Media Flashcards
1
Q
Media in a gel or semi-solid form
A
Agar
2
Q
Nutrient agar, trypticase soy agar, nutrient broth
A
General isolation media/ supportive media
3
Q
Sheep blood agar (sba), chocolate agar
A
Nonselective isolation media
4
Q
Trypticase soy agar, Brucella agar, or beef heart infusion
with 5% sheep blood
A
Sheep blood agar
5
Q
Agar is heated or enzyme treated to hemolyzed rbc –> release NAD V factor) and hemin ( X factor)
A
Chocolate agar
6
Q
MacConkey agar, Eosin-Methylene blue (emb)
A
Differential media
7
Q
Peptone base with lactose; gram-positive organisms
inhibited by crystal violet and bile salts; neutral red as
indicator
A
MacConkey agar
8
Q
Peptone base containing lactose; eosin Y and methylene
blue as indicators
A
Eosin-methylene blue (emb)
9
Q
Gram negative broth, selenite broth, tetrathionate broth, thioglycollate broth
A
Enrichment broth
10
Q
Peptone base broth with glucose and mannitol (primary CHO); sodium
citrate and sodium desoxycholate (bile salt) act as inhibitory
agents
A
Gram negative broth
11
Q
Contains sodium hydrogen selenite (toxic for most
Enterobacteriaceae)
Enhance isolation of Salmonella and Shigella
A
Selelnite broth
12
Q
Peptone base broth; iodine and potassium iodide, bile
salts, and sodium thiosulfate inhibit gram-positive
organisms and Enterobacteriaceae
A
Tetrathionate broth
13
Q
Pancreatic digest of casein, soy broth, and glucose enrich
growth of most microorganisms; includes reducing
agents thioglycolate, cystine, and sodium sulfite;
semisolid medium with a low concentration of agar
reducing oxygen diffusion in the medium; yeast and beef extracts, vit K1, hemin, resazurin (redox indicator)
A
Thioglycollate broth
14
Q
Hektoen enteric (he) agar, Salmonella-Shigella (ss) agar, xylose-lysine-deoxycholate (xld) agar
A
Selective media
15
Q
Contains lactose, sucrose, salicin, sodium thiosulfate, ferric ammonium citrate, bromthymol blue and acid fuchsin as indicator
Fermenter cho = yellow-orange
Nonfermenter = green or blue
Hydrogen sulfide producing = black ppt (Salmonella)
A
Hektoen enteric (he) agar
16
Q
Agar
Supports growth of N. gonorrhea and H. Influenzae
A
Chcolate agar
17
Q
Media in liquid form
A
Broth
18
Q
Peptone base with lactose, ferric citrate, and sodium
citrate; neutral red as indicator; inhibition of coliforms
by brilliant green and bile salts
A
Salmonella-Shigella (ss) agar
19
Q
Colistin-nalidixic acid (cna), modified thayer-martin (mtm)
A
Antibiotic media
20
Q
Chocolate base agar, supplement B, vancomycin, colistin, nystatin, trimethoprim lactate Vancomycin = inhibits gram positive Colistin = inhibits gram negative Nystatin = inhibits yeast Trimethoprim = inhibits Proteus
A
Modified thayer martin (mtm)
21
Q
Similar to modified thayer martin (mtm) but anisomycin as yeast inhibitor
A
Martin lewis medium
22
Q
Support the growth of most nonfastidious bacteria
A
General isolation media/ supportive media
23
Q
Contain a nutrient supplement
A
Nonselective isolation media
24
Q
provide distinct colonial appearances of microorganisms to aid in their identification
A
Differential agar
25
```
Peptone base, enriched with solution of 2% hemoglobin
or IsoVitaleX (BBL) -dextrose, cysteine, vit B12, thiamine, ferric nitrate
```
Chocolate agar
26
Supports growth of anaerobes, aerobes (Pseudomonas),
| microaerophilic, and fastidious microorganisms
Thioglycollate broth
27
Isolation and differentiation of lactose fermenting (pink to red)
and non–lactose-fermenting (clear) enteric bacilli (Shigella)
MacConkey Agar
28
In certain bacteria, exceptional media components must be used to grow
Fastidious bacteria
29
Turbidity in broth
A least 10^6 bacteria per mL
30
Type of media
| used to isolate gram negative
Differential media
31
Yeast extract agar with lysine, xylose, lactose, sucrose,
and ferric ammonium citrate; sodium desoxycholate
inhibits gram-positive organisms; phenol red as
indicator
Xylose fermentation
Lysine dacarboxylation
Hydrogen sulfide production
Xylose-lysine-deoxycholate (xld) agar
32
Type of media
| isolation of stool pathogens
Selective media
33
selective inhibit gram positive bacteria and gram negative coliform bacteria
Selective media
34
Selective for N. gonorrhoeae and N. meningitidis.
Supports the growth of Francisella and Brucella
spp.
