MRL

Question 1

Blot\line assay is confirmatory test for :

Answer 1

HIV

HCV.

Question 2

Main tasks for MRL

Answer 2

1-confirmation
2-evaluation and validation
3-preparation of q.c for screening assay

Question 3

How to calculate the ratio of sample in ELISA run

Answer 3

Ratio = optical density of SAMPLE/CUT OFF

Question 4

How to deal with Q.C ratios outside L.C.L and U.C.L.

Answer 4

1- Reject this Q.C reading and reject all the run
2- Retest all samples again
3- Investigate for samples and procedure:
a) Sample : check for the expiry date and the method of trasportation & storage
b) Procedure : - must follow SOPS - All staff must be well trained on this SOPS
- All equipment used in this procedure must be well maintained and calibrated

Question 5

How many times is accepted of thawing and freezing of frozen Aliquots

Answer 5

2- 3 times

Question 6

The Key factors of the basic criteria of evaluation and validation of test kits

Answer 6

Sensitivity

Specificity

Question 7

All are Criteria of the sample reception in MRL Except

Answer 7

Volume

Not lipemic

Not hemolyzed

Except

colour of the test tube

Question 8

What is 2ry confirmatory test for syphilis?

Answer 8

TPHA.

Question 9

What is 2ry confirmatory test for HbsAg ?

Answer 9

Neutralization.

Question 10

What is 2ry confirmatory test for HCV ?

Answer 10

Blot\line assay.