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BATTERY REVIEWER - MOLBIO > MOLBIO - PRELIM > Flashcards

MOLBIO - PRELIM Flashcards

1
Q

What biosafety level is exclusive for primary health services, diagnostic services, research?

A

Basic Biosafety Level 2

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2
Q

What is the ideal depth of the pipet tip to the solution?

A

1 to 3mm

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3
Q

In what circumstances you will manually input the pH in the pH meter?

A

If the temperature differs from the standard room temperature

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4
Q

Which of the following separates the DNA strands in RNA synthesis?

A

Sigma subunit of RNA polymerase

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5
Q

Determine the molarity for 98000 mg of phosphoric acid, H3PO4, in 2.00 L of solution (MW=97.994 g/mol)

A

0.5 M

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6
Q

Development of In – situ hybridization technique is contributed to:

A

Mary – Lou Pardue and Joseph Gal

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7
Q

Which of the following biosafety cabinet provides the worker the highest protection?

A

Class III

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8
Q

Which of the following technique does not need electrophoresis

A

Dot blot

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9
Q

Which of the following solutions is NOT used in cleaning the pH electrode probe?

A

Concentrated NaOH

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10
Q

Which of the following statement is INCORRECT about Absorbance?

A

It is directly proportional to the transmitted light logarithm.

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11
Q

Universal/ Standard Precautions require that

A

Blood and body fluids from all patients be considered infectious

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12
Q

A substance that inhibits the growth and development of microorganisms without necessarily killing them

A

Antiseptic

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13
Q

Which of the following definitions best describes disinfection?

A

The use of antimicrobial agents on inanimate objects to destroy all non-spore- forming organisms

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14
Q

It is used to purify the granulocytes in the Ficoll-Directed Gradient Extraction

A

Ammonium chloride

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15
Q

The following are examples of Personal Protective Equipment EXCEPT:

A

Prescription glasses

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16
Q

Absorbance is also known as?

A

Optical density

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17
Q

Blotting technique for macromolecules that uses electric current is:

A

Western blotting

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18
Q

What is the volume capacity of P20 pipet?

A

2 to 20 ul

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19
Q

Once the liquid is removed from the DNA how can it be stored for years?

A

Keep in freezer

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20
Q

What bond does RNases break to degrade RNA?

A

Phosphodiester bond

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21
Q

The containment principles, technologies and practices that are implemented to prevent the intentional exposure to pathogens and toxin, or their intentional release

A

Biosecurity

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22
Q

Immediate information concerning the health hazards, flammability, and reactivity of a chemical can be obtained from the

A

NFPA symbol

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23
Q

Assurance that the sensitive and valuable information stored in a laboratory is protected from theft

A

Information security

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24
Q

It is a chaotropic agent used in protein degradation

A

Guanidinium thiocyanate

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25
Q

What is the role of protease in DNA extraction?

A

Used to break polypeptides to small fragments

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26
Q

Which of the following is in the correct order regarding DNA extraction?

A

Cell lysis; RNAase treatment; protease treatment &; ethanol precipitation

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27
Q

pH value is temperature dependent.

A

TRUE

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28
Q

The HEPA filters in a BSC filter particulate to size () and are () efficient.

A

0.3µm, 99.97%

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29
Q

To decontaminate Class I and Class II cabinets, this method should be utilized

A

Fumigation with formaldehyde gas

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30
Q

Sample problem: If O.D. at 260 is 0.30 nm
O.D at 280 is 0.45 nm Dilution factor is 100. Compute for dsDNA concentration.

A

1500 ug per ml

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31
Q

Example of RNase inhibitors

A

DEPC

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32
Q

Termination of transcription is facilitated by

A

Rho factor

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33
Q

It is used to digest the cell walls of fungal cells

A

Zymolyase

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34
Q

Double door entry is found in

A

BSL-3 and BSL-4

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35
Q

Which of the following is NOT a step in DNA replication?

A

Splicing

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36
Q

Adenine forms a covalent bond with a nucleic acid sugar at

A

N9

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37
Q

This is considered as the backbone of Biosafety

A

Risk assessment

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38
Q

Which substance was used to break down the phospholipids from the cell membrane and nuclear membrane?

A

Soap

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39
Q

What substance can cause the DNA to precipitate?

A

Ethanol

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40
Q

During electrophoresis, denaturation of the double stranded DNA molecule is brought by:

A

Treatment with alkali

41
Q

How many grams would you prepare in 100ml of a 7.5% (w/v) solution of NaCl?

A

7.5g

42
Q

If the ratio between OD 260 and OD 280 is
0.63 what does that tell us?

A

The sample is contaminated with other proteins

43
Q

What is the purpose of adding Proteinase K in FFPE?

A

To increase the DNA yield

44
Q

Which of the following RNase inhibitors needs to be removed from final preparation?

