Nucleic acids Flashcards

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1
Q

What is the dipolar water molecule?

A

A water molecule is made up of two atoms of hydrogen and one of oxygen.
Although the molecule has no overall charge, the oxygen atom has a slight negative charge, while the hydrogen atoms have a slight positive charge.
The water molecule therefore has positive and negative poles so is dipolar.

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2
Q

How are water molecules bonded?

A

Different poles attract, and therefore the positive pole of one water molecule will be attracted to the negative pole of another molecule.
The attractive force between these opposite charges is called a hydrogen bond.
Although each bond is fairly weak, together they form important forces that cause the water molecules to stick together, giving water its unusual properties.

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3
Q

Why does water have a high specific heat capacity of water?

A

Because water molecules stick together, it takes more energy to separate them than would be needed if they did not bond together.
The boiling point of water is therefore high.
Without its hydrogen bonding, water would be a gas at the temperature commonly found on earth.
So it takes more energy to heat a given mass of water.

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4
Q

What is specific heat capacity?

A

The heat energy needed to raise the temperature of 1kg of water by 1°c.
It takes a lot of energy to raise the temperature of water.

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5
Q

Why is a high specific heat capacity of water important to living organisms?

A

Water acts as a buffer against sudden temperature variations, making the aquatic environment a temperature-stable one.
As organisms are mostly water, it also buffers them against sudden temperature changes especially in terrestrial environments.
The bodies of living organisms are mainly water, the water in and around our cells absorbs a lot of heat energy without its temperature increasing much.

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6
Q

What is the latent heat of vaporisation of water?

A

Hydrogen bonding between water molecules means that it requires a lot of energy to evaporate 1 gram of water.

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7
Q

What is the importance of large latent heat of vapourisation?

A

Animals that are able to sweat can keep cool as the water in sweat evaporates off the surface of the animal.
Plants are also cooled when water evaporates from their leaves.

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8
Q

What is cohesion and surface tension in water?

A

Cohesion of water is the tendency of molecules to stick together.
With its hydrogen bonding, water has large cohesive forces and these allow it to be pulled up through a tube, such as a xylem vessel in plants.
Surface tension is where water molecules meet air they tend to be pulled back into the body of water rather than escaping from it.

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9
Q

What is the importance of strong cohesion to water molecules?

A

In plants, water moves up the xylem vessels as a continuous stream.
This allows the water to move from the roots to the top of the tallest trees.
Water cohesion leads to surface tension.
This means water behave as if there is a skin where the water meets the air.
This allows small animals to live on the surface of water bodies, e.g. pond skaters.

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10
Q

What is the importance of water to living organisms?

A

Water is the main constituent of all organisms:
Up to 98% of a jellyfish is water and mammals are typically 65% water.
Water is also where life on earth arose and it is the environment in which many species still live.

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11
Q

How is water used in metabolism?

A

Water is used to break down many complex molecules by hydrolysis, for example proteins to amino acids.
Water is produced in condensation reactions.
Chemical reactions take place in an aqueous medium.
Water is a major raw material in photosynthesis.

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12
Q

What is the importance of water in metabolic reactions to living organisms?

A

Hydrolysis reactions are important in digestion of large molecules in animals.
Condensation reactions are important in synthesis of important molecules such as proteins in living organisms.

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13
Q

How is water used as a solvent?

A

Water readily dissolves other substances:
Gases such as oxygen and carbon dioxide.
Wastes such as ammonia and urea.
Enzymes, whose reactions take place in solutions.
Inorganic ions and small hydrophilic molecules such as amino acids, monosaccharides and ATP.

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14
Q

What is the importance of water as a solvent to living organisms?

A

The metabolic reactions that happen inside all living organisms can only happen when the reactants are dissolved in water.
Substances being dissolved in water also allows them to be transported around the bodies of living organisms.

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15
Q

What are other important features of water?

A

Its evaporation cools organisms and allows them to control their temperature.
It is not easily compressed and therefore provides support, for example the hydrostatic skeleton of animals such as the earthworm and turgor pressure in herbaceous plants.
It is transparent and therefore aquatic plants can photosynthesise and also light rays can penetrate the jelly-like fluid that fills the eye and so can reach the retina.

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16
Q

Where are inorganic ions found?

A

In organisms where they occur in solution in the cytoplasm of cells and in body fluids and as well as part of larger molecules.
They may be in concentrations that range from very high to very low.

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17
Q

What are the functions of inorganic ions?

A

The specific function a particular ion performs is related to its properties.
For example, iron ions are found in haemoglobin where they play a role in the transport of oxygen.
Other examples include the phosphate ions that form a structural role in DNA molecules and a role in storing energy in ATP molecules.
Hydrogen ions are important in determining the pH of solutions and therefore the functioning of enzymes.
Sodium ions are important in the transport of glucose and amino acids across plasma membranes.

