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Bacte > Media > Flashcards

Media Flashcards

1
Q

Media in a gel or semi-solid form

A

Agar

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2
Q

Nutrient agar, trypticase soy agar, nutrient broth

A

General isolation media/ supportive media

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3
Q

Sheep blood agar (sba), chocolate agar

A

Nonselective isolation media

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4
Q

Trypticase soy agar, Brucella agar, or beef heart infusion

with 5% sheep blood

A

Sheep blood agar

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5
Q

Agar is heated or enzyme treated to hemolyzed rbc –> release NAD V factor) and hemin ( X factor)

A

Chocolate agar

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6
Q

MacConkey agar, Eosin-Methylene blue (emb)

A

Differential media

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7
Q

Peptone base with lactose; gram-positive organisms
inhibited by crystal violet and bile salts; neutral red as
indicator

A

MacConkey agar

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8
Q

Peptone base containing lactose; eosin Y and methylene

blue as indicators

A

Eosin-methylene blue (emb)

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9
Q

Gram negative broth, selenite broth, tetrathionate broth, thioglycollate broth

A

Enrichment broth

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10
Q

Peptone base broth with glucose and mannitol (primary CHO); sodium
citrate and sodium desoxycholate (bile salt) act as inhibitory
agents

A

Gram negative broth

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11
Q

Contains sodium hydrogen selenite (toxic for most
Enterobacteriaceae)
Enhance isolation of Salmonella and Shigella

A

Selelnite broth

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12
Q

Peptone base broth; iodine and potassium iodide, bile
salts, and sodium thiosulfate inhibit gram-positive
organisms and Enterobacteriaceae

A

Tetrathionate broth

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13
Q

Pancreatic digest of casein, soy broth, and glucose enrich
growth of most microorganisms; includes reducing
agents thioglycolate, cystine, and sodium sulfite;
semisolid medium with a low concentration of agar
reducing oxygen diffusion in the medium; yeast and beef extracts, vit K1, hemin, resazurin (redox indicator)

A

Thioglycollate broth

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14
Q

Hektoen enteric (he) agar, Salmonella-Shigella (ss) agar, xylose-lysine-deoxycholate (xld) agar

A

Selective media

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15
Q

Contains lactose, sucrose, salicin, sodium thiosulfate, ferric ammonium citrate, bromthymol blue and acid fuchsin as indicator
Fermenter cho = yellow-orange
Nonfermenter = green or blue
Hydrogen sulfide producing = black ppt (Salmonella)

A

Hektoen enteric (he) agar

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16
Q

Agar

Supports growth of N. gonorrhea and H. Influenzae

A

Chcolate agar

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17
Q

Media in liquid form

A

Broth

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18
Q

Peptone base with lactose, ferric citrate, and sodium
citrate; neutral red as indicator; inhibition of coliforms
by brilliant green and bile salts

A

Salmonella-Shigella (ss) agar

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19
Q

Colistin-nalidixic acid (cna), modified thayer-martin (mtm)

A

Antibiotic media

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20
Q 
Chocolate base agar, supplement B, vancomycin, colistin, nystatin, trimethoprim lactate
Vancomycin = inhibits gram positive
Colistin = inhibits gram negative
Nystatin = inhibits yeast
Trimethoprim = inhibits Proteus
A

Modified thayer martin (mtm)

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21
Q

Similar to modified thayer martin (mtm) but anisomycin as yeast inhibitor

A

Martin lewis medium

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22
Q

Support the growth of most nonfastidious bacteria

A

General isolation media/ supportive media

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23
Q

Contain a nutrient supplement

A

Nonselective isolation media

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24
Q

provide distinct colonial appearances of microorganisms to aid in their identification

A

Differential agar

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25
Q 
Peptone base, enriched with solution of 2% hemoglobin
or IsoVitaleX (BBL) -dextrose, cysteine, vit B12, thiamine, ferric nitrate
A

Chocolate agar

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26
Q

Supports growth of anaerobes, aerobes (Pseudomonas),

microaerophilic, and fastidious microorganisms

A

Thioglycollate broth

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27
Q

Isolation and differentiation of lactose fermenting (pink to red)
and non–lactose-fermenting (clear) enteric bacilli (Shigella)

