Molecular Biology Techniques Flashcards
def. Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR) is a technique that allows amplification (making many copies) of a specified segment of DNA in vitro.
def. Gel eletrophoresis
Gel electrophoresis is a technique that separates mixture of DNA, RNA or proteins according to their molecular size.
def. Agarose
Agarose is a polysaccharide derived from seaweed that forms a gel when dissolved in a heated aqueous solution.
def. Southern blotting
Southern blotting, which is typically carried out after gel electrophoresis, is a technique used to detect and confirm fragments containing specific nucleotide sequences within a sample of DNA.
def. DNA polymorphism
DNA polymorphism refers to differences in DNA sequences in homologous regions among different individuals.
def. Restriction Fragment Length Polymorphism (RFLP)
Restriction Fragment Length Polymorphism (RFLP) refers to unique banding pattern among individuals when their genomic DNA is digested by restriction enzymes, separated by gel electrophoresis and subjected to Southern hybridisation.
Electrophoresis
Electrophoresis is movement of charged molecules in an electric field.
