Molecule of heredity - Lecture 1

Question 1

What was the Griffith transformation experiment?

Answer 1

The effects of smooth and rough bacteria on mice

Question 2

What happened to the mouse if it was infected with live smooth bacteria?

Answer 2

Dead

Question 3

What happened to the mouse if it was infected with head-killed smooth bacteria?

Answer 3

Alive

Question 4

What happened to the mouse if it was infected with live rough bacteria?

Answer 4

Alive

Question 5

What happened to the mouse if it was infected with heat-killed smooth bacteria and live rough bacteria?

Answer 5

○ Dead
○ Live smooth bacteria was recovered

Question 6

How was the Avery-McCarty-Macleod experiment done?

Answer 6

○ Make extract from smooth infective bacteria
○ Add different enzymes to it and mix the extract with live rough bacteria

Question 7

What happened to the mouse if protease had been added?

Answer 7

Dead

Question 8

What happened to the mouse if RNase had been added?

Answer 8

Dead

Question 9

What happened to the mouse if DNAase has been added?.

Answer 9

Alive

Question 10

What is a bacteriophage?

Answer 10

○ Virus that infects bacteria
○ A protein coat surrounding a nucleic acid core

Question 11

What was the Hershey and Chase experiment?

Answer 11

○ Infect bacteria with phage
○ Use radioactive labels (32P for DNA and 35S for protein)

Question 12

What happened when the phage coats and infected bacteria was separated?

Answer 12

○ Phage mostly has 35S label
○ Infected bacteria mostly has 32P label

Question 13

What did the progeny phage contain?

Answer 13

○ 30% of 32P label
○ <1% of 35S label

Question 14

What did the Hershey and Chase experiment show?

Answer 14

○ DNA, not the protein, enters the cell
○ DNA of the parent phages becomes part of the progeny phage