Biotechnological applications in medicine 2 Flashcards

Structure of insulin,gene therapy

1
Q

Insulin consists of two short polypeptide chains namely?

A

A and B linked together by disulphide bonds

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2
Q

In which form is insulin synthesized in humans ?

A

as a pro - hormone

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3
Q

What is the extra stretch in insulin called?

A

C peptide

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4
Q

what is the main challenge for the production of insulin using rDNA techniques?

A

getting insulin assembled in a mature form

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5
Q

Which American company prepared two dna sequences of human insulin ?

A

Eli Lilly , 1983

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6
Q

Where was this insulin introduced into ?

A

in plasmids of E.coli

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7
Q

Which other human hormone can be made by genetic engg.

A

somatotropin (growth hormone )

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8
Q

Define Gene Therapy

A

it is a collection of methods that allows correction of a gene defect that has been diagnosed in a child/embryo.

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9
Q

When was the first clinical gene therapy given

A

1990

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10
Q

To whom was the first gene therapy given

A

4 year old girl with Adenosine deaminase deficiency (ADA)

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11
Q

How is ADA caused?

A

caused due to the deletion of the gene for ADA which causes SCID

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12
Q

SCID stands for ?

A

Severe Combined Immuno Deficiency

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13
Q

How can ADA be cured ?

A

1) bone marrow transplantation
2) enzyme replacement therapy

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14
Q

Permanent cure for ADA

A

If the gene isolates from marrow cells producing ADA is introduced in the cells at early embryonic stage

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