MOUNTING/LABELING Flashcards
last step on the tissue processing
Mounting
syrupy fluid applied between the section and the coverslip IMMEDIATELY after staining
Mounting medium
Mounting a tissue specimen is essential for preserving the specimen during ______ (for long periods of time) as well as for enhancing _____ quality during microscopy
storage
image
Histopath slides → can last up to _____ years retention period
10
Mounting medium:
[Action] tissue section. Not ____ or blurred
flatten
wavy
blurred
If an unmounted stained section is seen in the
microscope, differences in ______ between the glass slides and the tissue components, may lead to difference in _________; hence, very little microscopic detail can usually be appreciated.
◆ Some parts of tissue sections are [2]
light refraction
length dispersion
blurry/wavy
We need to use _______ to secure properly the glass slide and to prevent movement and washing out or scratches
mounting medium
Mounting medium flatten out sections for what purpose?
fine details of cells
Mounting medium purposes [4]
promote spx adhesion
promote glass slide adhesion
prevent spx movement
spx preserve
Characteristics of a Good Mounting medium
- freely miscible w/ xylene/toluene
- should not dry quickly
- should not dissolve/fade out tissue sections
- should not crack or produce artefactual granularity on the slide upon drying
Dryness will cause _____ on glass slides, resulting to the tendency of tissue section to be destroyed.
cracks
artefactual granularity causes ?
bubbles
No bubbles or cracks must be produced
because you will not appreciate the cells’ [2]
nucleus
cytoplasm
Ratio of speed of light in air and in a specific medium
Refractive Index
Value to which light can pass through a
medium
RI
Refractive index should be as near as possible to
that of the glass which is [#]
1.518
As much as possible the RI must be higher
than the RI of ____ to consider that the mounting medium is of good quality
glass
________
They are designed to mount water miscible preparations directly from water where the stained is removed or decolorized with ____ or _____.
Because there are components of tissue slides that are dissolved when using alcohol or xylene: ______
Aqueous Media
alcohol
xylene
lipids
Aqueous Media is made up of? [4] [+function]
Gelatin, Glycerin Jelly or Gum Arabic- solidifigcation
Glycerol- prevent cracking/drying
Sugar- increase RI
Merthiolate- preservative
Aqueous Media examples [6]
Water
Mineral Oil
Glycerin
Levulose
Brun’s Fluid
Farrant’s medium
Von Apathy’s Gum Syrup
Karo Corn Syrup
Has low RI
➔ Moderately transparent
➔ Evaporates easily
Water
➔ Good only for temporary mounting
Water
May be used also as preservative for Aqueous Media other than merthiolate
Glycerin
➔ Has high refractive index, RI 1.46
➔ Last for a few minutes
Glycerin
Glycerin Jelly RI:
1.47
Farrant’s medium RI:
1.43
Useful temporary mounting media for Romanowsky stained smear
Mineral Oil
Von Apathy’s Gum Syrup RI:
1.52
Von Apathy’s Gum Syrup is made up of? [5]
Distilled water
Sucrose
Pure gum arabic
Pure cane sugar
Thymol