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VIROLOGY > MYCOLOGY LAB ID METHODS > Flashcards

MYCOLOGY LAB ID METHODS Flashcards

1
Q

what is the gold standard of mycology id methods

A

culture

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2
Q

what newer diagnostic testing is mostly used for yeast

A

MALDI-TOF

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3
Q

what newer diagnostic testing is mostly used for bacteria

A

micro scan, vitex, and phoenix

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4
Q

what kind of lab uses gram stain, modified acid fast stain, wright stain, and giemsa stain

A

clinical lab

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5
Q

what kind of lab uses periodic acid-schiff, H & E, Aomori’s methenamine silver

A

pathology lab

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6
Q

T. or F: calcofluor white is used for fluorescence microscopy

A

T

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7
Q

what makes up the KOH wet mount

A

10% potassium hydroxide and specimen

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8
Q

what is the purpose of the KOH wet mount

A

dissolve away the non fungal elements

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9
Q

is a KOH met mount stained

A

no

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10
Q

T or F: KOH wet mount can be examined hours after it is made

A

F

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11
Q

what is the purpose of India ink

A

to ID encapsulated yeast

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12
Q

what yeast does India ink ID

A

cryptococcus

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13
Q

what kind of specimen does India ink use

A

CSF

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14
Q

what stain is used to ID fungal growth on agar

A

lactophenol cotton blue

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15
Q

lactophenol cotton blue: what is the purpose of phenol

A

kills organisms

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16
Q

lactophenol cotton blue: what is the purpose of lactic acid

A

preserves fungal structures

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17
Q

lactophenol cotton blue: what is the purpose of cotton blue

A

stains the chitin in the cell wall

18
Q

what are the 2 methods used to pick up fungus off agar

A

tape or tease mount

19
Q

what does calcofluor white bind to

A

polysaccharides of chitin in fungal cell wall

20
Q

T or F: calcofluor white works on any fungus

A

F

21
Q

what kind of morphology can be examined from culture

A

micro and macroscopic

22
Q

what is the primary inoculation of culture

A

specimen on the agar plate

23
Q

what is the secondary. isolation of culture

A

put colonies onto a new type of media

24
Q

what kind of organism does chromogenic agar ID

A

yeast

25
Q

what kind of organism does cornmeal agar ID

A

C. albicans chlamydospores

26
Q

what kind of organism does bird seed agar ID

A

cryptococcus

27
Q

what does some media have to prevent overgrowth of bacteria from mixed cultures

A

antibacterial ingredients

28
Q

what do microscans ID

A

yeast

29
Q

what does microscan use to ID organisms

A

substrate, carbohydrate, and enzyme differentiation

30
Q

what is the principle of DNA probes

A

nucleic acid hybridization

31
Q

how do you know if there are ab present in immunodiffusion testing

A

a line of precipitation appears

32
Q

is immunodiffusion qualitative or quantitative

A

qualitative

33
Q

in complement fixation testing is the is no color change what does that mean

A

there are fungal Ab in pt serum –> complement does not lyse sheep blood –> pos

34
Q

in complement fixation testing is the is a color change what does that mean

A

there are not fungal Ab in pt serum –> complement does lyse sheep blood –> neg

35
Q

what is the purpose of antifungal susceptibility testing

A

produce MIC for pt therapy, inform epidemiological studies, and track drug resistance

36
Q

when will a doc request antifungal susceptibility testing

A

invasive fungal infection, if drug resistance is suspected, or pt theory is not working

37
Q

if there is no growth around an antibiotic the organism is ______

A

sensitive to that antibiotic

38
Q

if there is growth around an antibiotic the organism is ______

A

resistant to that antibiotic

39
Q

if a fungus does have some zone of no growth but there is a little what is that called

A

trailing growth

40
Q

what organism does triazole treat

A

aspergillus

VIROLOGY (6 decks)

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