NA 3 Flashcards

1
Q

Where/when does dna synthesis occur in prokaryotes

A

In the cytoplasm during cell division

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2
Q

How does helicase denature DNA

A

Via hydrolysis

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3
Q

What end do Deoxyriboucleotides get added on the newly synthesized strand

A

3’

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4
Q

How many dna polymerases does E. Coli have

A

5

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5
Q

How many dna polymerases do humans have

A

More than 15

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6
Q

Which polymerases do the majority of eukaryotic nuclear
DNA synthesis

A

δ and ε

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7
Q

What is the role of Topoisomerase

A

cleaves DNA to adjust negative supercoils

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8
Q

What is the difference between type 1 and type 2 Topoisomerase

A

Type 1 cuts one strand and type 2 cuts both

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9
Q

What is the role of Single-strand binding (SSB) proteins

A

stabilize DNA and prevent annealing

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10
Q

What is the role primase

A

enzyme that synthesizes short segments of RNA (i.e. primers)

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11
Q

What is the role of ligase

A

enzyme that joins two DNA fragments together via ATP hydrolysis

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12
Q

T/f, prokaryotic dna polymerases have primase activity

A

False; eukaryotic DNA polymerase α does

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13
Q

Eukaryotic dna replication is terminated by

A

Collision of replication forks

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14
Q

What is telomerase

A

Polymerase that synthesizes and maintain telomeres

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15
Q

An adenine for a guanine is an example of what mutation

A

Transition

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16
Q

A purine substituted for a pyrimidine is what kind of mutation

A

Transverse

17
Q

A silent mutation is when an mRNA codon calls for ______ amino acid

A

Same

18
Q

Missense mutation calls for ______ amino acid

A

Different

19
Q

T/f, exons code for a protein

A

True