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Bacte Midterms > Mycobacterium > Flashcards

Mycobacterium Flashcards

0
Q

Negative for T 80 HOH

A

M. avium

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1
Q

Slow growers except?

A

M. fortuitum

M. chelonei

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2
Q

AFB (mycolic acid)

A

true

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3
Q

Middlebrook 7H10-7H11-AST

A

Agar base

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5
Q

LJ Medium

A

Egg base

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6
Q

1+

A

10-99 AFB per 100 fields

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7
Q

Inhibitor of Egg base medium

A

Malachite Green

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10
Q

M. avium complex

A

Lady Windermere syndrome

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11
Q

Intestinal TB

A

MB bovis

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12
Q

Duboi’s oleic acid albumin medium

A

Agar base

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14
Q

Negative for HSCT

A

M. TB

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16
Q

BACTEC 12 B

A

Liquid base

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17
Q

Source of intestinal TB

A

cattle

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19
Q

3+

A

> 10 per field in at least 20 fields

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20
Q

Acid Fast stain

A

AFB grading national Std.

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22
Q

Dorset

A

Egg base

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23
Q

Result of HSCT

A

> 45mm height of gas bubbles

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25
Q

Middle brook 7H9

A

Liquid base

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26
Q

Positive for T 80 HOH

A

M. kansasii

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28
Q

Principle of Niacin Test

A

Niacin + Niacin Ribonucleotide + aniline dye + cyanogens bromide

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29
Q

Reduction positive

A

M. avium

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30
Q

+n

A

1-9 AFB per 100 fields

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32
Q

American thoracic society medium

A

Egg base

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34
Q

6% Oxalic Acid is for?

A

Specimen contaminated with Pseudomonas like urine and stool

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37
Q

Other name of MB TB

A

Koch bacillus

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38
Q

specimen for nitrate reduction test

A

avium +

kansasii +

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40
Q

Anaerobe or aerobe?

A

Obligate AEROBE

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42
Q

Positive for TRT

A

M. avium

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43
Q

Best medium for NIACIN test

A

LJ medium

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45
Q

Anaerobic
Non spore former
Resist Acid Alcohol

A

true

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47
Q

Mitchison Medium

A

Agar base

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48
Q

2+

A

1-10 AFB per fields in at least 50 fields

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50
Q

Petragnani Medium

A

Egg base

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52
Q

3 Nitrate Reduction Test chemicals

A

HCl
Sulfanilamide
n-napthulethylene diamine

53
Q

tween 80 hoh

A

kansasii

avium

54
Q

For heavily contaminated specimens

A

Petragnani medium

55
Q

Egg base Medium

A

Highly Proteinized

Don’t Autoclave

56
Q

Positive for HSCT

A

M. kansasii

57
Q

hsct

A

kansasii

tb

58
Q

Carbon source for LJ medium

A

Glycerin

59
Q

arylsulfatase

A

fortuitum +

chelonei +

60
Q

Gram Stain is for?

A

Decontamination/Digestion

61
Q

Positive for NRT

A

M. avium

62
Q

non specific to MTB

A

LJ medium

63
Q

Ideal size of smear

A

2cm by 3

64
Q

It produces what?

A

Much granules

65
Q

Positive Niacin test

A

MTB

66
Q

Negative Niacin Test

A

M. bovis

67
Q

Meaning of NALC

A

N-Acetyl L-Cysteine

69
Q

Semi quantitative

A

heat stable catalase test

70
Q

What other spp. can grow on LJ medium?

A

Nocardia

71
Q

Reagent for HSCT

A

30% H2O2

72
Q

Medium for HSCT

A

Tween 80

73
Q

No. 1 NTM in AIDS patients

A

M. avium

74
Q

Niacin test

A

tb

bovis

75
Q

Result of Tellurite Reduction Test

A

Black Metallic

76
Q

Principle of Tween 80 HOH test

A

Tween 80 —> HOH of tween 80

77
Q

Result for NRT

A

Pink or Yellow

78
Q

Result of Tween 80 HOH test

A

Red

79
Q

Heat Stable Catalase Test

A

Tween 80 + MB + 30% H2O2 + 68 deg

80
Q

Tween 80 positive

A

M. kansasii

82
Q

Negative for TRT

A

M. kansasii

83
Q

tellurite reduction test

A

avium kansasii

84
Q

Principle of Tellurite reduction test

A

tellurite –> black metallic tellurium

85
Q

% of NALC-NaOH

A

2 to 3%

86
Q

3 groups of Mycobacterium

A

MB Tuberculosis Complex
MOTT
MB leprae

87
Q

check in _____ fields before reporting

A

300

88
Q

best for digestion

A

NALC NaOH + Na Citrate

89
Q

For digestion

A

NALC NaOH + Na Citrate
Zephiran Trisodium PO4
6% Oxalic Acid
Dithiothreitol

92
Q

Bottle number for CSF

A

2

93
Q

Result of Niacin test

A

yellow

97
Q

how to sterilize LJ medium?

A

Inspissation

70 to 80 deg for 2 hours

101
Q

temp for HSCT

A

68 deg

102
Q

Nitrate to Nitrite

A

Nitrate reduction test

111
Q

Cannot be grown on agar

A

MB leprae

112
Q

Automated System

A

Liquid Media

114
Q

Best with glycerin

A

LJ medium

115
Q

Septi Check

A

Liquid base

117
Q

Vaccine for TB

A

Bacillus Calmette Gwerin

124
Q

Pulmonary TB

A

Tuberculosis

Africanum

135
Q

Virulence for MB TB

A

Cord factor

Sulfatides

136
Q

How to sterilize loop after using?

A

Dip in lysol then in sand

138
Q

0

A

no AFB per 300 fields

Bacte Midterms (9 decks)

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