Molecular Basis of Cancer Flashcards
What are the four different cell fates when considering cell homeostasis?
Survival
Growth and divide
Differentiate
Die (apoptotic cell)
What is the process of normal cell proliferation?
- Binding of a growth fractor to its specific receptor
- Transient and limited activation of growth factor receptor
- Activates several signal transducing proteins on the inside of the plasma membrane
- Transmission of signal through cytosol via second messenger or by cascade of signal transduction molecule
- Induces and activates nuclear regulatory factors - initiate DNA transcription
- Entry and progression of the cell into the cell cycle, leads to cell division
What processes need to occur in order for a cell to multiply successfully?
Extracellular signals
For cell-cycle progression - mediated by mitogens binding to cell membrane receptor
For cell growth - mediated by growth factors binding to cell membrane receptors for nutrient uptake and utilization.
What signalling process leads to cell devision from the binding of a mitogen?
Mitogen binds to cell surface receptor (TKR).
Triggers auto and transphosphrylation of intracellular domain
Now active receptor - adaptor protein DRB2 and SOS
SOS is a GEF results in activation of Ras (GTPase)
Activated Ras binds to Raf which autophosphorylates and activates a MAPK signalling cascade - in which MEK and ERK are phosphorylated and activated.
MAP kinase translated into the nucleus - acts as a transcription factor for gene needed for cell division,
Immediate early gene expression of Myc (also a transcription factor) resulting in cyclin D production.
What is the role of NF1 in mitogen activated signalling cascade?
Is a tumour suppressor gene
Negativly regulates the activity of Ras through GTPase activity.
How does growth factor lead to increased growth of the cell?
Binds to cell membrane-bound receptor. (TKR).
Intracellular domains are phosphorylated and activated.
Results in activation of PI-3 kinase
Activation of Akt
1) activates mTOR - results in increased protein synthesis, increased glucose transport through cell membrane transporter, increased glycolysis.
Increased glycolysis means increased pyruvate to enter the mitochondraite for the TCA cycle, results in increase citirete in cytosol
2) activates ATP citrate Lyase - converts citrate to Acetyl CoA - inc lipid synthesis
Results in increased membrane biosynthesis required for cell growth
What is meant by the cell cycle block?
A molecular circuitry in the nucleus, processes and integrates multiple afferent intracellular and extracellular signals to decide if the cell should enter the cell cycle or remain in a non-proliferate state.
Regulates biochemical process responsible for cell division.
Outcomes are - programming cell cycle phases, enter into a quieceint state or enter into active cell cycle.
What is the cell cycle control system?
Regulation of the progression of the cell through the cell cycle - mainly mediated through cyclins and CDK which vary in concentration through the cycle.
Influenced by intracellular and extracellular signalling processes,
Ensures all processes are completed and correct.
Acts as checkpoints
What are the four different classes of cyclins?
G1/S cyclins
S-cyclins
M-cyclins
G1 cyclins
What is the role of G1/S cyclin?
How does its concentration vary throughout the cell cycle?
Activate Cdks in the late G1 and help trigger progression and commitment to the cell cycle
Levels rise in mid G1 and decline rapidly in S phase.
What is the role of S cyclins?
Binds CDKs soon after porgression through Start and help stimulate chromosome duplication.
Elevated from late G1 to mitosis, can control some early mitosis events.
What is the role of M cyclins?
How do their levels vary through the cell cycle?
Activate CDks that stimulate entry into mitosis at G2/M transition.
Increase steadly during G2 and decrease mid mitosis
What is the role of the G1 cyclins and how does their levels vary through the cell cycle?
Govern G1/S cyclins
Help progress through Start in late G1.
Draw a diagram to represent how the level of cyclins and CDKs vary across the cell cycle?
What are the pairs of CDKs to cyclins?
Cyclin D to CDK4/6
Cyclin E to CDK2
Cyclin A to CDK2 or CDC2
Cyclin B to CDC2.
How do cyclin levels fluctuate through the cell cycle?
Mammalian cyclin levels fluctuate dramatically as cell progress through the cell cycle.
Tightly coordinated by cell cycle process as progresses.
heavily influenced by extracellular signals - influence cyclin D which then influcnes other cyclin levels (these other cyclins are considered cell-autonomous)
How are cyclin D activity regulated?
Activity increased by EC ligand binding to receptor, normally mitogens to TKRs.
Highest during G1
At G1/S transition - is exported from the nucleus to the cytoplasm where it can no longer have an effect on cell cycle progression.
What type of signalling can increase cyclin D expression?
RANK receptor - via NFKB
Estrogen receptor - via AP-1 TF
NEr2
Wnt-Frizzled
BcrAbl
And many more
What is the role of Rb in the regulation of activity through the cell cycle?
Rb - TSG
Norm unphosphrylated - binds to and inactivates E2F1
Cyclin D CDK4/6 - phosphorylate - becomes partially active hypophosphorylated - E2F1 - expression of cyclin E.
Cyclin E CDK2 - phosphorylate - becomes hyperphospharylated - inactive TSG - EF2R unbound and able to act as a transcription factor - cyclin A for S cycle.
Cell cycle progression
M to G1 transition - removal of phosphate groups
What is meant by the R point in the cell cycle?
The point of commitment in the cell cycle
Where mitogen signalling is no longer required for progression through the cycle.
Point at which sufficient cyclin E for completion of G1 phase - no longer require increase in Cyclin D.
May also be called to as Start point.
What is the role of E2F1 in the cell cycle?
Is a proto-oncogene
Negativly regualted by Rb, when Rb is hyperophospharylated and inactive
EF21 - acts as transcription factor for genes involved in progression through the cell cycle
Binds to E2F site on DNA, may also recuit epigenetic changes aka histone deactylase and histone methyltransferase to increase gene expression of cyclin A
What cancers does an Rb mutation relate to?
Childhood retinoblastoma - relatively rare - heterozygous congenital mutation.
More common cancers that arise later in life - carcinomas of the lung, breast and bladder - are spontaneous.
What are CDK inhibitor?
Bind to CDK, distort the cyclin binding site and the ATP binding site - reduce catalytic activity of the CDK - limits progression through the cell cycle.
What is the role of p27Kip1?
p27Kip1 is a CDKi
Blocks Cyclin A/ CDK2 function - obstructs ATP binding site in CDK - prevent progression through S phase