Hybrid Vectors Flashcards
1
Q
Why hybrid vectors are made?
A
to overcome the disadv. of plasmid & bacteriophage vectors
2
Q
List 2 types of hybrid vectors and their components and properties
A
phagemids
plasmid ori for ds replication
cosmids
3
Q
Describe the steps in cosmid cloning process
A
ss
4
Q
Why artificial vectors are used? examples?
A
bcos even hybrids have size limitations
5
Q
BAC characteristics? based on what? disadvantages?
A
have elements of chromosomal replication
can hold >300kb
1-2 copies per cell
based on F factor
low copy number
6
Q
BAC structure? How to clone using BAC?
A
ss
7
Q
Describe how cloning is done using YAC?
A
slides
