Non-pathogenic Intestinal Amoeba Flashcards
What are the non-pathogenic intestinal amoeba (8)
- Entamoeba coli
- Entamoeba dispar
- Entamoeba hartmanni
- Entamoeba gingivalis
- Entamoeba polecki
- Iodomoeba butschlii
- Endolimax nana
- Blastocystis hominis
The only pathogenic intestinal amoeba
Entamoeba histolytica
Entamoeba histolytica can be found in
Colon
Who discovered the Entamoeba histolytica in 1873 and named it Amoeba coli?
Dr. Fedor A. Lösch
Who discovered the Entamoeba histolytica in 1873 and named it Amoeba coli?
Dr. Fedor A. Lösch
Amoeba coli then was changed into Entamoeba histolytica by?
Fritz Schaudinn
The controversy between the species of Entamoeba histolytica was over:
● different size
● pathogenicity
● isoenzyme typing
● molecular techniques
It is alo known as the “small-raced E. histolytica”
Entamoeba hartmanni
He proposed that there are 2 types of Entamoeba histolytica (one pathogenic and one non-pathogenic)
Emile Brumpt
This technique allowed scientists to distinguish between pathogenic and non-pathogenic E. hisyolytica
Ppolymerase Chain Reaction - DNA and biogenetics
They named the E. histolytica into two separate species
Diamond and Clarke
What are the two type of Entamoeba that Clarke and Diamond named
E. histolytica - pathogenic
E. dispar - nonpathogenic
This is morphologically identical with Entamoeba hisyolytica but genetically different (DNA and ribosomal RNA)
Entamoeba dispar
This is morphologically identical with Entamoeba hisyolytica but genetically different (DNA and ribosomal RNA)
Entamoeba dispar
The only difference between Entamoeba dispar and Entamoeba histolytica is:
one base pair
Number of base pairs of E. histolytica
100
Number of base pairs of E. dispar
101
Cytoplasm of the E. dispar trophozoite
fine granular
What are the inclusions of the E. dispar trophozoites
- Bacteria
- NEVER contains RBC
- Bacteria or Debris in vacoules
The number of nucleus of E. dispar trophozoite
1 only
Describe the nucleus of the E. dispar trophozoite
● small and single with evenly distributed peripheral chromatin
Describe the karyosome of the E. dispar trophozoite
Small and Centrally located
What is the movememt of the E. dispar trophozoite
progressive and purposeful or directional
the only intestinal amoeba that is purposive (unidirectional)
E. dispar trophozoite
■ capable of endocytosis (ingestion orengulfment of substances or molecules) and exocytosis (excretion of substances; canalso be done via the plasma membrane)
E. dispar trophozoite
■ attachment to substrate
■ penetration through tissue (can lead toextraintestinal amoebiasis - liver - mostcommon, lungs, brain)
■ release of cytotoxic substances
■ contact cytolysis of pus cells - where theamoeba will attach itself to penetrate the tissues
E. dispar trophozoite
Inclusions for E. dispar cysts
Inclusions are usually observed in the YOUNG and IMMATURE CYSTS
how many nuclei does the mature have and immature have of E. dispar cysts
Mature - 4 nuclei
Immature - less than 4 nuclei
Ingested mature cyst of E.dispar can cause
Infection of E.histolytica or dispar
What can be tha cause/s of ingestion of cyst of E. dispar
● contaminated food
● contaminated water
● mechanical vectors (mosquieto and flies)
The infection of E. dispar/histolytica can cause two types of amebiasis namely:
- Intestinal Amebiasis
- Amebiasis characterized by dysentry
True or false:
E. dispar cyst are resistant to acid pH
TRUE
E.dispar cyst can survive for how many days at a certain temperature
● 2 days at 37° C
● 60 days at lower temperatures
E.dispar cysts are viable on:
● stool samples
● soil
● freshwater
● seawater
○ a week at 37°C
○ a month at 20°C
E.dispar chromatoidal bars:
present with rounded ends in young cysts
E.dispar cyst nucleus
1-4 nuclei
E.dispar cysts glycogen
present as DIFFUSE GLYCOGEN MASS in young cysts
When to report results as E.histolytica or E.dispar
● by microscopy
● by PCR
What should be reported if trophozoites contain ingested red blood cells?
● E.histolytica
● E.dispar
●both
E.histolytica
What should be reported if trophozoites contain ingested red blood cells?
● E.histolytica
● E.dispar
●both
E.histolytica
How should trophozoites be reported if they do not contain ingested red blood cells?
● E.histolytica
● E.dispar
●both
Both
