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Clinical Bacteriology Lab > NonFermenting and Misellaneous Gram-Neg Bacilli > Flashcards

NonFermenting and Misellaneous Gram-Neg Bacilli Flashcards

1
Q

Most of the enteronacteriacae in this chapter are oxidase ___,
grow on ___ agar, and oxidize ___

A

oxidase positive,
grow on MacConkey agar, and oxidize glucose;

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2
Q

NONFERMENTING AND MISELLANEOUS GRAM-
NEGATIVE BACILLI
Withstand the treatments with

A

Chlorhexidine and Quartenary Ammonium Compounds

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3
Q

Agar used in biochemical test to determine fermenters and non fermenters

A

TSIA or Kligler iron agar (KIA)

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4
Q

A fermenter typically produces an ___ on TSIA or KIA within 18 hours of incubation

A

acid (yellow) butt with an acid or alkaline (red) slant

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5
Q

A nonfermenter produces __ in the butt and slant or may produce an _ slant.

A

no change in butt, alkaline (red) slant

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6
Q

Pseudomonas morphology

A

‘Gram-negative bacillus or coccopacilus

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7
Q

Pseudomonas motility

A

Motile, usually with polar flagellum or polar tuft of flagella

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8
Q

• blue phenazine pigment that contributes to the characteristic green color of P. aeruginosa, damages cells by producing reactive oxygen species.

A

Pyocyanin

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9
Q

pseudomonas are oxidase and catalase _

A

Positive

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10
Q

Pseudomonas fluorescent group, which includes P. aeruginosa, P. fluorescens, P. putida, P. veronii, P. mosselii, and P. monteilii produce

A

Pyoverdin

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11
Q

is water soluble and fluoresces under short-wavelength ultraviolet ligh

A

Pyoverdin

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12
Q

Most strains of P. aeruginosa will also produce the blue, water-soluble pigment called

A

Pyocyanin

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13
Q

other water soluble pigments occasionally produced I by strains of P. aeruginosa.

A

pyorubin (red) and pyomelanin (brown or black).

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14
Q

Other key characteristics of P. aeruginosa include :
- denitrification of _
-positive for _
- growth at _
- _ positivity
- _ utilization

A
  • denitrification of nitrates to nitrite
    -positive for r arginine dihydrolase (ADH)
  • growth at 42°C,
  • citrate positivity
  • acetamide utilization
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15
Q

is a selective and differential medium for the identification of P. aeruginosa.

A

Cetrimide agar

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16
Q

acts as a detergent and inhibits most bacteria; the medium also enhances the production of the two pigments produced by P. aeruginosa.

A

Cetrimide

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17
Q

No other nonfermentative, gram-negative
bacillus produces __, so its presence can be used to specifically identify P. aeruginosa

A

Pyocyanin

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18
Q

Pseudomonas has a strictly - metabolism

A

Aerobic

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19
Q

Acinetobacter spp. Appear as ___ or __ on gram stain

A

gram-negative coccobacilli or gram-negative cocci

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20
Q

Acinetobacter organisms can resist decolonization and retain the ___ leading to misidentification.

A

crystal violet stain

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21
Q

Acinetobacter spp. are plump coc-cobacilli that tend to resist alcohol decolorization, thus
sometimes appearing

A

Gram positive

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22
Q

Colonies of acinetobacter appear as Smooth, opaque, raised, creamy, and smaller than Enterobacterales;
some genospecies are beta-hemolytic on what agar

A

Trypticase soy agar with 5% sheep blood

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23
Q

The genus acinetobacter is divided into two groups

A

saccharolytic, asaccharolytic

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24
Q

Glucose oxidizing

A

saccharolytic

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25
Q

non-glucose-utilizing

A

e asaccharolytic

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26
Q

Most glucose-oxidizing nonhemolytic strains were previously identified as

A

A. baumannii,

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27
Q

most non-glucose-utilizing, nonhemolytic strains were designated as

A

Acetobacter iwoffi

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28
Q

beta-hemolytic strains are identified as

A

Acinetobacter haemolyticus

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29
Q

is an oxidase-negative, nonfermentative, gram-negative bacillus

A

stenotrophomonas maltophilia

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30
Q

S. maltophilia oxidize ___ faster than glucose

A

Maltose

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31
Q

s. Maltophilia I can produce a __ pigment on ___agar that contains ___.

A

brown pigment pigment on brain-heart infusion agar that contains tyrosine.

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32
Q

medium that produces purplish hue in acinetobacter spp

A

MAC agar

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33
Q

an oxidase-negative, nonfermentative, gram-negative bacillus.

A

S. maltophilia

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34
Q

S. maltophilia Colonies may appear bluish on

A

MAC agar

35
Q

Which organism has large, smooth, glistening colonies
with a lavender pigment and smells like ammonia?

