NA 3 Flashcards
Where/when does dna synthesis occur in prokaryotes
In the cytoplasm during cell division
How does helicase denature DNA
Via hydrolysis
What end do Deoxyriboucleotides get added on the newly synthesized strand
3’
How many dna polymerases does E. Coli have
5
How many dna polymerases do humans have
More than 15
Which polymerases do the majority of eukaryotic nuclear
DNA synthesis
δ and ε
What is the role of Topoisomerase
cleaves DNA to adjust negative supercoils
What is the difference between type 1 and type 2 Topoisomerase
Type 1 cuts one strand and type 2 cuts both
What is the role of Single-strand binding (SSB) proteins
stabilize DNA and prevent annealing
What is the role primase
enzyme that synthesizes short segments of RNA (i.e. primers)
What is the role of ligase
enzyme that joins two DNA fragments together via ATP hydrolysis
T/f, prokaryotic dna polymerases have primase activity
False; eukaryotic DNA polymerase α does
Eukaryotic dna replication is terminated by
Collision of replication forks
What is telomerase
Polymerase that synthesizes and maintain telomeres
An adenine for a guanine is an example of what mutation
Transition