Movement of substances into and out of cells - Paper 1 Flashcards
Define equilibrium
Particles will move to reach equilibrium- both sides of the membrane have an equal concentration or number of particles
What is osmosis
- Osmosis is the movement of water across a semi permeable membrane (a membrane which only allows some particles through)
- it is a process by which molecules of a solvent tends to pass through a semipermeable membrane from a less concentrated to a more concentrated solution
- at first, the concentration of solute will be very high on one side of the membrane but, over time, the water will move across the semipermeable membrane and dilute the particles on this side- making both sides of the membrane reach equilibrium.
- from high concentration (of water) to low concentration (of water)
- from (an area of) dilute solution to an area of concentrated solution
Draw a diagram of osmosis
Include:
- outside of cell
- inside of cell
- semi permeable membrane
- high/low water concentration
- direction of movement
What is a turgid cell?
- occurs when a plant cell is in a hypotonic solution
- water enters by osmosis and the vacuole swells and pushes against the cell wall
What is a flaccid cell?
- occurs when a plant cell is in a hypertonic solution
- water is lost from the cell and the vacuole shrinks so the cell loses shape
What is a hypertonic solution?
- a solution containing less water than the cells inside
- If a cell is placed in a hypertonic solution, water will leave the cell, and the cell will shrink
What is an isotonic solution?
- a solution containing the same amount of water as the cells in it
- there is no net water movement, so there is no change in the size of a cell put into it
What is a hypotonic solution?
- a solution with more water in it than the cells within
- When a cell is placed in a hypotonic environment, water will enter the cell, and the cell will swell, sometimes burst
Describe the experiment used to investigate the effect of concentration of blackcurrant squash on osmosis in chipped potatoes
-label beakers with different concentrations of the squash you will use
-prepare different concentrations of the squash, each 30cm3 (concentrations from 0-100%)
-peel, blot and cut the potatoes, making sure each slice has the same mass
-place the chips into the squashes, wait 10 mins, then record the mass of the final potato chips
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Why is it a good idea to remove all the potato skin in the potato blackcurrant squash experiment?
- The potato skin may slow the rate of diffusion between the squash and potato chip
- when you peel the potatoes the water particles don’t need to diffuse through any skin.
Why do we dry the chips on a paper towel before weighing them in the potatoe blackcurrant squash experiment
So that any water on the chips is not included in the overall weight of the chip which would give an accurate results
Explain in biological terms what is happening to the potato chips in the different blackcurrant squash solutions
- when the concentration of the squash is between zero and 20% the chip increases in mass
- this is because the black current squash has a higher concentration of water than the potato cells so the water molecules diffuse into the cells causing the chip to become larger and heavier
- when the squash has concentrations over 28% the mass of the chip decreases as the chip contains a higher concentration of water particles therefore will diffuse water particles into the squash
What is the equation used to calculate percentage change
Percentage change= (change in mass/initial mass) x100
Describe the experiment used to investigate osmosis in Dandelion stems
- Cut Dandelion stems into sections and then strips about 5 cm long and 3 mm wide using a scalpel and ruler
- prepare five beakers with different concentrations of sodium chloride solution and one with only water using a measuring cylinder
- place each Dandelion strip into one beaker and after 10 minutes record the results and repeat for accuracy
What are the variables for the experiment investigating osmosis in Dandelion stems
Independent: concentration of sodium chloride
Dependant: the way the stems curl
Controls: size of dandelion strips, time for the stems to soak, volume of liquids, volume of water in the stalks (preserve in water)