Molecular Biology of the Cell

Question 1

What is the aim of this practical?

Answer 1

Identifying mutations that alter fluorescence in transgenic bacteria

Question 2

What causes are we investigating?

Answer 2

investigating the causes for the loss of fluorescence in four different cultures

Question 3

What did we do in practical 1?

Answer 3

Test if any of the cultures have lost their ability to fluoresce

Question 4

What did we do in practical 2?

Answer 4

determine the nature of the GFP mutation by sanger sequencing the plasmid DNA

Question 5

What did we do in practical 3?

Answer 5

use western blotting to evaluate the eGFP production (protein amounts and size) in the four cultures and see if the mutations had any consequence on this

Question 6

What did we do in practical 4?

Answer 6

determine the effect of GFP mutation on GFP expression adn fluorescence