A

MacConkey Agar

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28
Q

In certain bacteria, exceptional media components must be used to grow

A

Fastidious bacteria

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29
Q

Turbidity in broth

A

A least 10^6 bacteria per mL

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30
Q

Type of media

used to isolate gram negative

A

Differential media

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31
Q

Yeast extract agar with lysine, xylose, lactose, sucrose,
and ferric ammonium citrate; sodium desoxycholate
inhibits gram-positive organisms; phenol red as
indicator
Xylose fermentation
Lysine dacarboxylation
Hydrogen sulfide production

A

Xylose-lysine-deoxycholate (xld) agar

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32
Q

Type of media

isolation of stool pathogens

A

Selective media

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33
Q

selective inhibit gram positive bacteria and gram negative coliform bacteria

A

Selective media

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34
Q

Selective for N. gonorrhoeae and N. meningitidis.
Supports the growth of Francisella and Brucella
spp.

A

Modified thayer martin (mtm)

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35
Q

Used to inhibit the growth of one organism while enhancing that of another organi

A

Enrichment broth

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36
Q

Process growing microorganisms in culture

A

Cultivation

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37
Q

Used most frequently for stool cultures to inhibit normal flora

A

Enrichment broth

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38
Q

Isolation and differentiation of lactose-fermenting (purple)

and non–lactose-fermenting (clear) enteric bacilli

A

Eosin-Methylene blue (emb)

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39
Q

Contains sheep blood agar base, colistin (polymyxin e), nalidixic acid
Colistin = disrupt cell membrane of gram negative bacteria
Nalidixic acid = blocks dna replication in gram negative bacteria

A

Colistin-nalidixic acid (cna)

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40
Q

Yeast extract, agar, charcoal, and salts supplemented with
L-cysteine HCl, ferric pyrophosphate, ACES buffer, and
α-ketoglutarate

A

buffered charcoal-yeast extract agar (BCYE)

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41
Q

for selective enrichment of

group B streptococci

A

LIM broth

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42
Q

nutrient medium for

fungi

A

Sabouraud’s dextrose agar

43
Q

selective medium that inhibits the
growth of aerobic and facultatively anaerobic gramnegative
rods and allows gram-positive cocci to grow

A

phenylethyl alcohol (PEA) agar

44
Q

Nutrient agar base with ferric citrate. Hydrolysis of esculin
by group D streptococci imparts a brown color to
medium; sodium desoxycholate inhibits many bacteria

A

Bile esculin agar (BEA)

45
Q

Differential isolation and presumptive identification

of group D streptococci and enterococci

A

Bile esculin agar (BEA)

46
Q

Contains azide to inhibit gram-negative bacteria,
vancomycin to select for resistant gram-positive
bacteria, and bile esculin to differentiate enterococci
from other vancomycin-resistant bacteria that may
grow

A

Bile esculin azide agar with

vancomycin

47
Q

Selective and differential for cultivation of
vancomycin-resistant enterococci from clinical
and surveillance specimens

A

Bile esculin azide agar with

vancomycin

48
Q

Potato-glycerol–based medium enriched with 15%-20%
defibrinated blood; contaminants inhibited by
methicillin (final concentration of
2.5 μm/mL)

A

Bordet-Gengou agar

49
Q

Isolation of Bordetella pertussis and Bordetella

parapertussis

A

Bordet-Gengou agar

50
Q

Dextrose, pork brain and heart dehydrated infusions.

A

Brain heart infusion agar or

broth

51
Q

Cultivation of fastidious organisms.

A

Brain heart infusion agar or

broth

52
Q

Enrichment for Legionella spp.
Supports the growth of Francisella and Nocardia
spp.

A 
Buffered charcoal-yeast
extract agar (BCYE)
53
Q

BCYE supplemented with polymyxin B, vancomycin, and
ansamycin, to inhibit gram-negative bacteria,
gram-positive bacteria, and yeast, respectively

A

Buffered charcoal-yeast
extract (BCYE) agar with
antibiotics

54
Q

Enrichment and selection for Legionella spp.

