Moldule 6 Molecular Biology pt 2

Question 1

Spliceosome, the molecular machinery that processes the eukaryotic pre-mRNA to convert it into the mature form, functions in the

Answer 1

Nucleus

Question 2

Spliceosome is made of

Answer 2

Both RNA and Proteins

Question 3

What part of the pre-mRNA is removed by the spliceosome machinery to make the mature mRNA?

Answer 3

Introns

Question 4

Ribosome, the molecular machinery that processes mature mRNA for the synthesis of proteins, functions in the

Answer 4

Cytoplasm and Endoplasmic Reticulum

Question 5

Ribosome is made of

Answer 5

Both RNA and Proteins

Question 6

The E, P, and A sites on the ribosome are used for protein synthesis by

Answer 6

tRNAs

Question 7

The nucleotide triplets of mature mRNA containing the genetic code are called

Answer 7

Codons

Question 8

The nucleotide triplet of the charged tRNA that matches with mRNA is called

Answer 8

Anticodon

Question 9

Proteins bound for secretion by the eukaryotic cells are synthesized by ribosomes primarily attached to the

Answer 9

Endoplasmic Reticulum

Question 10

Regulation of gene expression in the prokaryotes occurs primarily at the level of

Answer 10

Transcription

Question 11

DNA Methylation is an epigenetic regulation that

Answer 11

Represses Transcription

Question 12

Histone Acetylation is an epigenetic regulation that

Answer 12

Activates Transcription

Question 13

The binding of repressor to the operator of lac operon

Answer 13

Represses Transcription

Question 14

Enhancers are control elements that regulate gene expression. An enhancer region is made of

Answer 14

Nucleotides (DNA)

Question 15

RNA binding proteins regulate gene expression by binding to mature mRNAs at what regions?

Answer 15

5’ UTR and 3’UTR

Question 16

Regulation of gene expression at the level of translation occurs by a ribosomal “Go” or “No Go” mechanism that involves phosphorylation of

Answer 16

eIF2

Question 17

Attachment of Ubiquitin units to proteins flags the proteins for destruction in the

Answer 17

Proteasome

Question 18

Which one of these laboratory techniques allow for amplification of a specific region of DNA within a few (two-to-three) hours?

Answer 18

Polymerase Chain Reaction (PCR)

Question 19

The direction of translation of codons on the mature mRNA by the ribosome occurs in which direction?

Answer 19

5’ to 3’ only

Question 20

During DNA replication, the direction of nucleotide addition by DNA polymerase on the lagging strand occurs in which direction?

Answer 20

5’ to 3’ only