Mycobacterium Flashcards
Negative for T 80 HOH
M. avium
Slow growers except?
M. fortuitum
M. chelonei
AFB (mycolic acid)
true
Middlebrook 7H10-7H11-AST
Agar base
LJ Medium
Egg base
1+
10-99 AFB per 100 fields
Inhibitor of Egg base medium
Malachite Green
M. avium complex
Lady Windermere syndrome
Intestinal TB
MB bovis
Duboi’s oleic acid albumin medium
Agar base
Negative for HSCT
M. TB
BACTEC 12 B
Liquid base
Source of intestinal TB
cattle
3+
> 10 per field in at least 20 fields
Acid Fast stain
AFB grading national Std.
Dorset
Egg base
Result of HSCT
> 45mm height of gas bubbles
Middle brook 7H9
Liquid base
Positive for T 80 HOH
M. kansasii
Principle of Niacin Test
Niacin + Niacin Ribonucleotide + aniline dye + cyanogens bromide
Reduction positive
M. avium
+n
1-9 AFB per 100 fields
American thoracic society medium
Egg base
6% Oxalic Acid is for?
Specimen contaminated with Pseudomonas like urine and stool
Other name of MB TB
Koch bacillus
specimen for nitrate reduction test
avium +
kansasii +
Anaerobe or aerobe?
Obligate AEROBE
Positive for TRT
M. avium
Best medium for NIACIN test
LJ medium
Anaerobic
Non spore former
Resist Acid Alcohol
true
Mitchison Medium
Agar base
2+
1-10 AFB per fields in at least 50 fields
Petragnani Medium
Egg base
3 Nitrate Reduction Test chemicals
HCl
Sulfanilamide
n-napthulethylene diamine
tween 80 hoh
kansasii
avium
For heavily contaminated specimens
Petragnani medium
Egg base Medium
Highly Proteinized
Don’t Autoclave
Positive for HSCT
M. kansasii
hsct
kansasii
tb
Carbon source for LJ medium
Glycerin
arylsulfatase
fortuitum +
chelonei +
Gram Stain is for?
Decontamination/Digestion
Positive for NRT
M. avium
non specific to MTB
LJ medium
Ideal size of smear
2cm by 3
It produces what?
Much granules
Positive Niacin test
MTB
Negative Niacin Test
M. bovis
Meaning of NALC
N-Acetyl L-Cysteine
Semi quantitative
heat stable catalase test
What other spp. can grow on LJ medium?
Nocardia
Reagent for HSCT
30% H2O2
Medium for HSCT
Tween 80
No. 1 NTM in AIDS patients
M. avium
Niacin test
tb
bovis
Result of Tellurite Reduction Test
Black Metallic
Principle of Tween 80 HOH test
Tween 80 —> HOH of tween 80
Result for NRT
Pink or Yellow
Result of Tween 80 HOH test
Red
Heat Stable Catalase Test
Tween 80 + MB + 30% H2O2 + 68 deg
Tween 80 positive
M. kansasii
Negative for TRT
M. kansasii
tellurite reduction test
avium kansasii
Principle of Tellurite reduction test
tellurite –> black metallic tellurium
% of NALC-NaOH
2 to 3%
3 groups of Mycobacterium
MB Tuberculosis Complex
MOTT
MB leprae
check in _____ fields before reporting
300
best for digestion
NALC NaOH + Na Citrate
For digestion
NALC NaOH + Na Citrate
Zephiran Trisodium PO4
6% Oxalic Acid
Dithiothreitol
Bottle number for CSF
2
Result of Niacin test
yellow
how to sterilize LJ medium?
Inspissation
70 to 80 deg for 2 hours
temp for HSCT
68 deg
Nitrate to Nitrite
Nitrate reduction test
Cannot be grown on agar
MB leprae
Automated System
Liquid Media
Best with glycerin
LJ medium
Septi Check
Liquid base
Vaccine for TB
Bacillus Calmette Gwerin
Pulmonary TB
Tuberculosis
Africanum
Virulence for MB TB
Cord factor
Sulfatides
How to sterilize loop after using?
Dip in lysol then in sand
0
no AFB per 300 fields
