNA nucleotide
- deoxyribose as a pentose sugar
- nitogen containing bases ( adenine, thymine, cytosine and guanine)
- contains a phosphate group
what does a RNA nucleotide contain
- ribose as the oentose sugar
- nitrogen bases ( adenine, uracil, cytosine and guanine)
- contains a phosphate group
what is the structure of DNA
- polynucleotide
- phosphodiester bonds between adjacent nuclotides, formed by condensation reactions
- double helix with two polynucleotide chains held togather by hydrogen bonds between specific complementary base pair
what is the structure of RNA
- short polynucleotide chain
- 3 different types ;
messengar RNA ( mRNA )
transfer RNA ( tRNA )
ribosomal RNA (rRNA)
what is the structure of mRNA
- linear in shape
- no hydrogen bonds
- contains codons
what is the structure of tRNA
- clover leaf shape
- contains H bonds
- contains anticodons
- has an amino acid binding site
- transports amino acids
what is the structure of rRNA
- contains H bonds
what are the differences between DNA and mRNA
DNA ;
- deoxyribose
- thymine
- long
- double stranded
- contains H bonds
mRNA ;
- ribose
- uracil
- short
- single stranded
- does not contain H bonds
what is the structure of prokaryotic DNA ( how is it different to DNA)
- shorter
- fewer genes
- circular not linear
- not associated with histones
- no introns present
- sometimes there are additional plasmids
what is the process of semi conservative replication
1) DNA helicase is an enzyme which unwinds the DNA double helix by breaking the H bonds between complementary bases in the polynucleotide strands
2) this gives a single stranded DNA with exposed bases that act as a template determining the order of nucleotides on the newly synthesised strand.
3) new DNA nucleotides are attracted to exposed bases on template strands and form complementary base pairs
4) DNA polymerase joins adjecent nucleotides by condensation reaction forming phosphodiester bonds.
5) after replication each DNA molecule contains one original template strand and one newley synthesised strand