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BIO 4U > molecular genetics > Flashcards

molecular genetics Flashcards

1
Q

nucleic acid

A
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2
Q

nucelotides (draw)

A
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3
Q

building nucleaic acids

A
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4
Q

difference sbetween dna and rna

A
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5
Q

dna replication:

A

semiconservative

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6
Q

requirments for dna replication

A
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7
Q

steps of dna replication

A
  1. helicase unzips souble helix
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8
Q

the enzymes involved in dna replication

A

helicase, gyrase ssb
sbna primase
dna polymerase 3
dna polymerase 2
ligase

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9
Q

the centra dogma

A
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10
Q

transcription: types of rna and where theyre found

A
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11
Q

requirements for transcription

A
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12
Q

steps of transcription

A
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13
Q

enzymes involved in transcriotuon

A
  • rna polymerase
    transcription factors
    splicesomes
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14
Q

where does replication, transcripton, and translation happen

A

rep: in nucleus
transcription: in nucleus
translation: ribosomes

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15
Q

translation

A
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16
Q

requirements for translation

A
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17
Q

enzymes of translations

A
  • small and large ribosomal subunit
  • aminoacylt-t rna synthetase
  • relaese factors
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18
Q

steps of translation

A
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19
Q

ribosomes

A
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20
Q

function of trna

21
Q

start codon

22
Q

immature mrna and post transcriptional eiting

23
Q

types of mutations

A

point and chromosomal

24
Q

silent mutations

A

missesne mutations

25
Q

nonsense mutations

26
Q

deletion mutations

27
Q

insertion mutations

28
Q

translotcation mutatins

29
Q

inversion mutations

30
Q

gene regulation

31
Q

lac operon and how it works

32
Q

lac operon ON

33
Q

lac operon OFF

34
Q

lacz lacy laca

35
Q

trp operon

36
Q

trp ON

37
Q

trp OFF

38
Q

eukaryotic gene expression transcription level a) transcriptional

39
Q

eukaryotic gene expression transcription level b) post transcriptional

40
Q

restriction endonucleases

41
Q

restriction enzymes

42
Q

sticky ends

43
Q

blunt ends

45
Q

GEL electrophoreisis (steps and uses)

46
Q

polymerase chain reaction

47
Q

taq polymerase

47
Q

transformation

A

(heatshock and electroporation)

BIO 4U (3 decks)

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