Molecular Diagnostics Flashcards
How is Equine Influenza detected?
Virus isolation or detection from nasal or nasopharyngeal swabs collected from horses in the early febrile stage of the disease
How are EHV-1 and EHV-4 resp dz detected?
Virus isolation of nasal or nasopharyngeal swab or citrated or heparinized whole blood. PCR of respiratory secretions, peripheral blood lymphocytes, and other tissues
How is Streptococcus equi equi detected?
Culture or PCR of nasal swab or guttural pouch lavage.
PCR three times more sensitive than culture.
How is S. equi zooepidemicus detected?
Culture or qPCR from a draining lymph node or guttural pouch empyema
How is Rhodococcus equi detected?
Culture of TTW - gold standard. Detection of the vapA gene through PCR of TTW.
Detection of EPM?
Detection at necropsy - gold standard. Serologic assays in serum and CSF.
Detection of WNV?
Isolation of WNV from blood, CSF or tissue; a four fold increase in plaque reduction neutralization test antibody titers on paired serum samples taken 2 weeks apart; detection of IgM antibody to WNV by IgM capture ELISA
Detection of EHV-1 myeloencephalopathy?
History, clinical signs, xantochromia, and elevated total protein concentration in CSF.
Detection of Borrelia burgdorefi?
qPCR specific, but results are often negative due to the rare occurrence of the spirochetes in the CSF
Detection of Neorickettsia risticii?
Blood and feces PCR
Detection of Lawsonia intracellularis?
Serology and PCR
Detection of Salmonella?
PCR and culture
Detection of equine Coronavirus?
PCR
Detection of Rotavirus?
PCR
Detection of Clostridium dificille?
Detection of toxins A and B in feces, gastric reflux, or colonic content. ELISA or PCR