Modified thayer martin (mtm)
35
Used to inhibit the growth of one organism while enhancing that of another organi
Enrichment broth
36
Process growing microorganisms in culture
Cultivation
37
Used most frequently for stool cultures to inhibit normal flora
Enrichment broth
38
Isolation and differentiation of lactose-fermenting (purple)
| and non–lactose-fermenting (clear) enteric bacilli
Eosin-Methylene blue (emb)
39
Contains sheep blood agar base, colistin (polymyxin e), nalidixic acid
Colistin = disrupt cell membrane of gram negative bacteria
Nalidixic acid = blocks dna replication in gram negative bacteria
Colistin-nalidixic acid (cna)
40
Yeast extract, agar, charcoal, and salts supplemented with
L-cysteine HCl, ferric pyrophosphate, ACES buffer, and
α-ketoglutarate
buffered charcoal-yeast extract agar (BCYE)
41
for selective enrichment of
| group B streptococci
LIM broth
42
nutrient medium for
| fungi
Sabouraud’s dextrose agar
43
selective medium that inhibits the
growth of aerobic and facultatively anaerobic gramnegative
rods and allows gram-positive cocci to grow
phenylethyl alcohol (PEA) agar
44
Nutrient agar base with ferric citrate. Hydrolysis of esculin
by group D streptococci imparts a brown color to
medium; sodium desoxycholate inhibits many bacteria
Bile esculin agar (BEA)
45
Differential isolation and presumptive identification
| of group D streptococci and enterococci
Bile esculin agar (BEA)
46
Contains azide to inhibit gram-negative bacteria,
vancomycin to select for resistant gram-positive
bacteria, and bile esculin to differentiate enterococci
from other vancomycin-resistant bacteria that may
grow
Bile esculin azide agar with
| vancomycin
47
Selective and differential for cultivation of
vancomycin-resistant enterococci from clinical
and surveillance specimens
Bile esculin azide agar with
| vancomycin
48
Potato-glycerol–based medium enriched with 15%-20%
defibrinated blood; contaminants inhibited by
methicillin (final concentration of
2.5 μm/mL)
Bordet-Gengou agar
49
Isolation of Bordetella pertussis and Bordetella
| parapertussis
Bordet-Gengou agar
50
Dextrose, pork brain and heart dehydrated infusions.
Brain heart infusion agar or
| broth
51
Cultivation of fastidious organisms.
Brain heart infusion agar or
| broth
52
Enrichment for Legionella spp.
Supports the growth of Francisella and Nocardia
spp.
```
Buffered charcoal-yeast
extract agar (BCYE)
```
53
BCYE supplemented with polymyxin B, vancomycin, and
ansamycin, to inhibit gram-negative bacteria,
gram-positive bacteria, and yeast, respectively
Buffered charcoal-yeast
extract (BCYE) agar with
antibiotics
54
Enrichment and selection for Legionella spp.
Buffered charcoal-yeast
extract (BCYE) agar with
antibiotics
55
Bile salts, gentamycin, ticarcillin, polymixin B, Peptone,
| yeast extract
Burkholderia cepacia selective
| agar
56
For recovery of B. Cepacia from cystic fibrosis
| patients
Burkholderia cepacia selective
| agar
57
Contains vancomycin (10 mg/L), trimethoprim
(5 mg/L), polymyxin B (2500 U/L), amphotericin B
(2 mg/L), and cephalothin (15 mg/L) in a Brucella agar
base with sheep blood
Campy-blood agar
58
Selective for Campylobacter spp.
Campy-blood agar
59
Thioglycollate broth supplemented with increased agar
| concentration and antibiotics
Campylobacter thioglycollate
| broth
60
Selective holding medium for recovery of
Campylobacter spp.
Incubated at 4° C for cold-enrichment.
Campylobacter thioglycollate
| broth
61
Tryptic soy broth, 5% sheep blood and added nutrients
CDC anaerobe 5% sheep
| blood agar
62
Improved growth of obligate, slow-growing
| anaerobes
CDC anaerobe 5% sheep
| blood agar
63
Blood-supplemented enrichment medium containing
cefoperazone, vancomycin, and amphotericin to inhibit
growth of most gram-negative bacteria, gram-positive
bacteria, and yeast, respectively
Cefoperazone, vancomycin,
amphotericin (CVA)
medium
64
Selective medium for isolation of Campylobacter
| spp.
Cefoperazone, vancomycin,
amphotericin (CVA)
medium
65
Peptone base with yeast extract, mannitol, and bile salts;
supplemented with cefsulodin, irgasan, and novobiocin;
neutral red and crystal violet indicators
Cefsulodin-irgasannovobiocin
| (CIN) agar
66
Selective for Yersinia spp.; may be useful for
| isolation of Aeromonas spp.
Cefsulodin-irgasannovobiocin
| (CIN) agar
67
Cultivation of fastidious microorganisms such as
Haemophilus spp., Brucella spp. and pathogenic
Neisseria spp.
Chocolate agar
68
Organism-specific nutrient base, selective supplements
| and chromogenic substrate
Chromogenic media
69
Chromogenic media are designed to optimize
growth and differentiate a specific type
of organism. Chromagars are routinely used in
the identification of yeasts, methicillin-resistant
Stapylococcus aureus (MRSA), and a variety of
other organisms.