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18
Q

What is the structure of ATP?

A

Adenosine triphosphate is a phosphorylated macromolecule with three parts:
Adenine - a nitrogen-containing organic base.
Ribose - a pentose sugar molecule that acts as the backbone to which other parts are attached.
Phosphates - a chain of three phosphate groups.

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19
Q

How does ATP store energy?

A

The bonds between the phosphate groups are unstable and so have a low activation energy, which means they are easily broken.
When they do break they release a considerable amount of energy.
Usually in living cells it is only the terminal phosphate that is removed.

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20
Q

What is the equation for converting ATP into ADP?

A

ATP + H2O = ADP + Pi + energy.
Adenosine triphosphate + water = adenosine diphosphate + inorganic phosphate + energy.
As water is used to convert ATP to ADP, it is a hydrolysis reaction.
The reaction is catalysed by the enzyme ATP hydrolase.

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21
Q

When does ATP synthesis occur?

A

The synthesis of ATP from ADP involves the addition of a phosphate molecule:
In chlorophyll-containing plant cells during photosynthesis (photophosphorylation).
In plant and animal cells during respiration (oxidative phosphorylation).
In plant and animal cells when phosphate groups are transferred from donor molecules to ADP (substrate-level phosphorylation).

22
Q

What is ATP synthesis?

A

The conversion of ATP to ADP is reversible and therefore energy can be used to add an inorganic phosphate to ADP to re-form ATP according to the reverse of the equation.
The reaction is catalysed by the enzyme ATP synthase.
Water is removed in this process so it is a condensation reaction.

23
Q

Why is ATP a better immediate energy store than glucose?

A

Each ATP molecule releases less energy than each glucose molecule. The energy for reactions is therefore released in smaller, more manageable quantities rather than the much greater, and therefore less manageable, release of energy from a glucose molecule.
The hydrolysis of ATP to ADP is a single reaction that releases immediate energy. The breakdown of glucose is a long series of reactions and therefore the energy release takes longer.

24
Q

What does the instability of the phosphate bonds in ATP mean?

A

It makes ATP a good energy donor, but it is not a long-term energy store.
Fats and carbohydrates such as glycogen, serve this purpose much better.
It is instead an immediate energy source of a cell.
As a result, cells do not store large quantities of ATP, but maintain just a few seconds supply.
This is not a problem as ATP is rapidly reformed from ADP and inorganic phosphate and so a little goes a long way.

25
Q

Why do some cells possess many large mitochondria?

A

ATP cannot be stored and so has to be continuously made within the mitochondria of cells that need it.
Cells, such as muscle fibres and the epithelium of the small intestine, which require energy for movement and active transport, possess many large mitochondria.

26
Q

Which energy requiring processes is ATP used in?

A

Metabolic processes
Movement
Active transport
Secretion
Activation of molecules
Bioluminescence

27
Q

How is ATP used in metabolic processes?

A

ATP provides the energy needed to build up macromolecules from their basic units.
For example, making starch from glucose or polypeptides from amino acids.

28
Q

How is ATP used for movement?

A

ATP provides the energy for muscle contraction.
In muscle contraction, ATP provides the energy for the filaments of muscle to slide past one another and therefore shorten the overall length of a muscle fibre.

29
Q

How is ATP used in active transport?

A

ATP provides the energy to change the shape of carrier proteins in plasma membranes.
This allows molecules or ions to be moved against a concentration gradient.

30
Q

How is ATP used for secretion?

A

ATP is needed to form the lysosomes necessary for the secretion of cell products.

31
Q

How is ATP used in the activation of molecules?

A

The inorganic phosphate released during the hydrolysis of ATP can be used to phosphorylate other compounds in order to make them more reactive, thus lowering the activation energy in enzyme-catalysed reactions.
For example, the addition of phosphate to glucose molecules at the start of glycolysis.

32
Q

What components are individual nucleotides made from?

A

A pentose sugar
A phosphate group
A nitrogen-containing organic base: Cytosine, Thymine, Uracil, Adenine and Guanine.

33
Q

How is a mononucleotide formed?

A

The condensation reactions between pentose sugar, phosphate group and organic bases.

34
Q

How is a dinucleotide formed?

A

Two mononucleotides are joined in a condensation reaction between the deoxyribose sugar of one and the phosphate group of another.
The bond formed is a phosphodiester bond.
Polynucleotides can then be formed by the continued linking of mononucleotides.

35
Q

What is the structure of ribonucleic acid (RNA)?

A

It is a polymer made up of nucleotides.
It is a single, relatively short polynucleotide chain.
The pentose sugar is always ribose.
The organic bases are always adenine, guanine, cytosine and uracil.

36
Q

What are the types of RNA?

