MYCOLOGY LAB ID METHODS Flashcards
what is the gold standard of mycology id methods
culture
what newer diagnostic testing is mostly used for yeast
MALDI-TOF
what newer diagnostic testing is mostly used for bacteria
micro scan, vitex, and phoenix
what kind of lab uses gram stain, modified acid fast stain, wright stain, and giemsa stain
clinical lab
what kind of lab uses periodic acid-schiff, H & E, Aomori’s methenamine silver
pathology lab
T. or F: calcofluor white is used for fluorescence microscopy
T
what makes up the KOH wet mount
10% potassium hydroxide and specimen
what is the purpose of the KOH wet mount
dissolve away the non fungal elements
is a KOH met mount stained
no
T or F: KOH wet mount can be examined hours after it is made
F
what is the purpose of India ink
to ID encapsulated yeast
what yeast does India ink ID
cryptococcus
what kind of specimen does India ink use
CSF
what stain is used to ID fungal growth on agar
lactophenol cotton blue
lactophenol cotton blue: what is the purpose of phenol
kills organisms
lactophenol cotton blue: what is the purpose of lactic acid
preserves fungal structures
lactophenol cotton blue: what is the purpose of cotton blue
stains the chitin in the cell wall
what are the 2 methods used to pick up fungus off agar
tape or tease mount
what does calcofluor white bind to
polysaccharides of chitin in fungal cell wall
T or F: calcofluor white works on any fungus
F
what kind of morphology can be examined from culture
micro and macroscopic
what is the primary inoculation of culture
specimen on the agar plate
what is the secondary. isolation of culture
put colonies onto a new type of media
what kind of organism does chromogenic agar ID
yeast
what kind of organism does cornmeal agar ID
C. albicans chlamydospores
what kind of organism does bird seed agar ID
cryptococcus
what does some media have to prevent overgrowth of bacteria from mixed cultures
antibacterial ingredients
what do microscans ID
yeast
what does microscan use to ID organisms
substrate, carbohydrate, and enzyme differentiation
what is the principle of DNA probes
nucleic acid hybridization
how do you know if there are ab present in immunodiffusion testing
a line of precipitation appears
is immunodiffusion qualitative or quantitative
qualitative
in complement fixation testing is the is no color change what does that mean
there are fungal Ab in pt serum –> complement does not lyse sheep blood –> pos
in complement fixation testing is the is a color change what does that mean
there are not fungal Ab in pt serum –> complement does lyse sheep blood –> neg
what is the purpose of antifungal susceptibility testing
produce MIC for pt therapy, inform epidemiological studies, and track drug resistance
when will a doc request antifungal susceptibility testing
invasive fungal infection, if drug resistance is suspected, or pt theory is not working
if there is no growth around an antibiotic the organism is ______
sensitive to that antibiotic
if there is growth around an antibiotic the organism is ______
resistant to that antibiotic
if a fungus does have some zone of no growth but there is a little what is that called
trailing growth
what organism does triazole treat
aspergillus