A

s. maltophilia

36
Q

Culture media for s. Maltophilia

A

BAP, MAC

37
Q

positive biochem test for s. maltophilia

A

Catalase, esculin, gelatin, hydrolysis, DNase, and LDC

38
Q

Antimicrobial Test: s maltophilia

A

Broth Microdilution and E-test

39
Q

B. Cepacia cause the media to undergo a color change from

A

red-orange to yellow.

40
Q

Burkholderia cepacia complex colonies on OFPBL agar change in color from ___because of the acidic pH caused by the utilization of lactose

A

green to yellow

41
Q

used to isolate B. pseudom-allei when melioidosis is suspect.

A

Ashdown medium

42
Q

b. Mallei morphology

A

Coccobacillus

43
Q

b. Pseudomallei morphology

A

small gram-negative rod with bipolar staining.

44
Q

Colonies of b. Cepacia are -and this trait may be used to differentiate isolates from P. stutzeri,

A

Non wrinkled

45
Q

B. cepacia produce a __ pigment

A

nonfluorescing yellow or green

46
Q

often produces a weak, slow, positive oxidase reaction

A

b cepacia

47
Q

B. cepacia strains oxidize

A

Glucose, maltose, lactose, mannitol

48
Q

BURKHOLDERIA CEPACIA Biochemical Tests:

A

weak positive oxidase reaction, (+) LDC and ONPG, most (+) ODC

49
Q

Burkholderia cepacia are mobile by means of

A

polar tufts of flagella

50
Q

Burkholderia mallei causes

A

Glanders

51
Q

nonmotile, gram-negative coccobacillus that produces nonpigmented colonies in 2 days.

A

B. mallei

52
Q

B. mallei morphology

A

is a nonmotile, gram-negative coccobacillus

53
Q

b mallei growth on __ and is variable

A

MAC agar and oxidase production

54
Q

b mallei oxidizes __

A

Glucose

55
Q

b mallei is ADH __ Arginine dihydrolase and Reduces nitrates to __

A

positive, reduces nitrates to nitrites

56
Q

Burkholderia pseudomallei causes

A

melioidosis/vietnamese timebomb

57
Q

Burkholderia pseudomallei demonstrates _ on Gram-stained smears.

A

bipolar staining

58
Q

Selective medium used for melioidosis that are deep pink

A

Ashdown medium with colistin

59
Q

The plant pathogen, Burkholderia gladioli resembles

A

B. cepacia complex

60
Q

Burkholderia gladioli produces __ pigment especially after 48 to 72 hours of incubation.

A

Yellow pigment

61
Q

B. gladioli grows on

A

MAC agar

62
Q

B. gladioli is difficult without the use of - for confirmation

A

molecular tools

63
Q

Alcaligenes faecalis and Achromobacter spp. possess ___ flagella

A

peritrichous flagella flagella

64
Q

Alcaligenes and Achromobacter usually grow well on most laboratory media, including

A

MAC agar.

65
Q

Alcaligenes faecalis in sheep blood agar appears as

A

Feather-edged colonies usually surrounded by zone of green discoloration

66
Q

Alcaligenes faecalis produce a highly characteristic odor

A

fruity odor resembling apples or strawberries

67
Q

grows in 6.5 NaCl broth

A

Alcaligenes faecalis

68
Q

Aerobic rod appears in coccoid shape.

A

Oligella spp

69
Q

genus Oligella comprises two asaccharolytic small coccobacilli species

A

O. ureolytica and O. urethralis.

70
Q

oligella spp motility

A

O. URETHRALIS (-)
O. UREOLTICA (+)

71
Q

Olligella oxidase test

A

O. URETHRALIS (+)
O. UREOLTICA (+)

72
Q

Oligella spp urease test

A

O. URETHRALIS (-)
O. UREOLTICA (+)

73
Q

procedure for detection of moraxella

A

Gram stain

74
Q

Moraxella lacunato appear as

A

coccobacilli or medium-sized rod.

75
Q

grow well 5% sheep blood and chocolate agars.

A

Moraxella spp.

76
Q

Moraxella lacunata appearance on sheep blood agar

A

Small gray to white colonies that pit the agar

77
Q

Moraxella lacunata on CHOC agar

A

Form dark halos

78
Q

moraxella lacunata on MAC

A

no growth

79
Q

Chromobacterium violaceum is motile for

A

polar flagella

80
Q

Chromobacterium violaceum produces a violet pigment about 91% of the time called

A

violacein

81
Q

Chromobacterium violaceum biochemical test

A

Variable oxidase

82
Q

Shewanella putrefaciens are Motile, assharolytic and a strong __ producer

A

H2S producer

83
Q

Shewanella putrefaciens biochem testv

A

(+) oxidase and ornithine decarboxylase

Clinical Bacteriology Lab (4 decks)

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