Monday Diebs

Question 1

difference between selective and differential media

Answer 1

Selective Media
Contains compounds that selectively inhibit growth of some microbes but not others

Differential Media
Contains an indicator, usually a dye, that detects particular chemical reactions occurring during growth

Question 2

what common bacterial are catalase positive

Answer 2

STAPH

Question 3

What common coccus bacteria are coagulase positive? coagulase negative?

what actually does the coagulating?

Answer 3

staph aureus - coagulase positive

staph saprophyticus - coagulase negative

Bound coagulase (the type of coagulase in the bacterial cell wall) binds to fibrinogen in plasma and clumps up the bacterial cells.

free coagulase is an extracellular enzyme that causes formation of clots

Question 4

when would you use a novobiocin sensitivity test

what does novobiocin do

Answer 4

to differentiate between:

Staphylococcus saprophyticus (resistant)

Staphylococcus epidermidis (sensitive).

novobiocin inhibits bacterial DNA gyrase

NO StRESs

NOvobiocin—Saprophyticus is Resistant;
Epidermidis is Sensitive.

Question 5

first line treatment for S. sapro

Answer 5

augmentin

Question 6

E coli is

oxidase _____
indole _____

(negative or positive?)

Answer 6

oxidase-negative

indole positive

Question 7

things in the Enterobacteriaceae family

Answer 7

salmonella, e. coli, klebsiella, proteus, enterobacter to name a few

gram negative

Question 8

type 1 pilus

Answer 8

binds uroplakins and initiates infection

all e coli have it but not all express it

Question 9

p fimbriae

Answer 9

causes infections to move up from the bladder into the kidney