Monoclonal Antibodies Flashcards
What are monoclonal antibodies
Monoclonal antibodies are proteins. If all the protein s come from one cell they are monoclonal.
What is stage 1 in the making of monoclonal antibodies?
.mouse lymphocytes are stimulated to make a particulare antibody.
What is stage 2 in the making of monoclonal antibodies
Scientists combine a mouse lymphocyte with a tumour cell to make a hybridoma.
What is stage 3 in the making of monoclonal antibodies?
.single hybridoma cells divide to make a large number of identical cells that all produce the same antibody.
What is stage 4 of making monoclonal antibodies?
.the antibodies from the hybridoma cell dividing are collected and purified.
How do pregnancy tests use monoclonal antibodies?
Pregnancy tests rely on monoclonal antibodies that attached hormones found in early pregnancy.
How can monoclonal antibodies be used to diagnose disease?
Specific antibodies can attach to antigens on a pathogen, or some types of cancerous cells.
How can scientists track down specific molecules in a cell?
By attaching flourescent markers to a monoclonal antibody