Collection and Analysis of Bone Marrow Flashcards
What is the bone marrow composed of?
Hematopoietic cells as well as structural components, including trabecular bone, adipose tissue, a sinusoidal system, supporting reticular cells, and an extracellular matrix
Why perform a bone marrow evaluation?
- to evaluate cytopenias
- observation of atypical cells, unexplained immature cells, or abnormal blood cell morphology on a peripheral blood film
What to do if you have a poorly representative sample ?
Get a core biopsy
Why is assessing iron stores useful when evaluating anemia?
- Lack of iron stores - iron deficiency
- Abundant iron stores - anemia of chronic disease
If you are not seeing megakaryocytes frequently in a thrombocytopenia animal, what does it mean?
Suggests hypoplasia or destruction of megakaryocytes
What does apparent maturation arrest mean?
Apparent maturation arrest occurs when maturation appears to terminate at a particular stage
When do you see apparent maturation arrest?
- Immune-mediated disease
- Large demand or rapid mobilization of cells out of the bone marrow
- Acute leukemia
What is left shift?
All maturation stages are represented but there is a higher proportion of less mature cells
Why is the M/E ratio helpful?
Helps determine if the erythrocyte or myeloid cell lines are hypoplastic or hyper plastic. An equal number of nucleated erythroid and myeloid cells will result in a M/E ration of 1. Check the CBC!!!
What does an M/E greater than 1 indicates?
- Granulocytic production exceeds erythroid production
- Decrease in erythropoiesis
What does an M/E less than 1 indicates?
- Erythroid production exceeds granulocytic production
- Suppression of granulocytic production
What can atypical cell populations in the bone marrow represent?
Myeloproliferative disorder, infiltrative neoplastic process, inflammation, or antigenic stimulation
