MTAP 2 Bloodbanking

Question 1

Enumerate the Factors that Affect Sensitization of RBCs

Answer 1

1. Ag:Ab ratio
2. Temperature
3. Incubation Time
4. pH
5. size of the antibody
6. Site of Antigen
7. Zeta potential

Question 2

Enumerate the Ways to lessen the Zeta Potential

Answer 2

1. LISS
2. Enzymes
3. 22% Bovine albumin
4. Polyethylene Glycol (PEG)

Question 3

Temperature for IgM

Answer 3

Cold (4-20C)

Question 4

Temerature for IgG

Answer 4

37C

Question 5

Products of anaerobic glycolysis in RBCs

Answer 5

Lactic acid + ATP

Question 6

The correct ratio of Ag:Ab for sensitization

Answer 6

1 part 5% Red cells: 2 parts Anti-sera/
1Ag: 2Ab

Question 7

Prozone phenomenon

Answer 7

more ANTIBODIES than antigen

Question 8

Post-zone phenomenon

Answer 8

More ANTIGENS than antibodies

Question 9

What is the glucose transporter in RBC membrane called?

Answer 9

GLUT-1

Question 10

The only pathway that supplies the red cells with ATP

Answer 10

Glycolysis

Question 11

What happens when 2,3-DPG is activated?

Answer 11

-Shft to the right
-Forms anionic salt brifge to release oxygen
-Increase release of oxygen to peripheral tissues

Question 12

Which pathway maintains iron in a reduced state for effective transport of oxygen

Answer 12

Methemoglobin Reductase Pathway

Question 13

Glutathione is needed in reduced form for:

Answer 13

1. Elimination of peroxide
2. Protection of proteins GH groups
3. Provides ribose-5-phosphate needed for PRPP