A

tRNA transfers genetic information from DNA to the ribosomes.
mRNA is messenger RNA.
rRNA is ribosomal RNA that with proteins, makes up ribosomes.

37
Q

What is the structure of DNA?

A

The pentose sugar is deoxyribose.
The organic bases are adenine, thymine, guanine and cytosine.
DNA is made up of two strands of nucleotides (double stranded).
Each strand is extremely long, and they are joined together by hydrogen bonds formed between certain bases.

38
Q

Who discovered the structure of DNA?

A

In 1953, James Watson and Francis Crick worked out the structure of DNA, after Rosalind Franklin made pioneering work on the X-Ray diffraction patterns of DNA.

39
Q

What is the base pairing?

A

Adenine always pairs with thymine.
There are two hydrogen bonds between these.
Guanine always pairs with cytosine.
There are three hydrogen bonds between these.
These pairs are complementary.
The quantities of adenine and thymine in DNA are always the same, however the ratio of adenine and thymine to guanine and cytosine varies between species.

40
Q

What are pyrimidines and purines?

A

Pyrimidines have a big name and are a smaller molecule:
Cytosine and thymine.
Purines have a small name and are a big molecule:
Guanine and adenine.

41
Q

Why is DNA a stable molecule?

A

The phosphodiester backbone protects the more chemically reactive organic bases inside the double helix.
Hydrogen bonds link the organic base pairs forming bridges between the phosphodiester uprights.
As there are three hydrogen bonds between cytosine and guanine, the higher the proportion of C-G pairings the more stable the DNA molecule.
There are interactive forces between the base pairs that hold the molecule together.

42
Q

What is the function of DNA?

A

It is the hereditary material responsible for passing genetic material from cell to cell and generation to generation.
In total, there are around 3.2 billion base pairs on the DNA of a typical mammalian cell.
This vast number means that there is an almost infinite variety of sequences of bases along the length of a DNA molecule.
This variety provides the genetic diversity within living organisms.

43
Q

How is DNA adapted to carry out its function?

A

It is a very stable molecule which normally passes from generation to generation without change. Only rarely does it mutate.
Its two separate strands are joined only with hydrogen bonds, which allow them to separate during DNA replication and protein synthesis.
It is an extremely large molecule and therefore carries an immense amount of genetic information.
By having the base pairs within the helical cylinder of the deoxyribose-phosphate backbone, the genetic information is to some extent protected from being corrupted by outside chemical and physical forces.
Base pairing leads to DNA being able to replicate and to transfer information as mRNA.

44
Q

What is the difference in structure between DNA and RNA?

A

RNA is single stranded whereas DNA is double stranded.
RNA has the sugar ribose whereas DNA has deoxyribose.
RNA bases are ACGU, DNA is ACGT.

45
Q

What is nuclear division?

A

The process by which the nucleus divides.
There are two types, meiosis and mitosis.

46
Q

What is cytokinesis?

A

It follows nuclear division and is the process by which the whole cell divides.

47
Q

What are the requirements for semi-conservative replication to take place?

A

The four types of nucleotide, each with their bases of adenine, guanine, cytosine or thymine, must be present.
Both strands of the DNA molecule act as a template for the attachment of these nucleotides.
The enzyme polymerase.
A source of chemical energy is required to drive the process.

48
Q

What is the process of semi-conservative replication?

A

The enzyme DNA helicase breaks the hydrogen bonds linking the base pairs of DNA.
As a results the double helix separates into its two strands and unwinds.
Each exposed polynucleotide strand then acts as a template to which complementary free nucleotides bind by specific base pairing.
Nucleotides are joined together in a condensation reaction by polymerase to form the ‘missing’ polynucleotide strand on each of the two original polynucleotide strands of DNA.
Each of the new DNA molecules contains one of the original DNA strands.

49
Q

What is the conservative model?

A

It suggested that the original DNA molecule remained intact and that a separate daughter DNA copy was built up from new molecules of deoxyribose, phosphate, and organic bases.
Of the two molecules produced, one would be made of entirely new material while the other would be entirely original material.
This is wrong and was replaced by the semi-conservative model.

50
Q

What is the differences between prokaryotic DNA and eukaryotic DNA?

A

In prokaryotes, DNA is circular, whereas in eukaryotes DNA is linear.
Prokaryotic DNA is in the cytoplasm whereas it is in the nucleus.
Prokaryotes have plasmids whereas eukaryotes do not.
Prokaryotes are not associated with histone proteins where eukaryotic DNA is wound around histone proteins.
Eukaryotic DNA is longer than prokaryotic.

51
Q

What are the similarities between eukaryotic and prokaryotic DNA?

A

They both have a double helix structure.
Nucleotide structure is identical.
The nucleotides are both joined by phosphodiester bonds.