Neurohistology Flashcards
What different brain cell types are stained for by the Nissl stain?
All cells are detected. Only neuronal somata (cell bodies) are visible, but the nuclei of all cells visible
Which parts of neurons, glia, endothelial cells and rER of neurons are visible using the Nissl stain?
Nucleoli of neurons are visible, nucleus of neurons, glia and endothelial cells and the Nissl bodies of rER of neurons.
Which stain reveals the full morphology of neurons, showing exquisite detail of the dentritic arbour?
What colour does the stain look?
The golgi stain, which stains silver
Which stain is used to identify neurons given that they have very high density of rER (rough endoplsmic retiulum) in their somata?
Which other cell types does it stain for?
The Nissl stain is used to identify neurons due to the high density of rER in their somata. It actually stains all cell types but neurons are evident due to the concentrated rER.
Who is the myelin stain named after?
What colour does it stain?
The myelin, or Weigert stain, is used to identify oligodentrocyte processes, or myelin.
The cortical laminae are ordered 1-6. Which is the deepest layer, and what is it’s output?
Layer 6 is the deepest cortical layer, it outputs to the thalamus
What shape are golgi type 1 neurons, and how does this relate to their process?
Golgi type 1 neurons are pyramidal, which makes sense because they’re projection neurons
What shape are Golgi type 2 neurons, and what is their process?
Golgi type 2 neurons are stellate, and they are interneurons (eg LMNs)
Which layer of the cortical laminae receives input from the thalamus?
Layer 4 of the cortical laminae receives input from the thalamus
Which layer of the cortical laminae projects to the thalamus?
Layer 6 of the cortical laminae projects to the thalamus
Which layer of the cortical laminae projects to intrahemispheric cortex, and which layer projects to intrahemispheric AND interhemispheric cortex?
Layer 2 projects to intrahemispheric cortex whilst layer 3 projects to intrahemispheric and interhemispheric cortex
Which cortical laminae layer projects to the contralateral cortex?
Layer 3 projects to the contralateral cortex
Which cortical laminae layer projects to subcortical centres and the spinal cord?
Layer 5 projects to subcortical centres and the spinal cord
