Monoamine Transporters Flashcards
What is LSD’s half life and how long does its effects last for?
175 minute half life and effects last for 9-12 hours
What are the effects of LSD?
Hallucinations, euphoria, pupil dilation, loss of appetite and wakefulness
What is the half life of psilobycin and how long do its effects last?
2 hour half life and effects last for 4-5 hours
What are the effects of psilocybin?
Euphoria, hallucinations, ‘connectedness’, nausea, panic attacks
Monoamine transporters are involved in the mechanism of action of what drugs?
MDMA, cocaine, amphetamine and ritalin
What is the half life of MDMA?
8 hours
What are the effects of MDMA?
increased wakefulness and energy, euphoria, increased empathy and sociability, heightened sense, bruxism (teeth grinding), increased heart rate and blood pressure, increased temperature, anxiety, hyperactivity and insomnia.
What are two examples of in vitro assays that can be used to show how drugs are metabolised?
Ligand binding assay - radio labelled compound is used, wash through filter with membrane bound receptor, measure residual activity
Competitive binding assay - Measure how much radio labelled compound A displaces unlabelled B
What are the advantages and disadvantages for in vitro drug activity methods?
Advantages = Cheap, reproducible, high throughput, possible to study single proteins or receptors in isolation, no testing on animals Disadvantages = Unrealistic, not possible to measure metabolites, limited pharmacokinetic data.
What is in vivo drug activity methods?
Measuring the effect of a compound in a whole animal setting .g. in vivo micro dialysis
What are the advantages and disadvantages of in vivo drug activity methods?
Advantages - allows measurement of effects without fully understanding the mechanism, cam measure drug and active metabolites, can measure pharmacokinetics and is more physiologically relevant than in vitro.
Disadvantages - Using whole animal (ethically questionable and costly), low throughput, can be difficult to determine mechanisms, negative results are difficult to determine/interpret.
