Nage 4

Question 1

What is preRNA / hnRNA

Answer 1

Transcribed RNA before it has been modified

Question 2

Where does RNA processing occur

Answer 2

In the nucleus

Question 3

What is the splice donor site and how do you recognise it

Answer 3

AAGU - where the snRNP U1 binds and it cleaves the bond between the A and the G

Question 4

What is the complex of the snRNP binding onto the intron called

Answer 4

Splicesosome

Question 5

What does snRNP stand for

Answer 5

Small Ribonuclear protein

Question 6

Which snRNP bind onto the splice acceptor site and how do you recognise this site

Answer 6

U5 , pyr15NCAG

Question 7

Explain happens to the intron when the spliceosome has formed

Answer 7

The Splice acceptor site is cleaved, the GU From the beginning of the intron binds onto the A residue, and the splice donor site is then cleaved, forming a lariat

Question 8

Where do the snRNP’s bind onto?

Answer 8

U1 binds onto the splice acceptor site
U2, U4 and U6 binds onto the intron
U5 binds onto the splice donor site

Question 9

Explain the addition of the CAP

Answer 9

A guanosine triphosphate nucleoside is added onto the 5’ end of an mRNA chain with a special 5’-5’ bond, which prevents the mRNA from degradation.