Mutants

Question 1

How a defect in the mutant strain can be compensated by?

Answer 1

gene product

correct gene

Question 2

What is complementation & trans-complementation?

Answer 2

use of mutant phenotype to screen for a gene in,

same org.

diff. org.

Question 3

what are auxotrophic mutations? How to find them? Explain a procedure where these mutants are used

Answer 3

lacks the ability to synthesize 1 or more growth factors

replica plating

minimal & supplemented medium

Question 4

How can lethal mutations be studied? example?

Answer 4

by isolating conditional mutations

screening is done by replica plating and incubation @ diff. temps

Question 5

What are the diff types of ts mutants? explain each

Answer 5

ts synthesis - proteins made at permis. T are T resistant. not ts in vitro

ts activity - protein is ts regardless of it was synth at permis. or non-permis. T. also ts in invitro