Chromogenic media
70
Columbia agar base with 10 mg colistin per liter, 15 mg
| nalidixic acid per liter, and 5% defibrinated (removed fibrin) sheep blood
Columbia colistin-nalidixic
| acid (CNA) agar
71
Selective isolation of gram-positive cocci
Columbia colistin-nalidixic
| acid (CNA) agar
72
Infusion agar base with 5% sheep blood; reduction of
potassium tellurite by Corynebacterium diphtheriae
produces black colonies
Cystine-tellurite blood agar
73
Isolation of C. diphtheriae
Cystine-tellurite blood agar
74
Selective (enrichment) liquid medium for enteric
| pathogens
Gram-negative broth (GN)
75
Differential, selective medium for the isolation and
differentiation of Salmonella and Shigella spp.
from other gram-negative enteric bacilli
Hektoen enteric (HE) agar
76
```
Animal tissue (heart muscle), dextrose, eggs and beef
serum, and sodium chloride
```
Loeffler’s medium
77
Isolation and growth of Corynebacterium
Loeffler’s medium
78
A modification of MacConkey agar in which lactose has
been replaced with D-sorbitol as the primary
carbohydrate
MacConkey sorbitol agar
79
For the selection and differentiation of E. coli
| O157:H7 in stool specimens
MacConkey sorbitol agar
80
Peptone base, mannitol, and phenol red indicator; salt
| concentration of 7.5% inhibits most bacteria
Mannitol salt agar
81
Selective differentiation of staphylococci
Mannitol salt agar
82
Peptone agar base with cornstarch, supplemented with
yeast dialysate, 3% hemoglobin, and horse plasma;
antibiotic supplement includes vancomycin (2 μg/mL),
colistin (5.5 μg/mL), amphotericin B (1.2 μg/mL), and
trimethoprim (3 μg/mL)
New York City (NYC) agar
83
Selective for Neisseria gonorrhoeae;
also supports the growth of Ureaplasma urealyticum
and some Mycoplasma spp.
New York City (NYC) agar
84
Nutrient agar base. Phenylmethanol inhibits growth of
| gram-negative organisms
```
Phenylethyl alcohol (PEA)
agar
```
85
Selective isolation of aerobic gram-positive cocci
and bacilli and anaerobic gram-positive cocci
and negative bacilli
```
Phenylethyl alcohol (PEA)
agar
```
86
Charcoal agar supplemented with horse blood, cephalexin,
| and amphotericin B
Regan Lowe
87
Enrichment and selective medium for isolation of
| Bordetella pertussis
Regan Lowe
88
Selective for Salmonella and some Shigella spp.
Salmonella-Shigella (SS) agar
89
Peptone and soy protein base agar with yeast extract,
dextrose, and buffers; addition of hemin, L-cystine, and
5% blood enriches for anaerobes
Schaedler agar
90
Nonselective medium for the recovery of anaerobes
and aerobes
Selective for Campylobacter and Helicobacter spp.
Schaedler agar
91
Enrichment of isolation of Salmonella spp.
Selenite broth
92
Peptone and soy protein base agar with lysed horse
blood; vancomycin inhibits gram-positive organisms;
polymyxin B and trimethoprim inhibit most gramnegative
organisms
Skirrow agar
93
Selective for Campylobacter spp.
Skirrow agar
94
Contains crystal violet, colistin, and trimethoprimsulfamethoxazole
in 5% sheep blood agar base
Streptococcal selective agar
| SSA
95
Selective for Streptococcus pyogenes and
| Streptococcus agalactiae
Streptococcal selective agar
| SSA
96
Selective for Salmonella and Shigella spp. except S.
| typhi.
Tetrathionate broth
97
Peptone base agar with yeast extract, bile salts, citrate,
sucrose, ferric citrate, and sodium thiosulfate;
bromthymol blue acts as indicator
Thiosulfate citrate-bile salts
| (TCBS) agar
98
Selective and differential for Vibrio spp.
Thiosulfate citrate-bile salts
| (TCBS) agar
99
Todd-Hewitt, an enrichment broth for streptococci, is
supplemented with nalidixic acid and gentamicin or
colistin for greater selectivity; thioglycollate and agar
reduce redox potential
Todd-Hewitt broth
supplemented with
antibiotics (LIM)
100
Selection and enrichment for Streptococcus
| agalactiae in female genital specimens
Todd-Hewitt broth
supplemented with
antibiotics (LIM)
101
Isolation and differentiation of Salmonella and
Shigella spp. from other gram-negative enteric
bacilli
Xylose lysine desoxycholate
| (XLD) agar
102
All-purpose enrichment broth that can support the growth
| of many fastidious and nonfastidious bacteria
Trypticase soy broth (TSB)
103
Enrichment broth used for subculturing various
| bacteria from primary agar plates
Trypticase soy broth (TSB)
104
Media sterilization
autoclave: 121C, 15 mins
delicate media: membrane filters
with pores size 0.2 - 0.45 μm in diameter
