Molecular diagnosis Flashcards
pcr, autoradiography ELISA
1
Q
What technologies serve the purpose for early diagnosis?
A
1) PCR ( Polymerase chain reaction)
2) ELISA ( enzyme Linked Immuno Sorbent Assay)
2
Q
How can we detect a very low conc. of bacteria or virus?
A
by amplification of their Nucleic acid by PCR
3
Q
How many times can nucleic acid be amplified by a PCR ?
A
upto a billion times in 30 cycles.
4
Q
PCR can detect very low
amounts of_____ ?
A
DNA
5
Q
PCR is routinely used for the detection of ?
A
HIV in AIDS patients
6
Q
What does Auto radiography show us ?
A
that the distribution and localization of radio active compounds in the body
7
Q
On what principle ELISA is based on?
A
based on the principle of “ antigen - antibody “ interaction
