Molecular Diagnostics Tutorial Flashcards
what is the appropriate way to take a sample from a child
swab of saliva or tooth surface
what organisms are important to detect in caries
strep mutans
lactobacillus species
what methods of isolating bacteria can be used
next generation sequencing
microbiome analysis of 16S rRNA
would knowing which bacteria and carriage levels of these bacteria impact overall levels of caries
yes because you can use it to monitor changes over time and can lead to change in public health measures
what preventive measures can be used to minimise bacterial carriage for caries
improved diet advice
behaviour advice
public health measures - fluoride
what specimens do you investigate for periodontal disease
subgingival plaque biofilm
where do you take periodontal disease microorganisms from
paper point from gingival crevice on site of inflammation
what organisms are important for periodontitis
p gingivalis
AA
what methods can be used to identify the culprit organism in periodontal disease
selective agars
PCR
NGS
grow on plates and undertake sensitivity testing
how do you determine cause and effect of periodontitis
removal of culprit organism leads to resolution of disease
what treatment options are available for periodontal disease
oral hygiene
antiseptics
antibiotics if unresponsive
what is the cause of inflammation associated with a denture
denture associated biofilm
what microorganisms are associated with denture stomatitis
candida albicans
candida glabrata
what types of specimens can be taken to isolate microorganisms associated with denture stomatitis
oral rinse or swab from localised area
why is it important to identify candidal species
c glabrata is insensitive to azoles
what are the systemic implications of an indwelling prosthesis for patients
aspiration pneumonia
what are the microbiologically concerns for a patient with a facial swelling
are they systemically unwell?
what type of clinical specimen should you take for someone with an abscess
take a pus sample so need a needle to aspirate it
what type of microorganisms are you looking for in an abscess
oral anaerobes
if you have an anaerobic species how would you take the specimen
be weary as they are oxygen sensitive so need anaerobic transport media
what techniques are useful to identify microorganisms
standard plate culture and microscopy
what are the systemic implications of an abscess if not dealt with
spreading odontogenic infections
possibility of sepsis
