Motor proteins Flashcards
Dynein;
o Structure of the motor proteins, including alternate forms (eg. myosin II vs V)
two heavy chains and smaller intermediate/light chains at base. Large, globular, force-generating head; stalk w/ binding site for MT and a stem.
Kinesin 1
tetramer,2 heavy/light chains. Pair of heads that bind at MT connected to neck, stalk, and fan shaped tail.
Myosin 2 structure
Head binding actin filament and binding/hydrolyzing ATP. 1 pair heavy chains, 2 pairs light, pair of alpha helix necks w/ 2 light chains, tail of heavy chains interwoven
Myosin
o The associated cytoskeletal proteins
Actin
Myosin
o Any directionality associated with the motor protein? Be specific
Type 2 moves to barbed end of actin filament
Myosin
o What is accomplished by their movement?
* NOTE: The videos uploaded to Canvas’ Media Gallery, as well as others can be helpful to visualize
the processes of movement
Creates movement by turning chemical energy released from ATP into mechanical energy, cell migration, and the turning behavior of growth cones
Kinesins
o Their method of movement along their respective cytoskeletal proteins
- One head binds to the microtubule,
- Conformational changes in neck region make other head to move forward toward the next binding site on the protofilament.
- The forward-moving head binds to site randomly.
Kinesins
o Any directionality associated with the motor protein? Be specific
+ end
Kinesins
o What is accomplished by their movement?
* NOTE: The videos uploaded to Canvas’ Media Gallery, as well as others can be helpful to visualize
the processes of movement
Cell division, cargo transport
Dynein
o Any directionality associated with the motor protein? Be specific
Retrograde (-) end
Dynein
o What is accomplished by their movement?
* NOTE: The videos uploaded to Canvas’ Media Gallery, as well as others can be helpful to visualize
the processes of movement
Cilia and flagella movement
Difference between kinesin 1 and kinesin 14
+ end directed molecule on kinesin 14
Myosin 5 structure (unconventional)
One head
