Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacte > Mycobacteria > Flashcards

Mycobacteria Flashcards

1
Q
  • aerobic bacilli
  • difficult to decolorize (acid-fast)
  • grow slowly
A

Mycobacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Identification of Mycobacteria

A
  1. colony morphology
  2. colony pigmentation
  3. growth rate
  4. growth temperture
  5. biochemical test
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

decontamination and digestion agents

A

sodium hydroxide
N-acetyl-L-cyteine
benzalkonium chloride
oxalic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

mycobacterial growth media

A
  1. Lowenstein-Jensen - egg-based medium

2. Middlebrook 7H10 - agar based; inhibitory to contaminants

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Mycobacterial stain

A
  1. conventional stains - Ziehl-Neelsen and Kinyoun

2. fluorochrome stains - auramine and auramine-rhodamine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

1 stain: carbolfuchsin
decolorizing agent: acid alcohol
counterstain: methylene blue

A

Ziehl-Neelsen and Kinyoun

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

produce pigment on exposure to light

A

photochromogens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

produce pigment in the light or dark

A

Scotochromogens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

no pigment produced in the light or dark

A

nonchromogens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

produce colonies in <= 7 days

A

rapid growers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

produce colonies in >= 7 days

A

slow growers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

growth temperature

A

35-37C

  • M. marinum - 30C
  • M. ulcerans - 32C
  • M. xenopi - 42C
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Biochemical tests

A
  1. Niacin accumulation
  2. Nitrate reduction
  3. Catalase
  4. Tween hydrolysis
  5. NaCl tolerance
  6. Iron uptake
  7. Arylsulfatase
  8. Urease test
  9. Susceptibility to thiophen-2-carboxylic acid hydrazide
    10 nucleic acid amplification
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

measures nicotinic acid w/c reacts with cyanogen bromide in the presence aniline to form a yellow compound

A

Niacin accumulation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q
  • used for Enterobacteriaceae

- end product: red pigment

A

Nitrate reduction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

measures the height of the column of bubbles when H2O2 is added

A

Catalase

17
Q
  • measures the presence of lipase

- pink color change

A

Tween hydrolysis

18
Q

uses egg-based media w/ 5% NaCl

A

NaCl tolerance

19
Q

determined by adding ferric ammonium citrate to the colonies and observing rusty-brown colonies (formation of iron oxide)

A

Iron uptake

20
Q
  • adds phenolphthalein to the colony/substrate mixture

- pink color

A

arylsulfatase

21
Q
  • performed and read as broth test
  • urea to ammonia
  • alkaline (increase in pH): red color
A

Urease test

22
Q

determined by observing growth or no growth

A

Susceptibility to thiophen-2-carboxylic acid hydrazide

23
Q

cutaneous infections from water exposure

A

M. marinum

24
Q

common contaminant (tap-water bacillus)

A

M. gordonae

25
Q

cervical lymphadenitis in children

A

M. scrofulaceum

26
Q

contaminant

A

M. flavescens

27
Q

Photochromogen:

  1. M. marinum
  2. M. kansasii
  3. M. simiae
A

M. kansasii:
(-) Niacin
(+) Nitrate reduction

28
Q

Nonchromogen:

  1. M. tuberculosis
  2. M. bovis
  3. M. avium - M. intracellulare
A

M. tuberculosis: (+) Niacin, Nitrate reduction
M. bovis: (+) susceptible to TCH
M. avium - M. intracellulare: all (-) biochemical tests

29
Q

Rapid grower:

  1. M. fortuitum
  2. M. chelonei
A

M. fortuitum: (+) Nitrate reduction, Iron uptake

M. chelonei: (-) Nitrate reduction, Iron uptake

30
Q

Scotochromogen:

  1. M. gordonae
  2. M. scrofulaceum
  3. M. szulgai
  4. M. xenopi
  5. M. flavescens
A

M. flavescens: (+) growth in 5% NaCl

31
Q

(-) all biochemical tests

A

M. avium - M. intracellulare

32
Q

(+) susceptible to TCH

A

M. bovis

33
Q

growth on MacConkey agar

A

Rapid grower:

  1. M. fortuitum
  2. M. chelonei
34
Q

growth on Tellurite

A

M. avium

* variable: rapid grower (M. fortuitum, M. chelonei)

35
Q

(+) Iron uptake

A

M. fortuitum

36
Q

growth in 5% NaCl

A

M. flavescens
M. fortuitum
* variable: M. chelonei

37
Q

(+) Niacin

A

M. marinum
M. simiae
M. tuberculosis
* variable: M. chelonei

Bacte (2 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions