multi complex disassembly linked Flashcards

1
Q

what are WNT receptors

A

7 transmembrane receptors but not G protein coupled receptors

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2
Q

how does WNT cause changes in transcription

A

it activates the TCF transcription factor

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3
Q

in the absence of WNT, what is beta catenin held in a complex with

A

beta catenin- axin- GSK3- APC

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4
Q

what is the role of GSK3

A

it phosphorylates beta catenin which degrades it and stops it binding to TCF

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5
Q

another name for WNT receptors

A

frazzled (FZ) receptor

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6
Q

what happens with WNT binds to its receptor

A

GSK3 phosphorylates LRP

this permits axin binding to LRP

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7
Q

what does actin binding to LRP cause

A

complex disassembly, beta catenin is no longer phosphorylated
it can move to the nucleus and bind TCF

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8
Q

what is the purpose of Hh (hedgehog) signalling

A

transmits info to embryonic cells for differentiation

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9
Q

where is Smo kept when Hh is absent

A

in internal vesicles guarded by Ptc (patched receptor)

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10
Q

where is Ci kept when Hh is absent

A

in a microtubule bound complex guarded by Cos2 and Fu

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11
Q

what phosphorylates Ci

A

PKA,CK1, GSK3

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12
Q

what degrades phosphorylated Ci and what happens after

A

degraded by Slimb into Ci75 which travels to nucleus to prevent transcription

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13
Q

what happens to Smo when Hh is present

A

Hh binds to Ptc which lets Smo escape to the plasma membrane where it is phosphorylated by PKA and CK1

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14
Q

what happens to the complex when Hh is present

A

Cos2 and Fu are phosphorylated and form a complex with Smo

Ci escapes from the disassembled complex

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15
Q

how does Ci mediate transcription

A

it moves to the nucleus and forms a complex with CRB to mediate transcription

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