NSCs and Adult Neurogenesis Flashcards

1
Q

totipotent vs pluripotent vs multipotent

A

totipotent - generate every cell type in embryo and placenta

pluripotent - generate every cell type in embryo

multipotent - generate multiple specialised cell types in a tissue

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2
Q

population studies of stem cells

A

immunohistochemisty
single cell RNA-sequencing
thymidine analogues

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3
Q

clonal analysis

A

lineage tracing

genetic or viral mediated labelling most common

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4
Q

neurogenesis in SVZ

A

NSCs are Type B cells. They generate Type C cells which are neural progenitor cells. TC generate TA cells which are neuroblasts, that migrate through RMS to olfactory bulb

differentiate into different types of interneurons

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5
Q

neurogenesis in hippocampus

A

dentate gyrus
NSCs are radial glial-like cells, multipotent and usually quiescent
only granule cells are generated

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