A

Buffered charcoal-yeast
extract (BCYE) agar with
antibiotics

55
Q

Bile salts, gentamycin, ticarcillin, polymixin B, Peptone,

yeast extract

A

Burkholderia cepacia selective

agar

56
Q

For recovery of B. Cepacia from cystic fibrosis

patients

A

Burkholderia cepacia selective

agar

57
Q

Contains vancomycin (10 mg/L), trimethoprim
(5 mg/L), polymyxin B (2500 U/L), amphotericin B
(2 mg/L), and cephalothin (15 mg/L) in a Brucella agar
base with sheep blood

A

Campy-blood agar

58
Q

Selective for Campylobacter spp.

A

Campy-blood agar

59
Q

Thioglycollate broth supplemented with increased agar

concentration and antibiotics

A

Campylobacter thioglycollate

broth

60
Q

Selective holding medium for recovery of
Campylobacter spp.
Incubated at 4° C for cold-enrichment.

A

Campylobacter thioglycollate

broth

61
Q

Tryptic soy broth, 5% sheep blood and added nutrients

A

CDC anaerobe 5% sheep

blood agar

62
Q

Improved growth of obligate, slow-growing

anaerobes

A

CDC anaerobe 5% sheep

blood agar

63
Q

Blood-supplemented enrichment medium containing
cefoperazone, vancomycin, and amphotericin to inhibit
growth of most gram-negative bacteria, gram-positive
bacteria, and yeast, respectively

A

Cefoperazone, vancomycin,
amphotericin (CVA)
medium

64
Q

Selective medium for isolation of Campylobacter

spp.

A

Cefoperazone, vancomycin,
amphotericin (CVA)
medium

65
Q

Peptone base with yeast extract, mannitol, and bile salts;
supplemented with cefsulodin, irgasan, and novobiocin;
neutral red and crystal violet indicators

A

Cefsulodin-irgasannovobiocin

(CIN) agar

66
Q

Selective for Yersinia spp.; may be useful for

isolation of Aeromonas spp.

A

Cefsulodin-irgasannovobiocin

(CIN) agar

67
Q

Cultivation of fastidious microorganisms such as
Haemophilus spp., Brucella spp. and pathogenic
Neisseria spp.

A

Chocolate agar

68
Q

Organism-specific nutrient base, selective supplements

and chromogenic substrate

A

Chromogenic media

69
Q

Chromogenic media are designed to optimize
growth and differentiate a specific type
of organism. Chromagars are routinely used in
the identification of yeasts, methicillin-resistant
Stapylococcus aureus (MRSA), and a variety of
other organisms.

A

Chromogenic media

70
Q

Columbia agar base with 10 mg colistin per liter, 15 mg

nalidixic acid per liter, and 5% defibrinated (removed fibrin) sheep blood

A

Columbia colistin-nalidixic

acid (CNA) agar

71
Q

Selective isolation of gram-positive cocci

A

Columbia colistin-nalidixic

acid (CNA) agar

72
Q

Infusion agar base with 5% sheep blood; reduction of
potassium tellurite by Corynebacterium diphtheriae
produces black colonies

A

Cystine-tellurite blood agar

73
Q

Isolation of C. diphtheriae

A

Cystine-tellurite blood agar

74
Q

Selective (enrichment) liquid medium for enteric

pathogens

A

Gram-negative broth (GN)

75
Q

Differential, selective medium for the isolation and
differentiation of Salmonella and Shigella spp.
from other gram-negative enteric bacilli

A

Hektoen enteric (HE) agar

76
Q 
Animal tissue (heart muscle), dextrose, eggs and beef
serum, and sodium chloride
A

Loeffler’s medium

77
Q

Isolation and growth of Corynebacterium

A

Loeffler’s medium

78
Q

A modification of MacConkey agar in which lactose has
been replaced with D-sorbitol as the primary
carbohydrate

