Molecular Techniques

Question 0

How is it that restriction endonucleases don’t attack themselves?

Answer 0

methylation

Question 1

What is the difference between an endonuclease and an exonuclease?

Answer 1

Exonuclease cuts at the end of the DNA. Endonuclease cuts within the DNA.

Question 2

What is a common restriction enzyme?

Answer 2

EcoR1 and GAATTC and HindIII

Question 3

Sticky ends are _______

Answer 3

Palindromic

Question 4

Sticky ends can be used to ___

Answer 4

Combine DNA from different organisms

Question 5

PCR can be used to

Answer 5

amplify DNA of interest

Question 6

What is the organism that provides the DNA polymerase to do PCR at hot temperatures?

Answer 6

Thermus Aquaticus

Question 7

Genomic PCR is used to _____

Answer 7

amplify DNA

Question 8

Reverse Transcriptase PCR (RTPCR) is used to find out what is _____

Answer 8

expressed

Question 9

PCR products cut with restriction enzymes can be used to find ____

Answer 9

Restriction Fragment Length Polymorphisms (RFLPs)

Question 10

RFLPs can be used as a _____

Answer 10

diagnostic tool

Question 11

dideoxynucleotides can be thought of as

Answer 11

defective nucleotides

Question 12

Point mutations can cause new sites for

Answer 12

restriction endonucleases to cut DNA

Question 13

Small DNA runs faster or slower?

Answer 13

faster

Question 14

A single mutation resulting in a cut site could produce __ DNA stripes on a blot?

Answer 14

2

Question 15

2 mutations in DNA could result in ___ DNA stripes on the blot?

Answer 15

3

Question 16

Multiple SNPs can demonstrate associations in

Answer 16

Drug Response
Disease Prognosis
Disease Occurrence
Age of onset

Question 17

Southern Blotting is a technique used to _____

Answer 17

view DNA of interest