Multiplex PCR Flashcards
Polymerase ____ _____ permits more than ____ region of DNA to be ____ _______ by adding _____ primer sets to the reaction ______
Chain Reaction One Copied Simultaneously Multiple Mixture
Primer pairs need to be _____ for a multiplex reaction to work
Compatible
Multiplex __ makes use of ____ or ____ primer sets in the ____ reaction mixture
PCR
Two
More
Same
Single Locus Probes (3)
1) Target one locus at a time
2) If additional information needed, you would need to re analyse the sample
3) No limit to number of loci investigated but means ENTIRE re analysis per loci
When was the QUAD multiplex invented, who by and how many STR loci could it test for?
1994 by the FSS
4 STR loci
What is the MPr of the Quad multiplex?
1 in 40,000 for full profiles
what does SGM stand for?
Second Generation Multiplex
When was SGM developed and who by?
1995 by the FSS
How many STR loci does SGM test for?
6 STR Loci
Whats the MPr for SGM
1 in 50 million
SGM incorporated the _____ test for _____
Amelogenin
Gender
___ became the technique that the ____ was established
SGM
NDNAD
What problem emerged with SGM after time?
The NDNAD grew too large for SGMS level of discrimination to sufficiently rule out chance matches of profiles obtained from crime scenes or profiles of individuals unconnected with the offence.
How many STR loci could SGM plus test for and what is the breakdown of these loci?
10 (Technically 11)
6 STR from SGM
4 New STR loci
+ Amelogenin
What is the MPr of SGM Plus?
1 in 1000 million