A

MacConkey sorbitol agar

79
Q

For the selection and differentiation of E. coli

O157:H7 in stool specimens

A

MacConkey sorbitol agar

80
Q

Peptone base, mannitol, and phenol red indicator; salt

concentration of 7.5% inhibits most bacteria

A

Mannitol salt agar

81
Q

Selective differentiation of staphylococci

A

Mannitol salt agar

82
Q

Peptone agar base with cornstarch, supplemented with
yeast dialysate, 3% hemoglobin, and horse plasma;
antibiotic supplement includes vancomycin (2 μg/mL),
colistin (5.5 μg/mL), amphotericin B (1.2 μg/mL), and
trimethoprim (3 μg/mL)

A

New York City (NYC) agar

83
Q

Selective for Neisseria gonorrhoeae;
also supports the growth of Ureaplasma urealyticum
and some Mycoplasma spp.

A

New York City (NYC) agar

84
Q

Nutrient agar base. Phenylmethanol inhibits growth of

gram-negative organisms

A 
Phenylethyl alcohol (PEA)
agar
85
Q

Selective isolation of aerobic gram-positive cocci
and bacilli and anaerobic gram-positive cocci
and negative bacilli

A 
Phenylethyl alcohol (PEA)
agar
86
Q

Charcoal agar supplemented with horse blood, cephalexin,

and amphotericin B

A

Regan Lowe

87
Q

Enrichment and selective medium for isolation of

Bordetella pertussis

A

Regan Lowe

88
Q

Selective for Salmonella and some Shigella spp.

A

Salmonella-Shigella (SS) agar

89
Q

Peptone and soy protein base agar with yeast extract,
dextrose, and buffers; addition of hemin, L-cystine, and
5% blood enriches for anaerobes

A

Schaedler agar

90
Q

Nonselective medium for the recovery of anaerobes
and aerobes
Selective for Campylobacter and Helicobacter spp.

A

Schaedler agar

91
Q

Enrichment of isolation of Salmonella spp.

A

Selenite broth

92
Q

Peptone and soy protein base agar with lysed horse
blood; vancomycin inhibits gram-positive organisms;
polymyxin B and trimethoprim inhibit most gramnegative
organisms

A

Skirrow agar

93
Q

Selective for Campylobacter spp.

A

Skirrow agar

94
Q

Contains crystal violet, colistin, and trimethoprimsulfamethoxazole
in 5% sheep blood agar base

A

Streptococcal selective agar

SSA

95
Q

Selective for Streptococcus pyogenes and

Streptococcus agalactiae

A

Streptococcal selective agar

SSA

96
Q

Selective for Salmonella and Shigella spp. except S.

typhi.

A

Tetrathionate broth

97
Q

Peptone base agar with yeast extract, bile salts, citrate,
sucrose, ferric citrate, and sodium thiosulfate;
bromthymol blue acts as indicator

A

Thiosulfate citrate-bile salts

(TCBS) agar

98
Q

Selective and differential for Vibrio spp.

A

Thiosulfate citrate-bile salts

(TCBS) agar

99
Q

Todd-Hewitt, an enrichment broth for streptococci, is
supplemented with nalidixic acid and gentamicin or
colistin for greater selectivity; thioglycollate and agar
reduce redox potential

A

Todd-Hewitt broth
supplemented with
antibiotics (LIM)

100
Q

Selection and enrichment for Streptococcus

agalactiae in female genital specimens

A

Todd-Hewitt broth
supplemented with
antibiotics (LIM)

101
Q

Isolation and differentiation of Salmonella and
Shigella spp. from other gram-negative enteric
bacilli

A

Xylose lysine desoxycholate

(XLD) agar

102
Q

All-purpose enrichment broth that can support the growth

of many fastidious and nonfastidious bacteria

A

Trypticase soy broth (TSB)

103
Q

Enrichment broth used for subculturing various

bacteria from primary agar plates

A

Trypticase soy broth (TSB)

104
Q

Media sterilization

A

autoclave: 121C, 15 mins
delicate media: membrane filters
with pores size 0.2 - 0.45 μm in diameter

Bacte (2 decks)

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