molecular diagnostics

Question 1

What do you need to know in order to run hybridization or PCR?

Answer 1

know the sequence of the pathogen

Question 2

what is hybridization used for?

Answer 2

detect and quantify target DNA;
works with DNA or RNA;
then hybridized via southern or northern blotting

Question 3

What is a southern blot?

Answer 3

DNA

Question 4

northern blot

Answer 4

RNA

Question 5

western blot

Answer 5

protein or antibody

Question 6

eastern blot

Answer 6

post-translational modifications

Question 7

Describe hybridization

Answer 7

you create a flagged primer, if DNA or RNA is present in the sample then it will bind, then you run it through a gel to separate different desities, then transfer to a membrane

Question 8

describe pcr

Answer 8

Denature DNA in sample, have primers that attach to each DNA strand and then add dNTPs
Taq polymerase
if you have small sample of DNA then you can amplify it

Question 9

describe quantitative pcr

Answer 9

used to compare two samples’ (find amount of specific genes);
have to use a probe that lights up when certain sequence is reached?

Question 10

what is rflp?

Answer 10

forensic analysis;
dna fingerprinting based on differences in DNA;
DNA cleaved and run through gel and compare band travel

Question 11

what is vntr?

Answer 11

Variable number tandem repeats;

PCR, gel, then compare band location

Question 12

describe ELISA

Answer 12

detect antigen or antibody concentration;

antibodies are at the bed of the well, add the sample and then wash it, add a flag antibody to observe

Question 13

what is indirect ELISA?

Answer 13

detect amount of antibody in sample

Question 14

What is Sandwhich ELISA?

Answer 14

detect the amount of antigen in a sample