A

DEPC

45
Q

This equipment is used to accurately measure and dispense small volumes of liquid

A

Pipettors

46
Q

What is the main difference between RNA and DNA?

A

RNA has ribose DNA has deoxyribose

47
Q

When does splicing occur?

A

Following pre-mRNA transcription in the nucleus

48
Q

Which of the following is the basis for Western blot?

A

Antigen – Antibody reaction

49
Q

Which of the following practices should be utilized when working in a biological safety cabinet?

A

Allow the BSC to operate before work begins and after work ceases

50
Q

It is the most effective and reliable means to sterilize laboratory materials and decontaminate waste materials

A

Steam under pressure

51
Q

What is the importance of magnetic beads in RNA extraction?

A

RNA binding

52
Q

What is the next step after the addition of Elution buffer?

A

Add TE buffer

53
Q

Why Bunsen burner must not be used in the biosafety cabinet?

A

The heat produced will distort the airflow and may damage filters

54
Q

Sample problem: If O.D. at 260 is 0.30 nm
O.D at 280 is 0.45 nm Dilution factor is 100. Check for the contamination.

A

0.67 nm

55
Q

Which of the following biosafety cabinet does not have HEPA filtered supply air?

A

Class I

56
Q

RNA molecules that carry amino acids to the ribosome

A

tRNA

57
Q

Dilute 150mL of 10X TBE buffer stock to 1.5L with deionized water.

A

1X

58
Q

What level of biosafety practice Good Microbiological Techniques plus protective clothing only?

A

BSL 1

59
Q

The following terminate protein synthesis EXCEPT

A

UGG

60
Q

Which of the following is FALSE for RNases?

A

Preserve RNA in the sample

61
Q

Southern blotting is a techniques used to:

A

Identify specific DNA

62
Q

Equipment used in Molecular Biology to visualize DNAs and proteins

A

UV transilluminator

63
Q

What is the tool that we used to measure pH in our laboratory activity?

A

pH meter

64
Q

In reagent preparation activity, what instrument is used to measure the weight of the chemicals?

A

Analytical balance

65
Q

Which of the following statement/s is/are true regarding Southern blotting?

A

-Developed by Dr. Edwin Southern
-DNA – DNA hybridization is the basis
-Transfer of DNA fragments from gel to nitrocellulose membrane is done

66
Q

TRUE for handling and storage of Biological waste

A

Must be placed in a bag marked with biohazard symbol

67
Q

How many ml of a 2.50M NaOH solution are required to make 525ml of a 1.50M NaOH solution?

A

315ml

68
Q

Which of the following is NOT a function of nucleic acid?

A

They distribute protein all throughout the body

69
Q

Filters generally used in biological safety cabinet to protect the laboratory worker from particulates and aerosols generated by microbiology manipulations are

A

HEPA

70
Q

Autoclave temperature

A

121°C

71
Q

ALL pipets have volume adjustment knob.

A

FALSE

72
Q

Calculate the molarity of 500ml of 1.9 x 10^-6moles of NaCN in a

A

0.0000038 M

73
Q

What does DNA stand for?

A

Deoxyribonucleic acid

74
Q

A microorganism that is unlikely to cause human or animal disease

A

Risk Group 1

75
Q

Labelled antibodies are used to detect:

A

Presence of a particular Protein molecule in Western Blot

76
Q

Which of the following statements is FALSE?

A

Pipetting of BSL-3 materials should be done in an open bench top

77
Q

How many grams of sodium chloride are needed to prepare 1L of 0.9% normal saline?

A

9

78
Q

DNAs are found in a prokaryotic cell.

A

TRUE

79
Q

It is a multifaceted cellular response in which more than 40 genes are induces when DNA is damaged

A

SOS repair

80
Q

The first step in this technique is to depress the plunger down to 1st stop.

A

Forward pipetting

81
Q

Which of the following is neutral pH?

A

7.00

82
Q

Where does protein synthesis takes place?

A

Ribosome

83
Q

In the final step of purification, DNA is rehydrated with what type of buffer?

A

Tris-EDTA

84
Q

A technologist spilled 10 gallons of formaldehyde on the floor. After determining the chemical poses a significant health hazard, the first action step would be to:

A

Evacuate the area

85
Q

The first step in this technique is to depress the plunger down to 2nd stop.

A

Reverse pipetting

86
Q

Which of the following appears as a film of debris at the interphase after centrifugation of Organic Extraction Method?

A

DNA

87
Q

Which of the following is not true for TRIzol?

A

used for purification step

88
Q

The principle of capillary transfer is applied in which technique?

A

Northern Blot

89
Q

Which of the following is INCORRECT when it comes to PH meter handling?

A

2 point calibration is better that 3 point calibration

90
Q

After centrifugation, it is the solid form found at the bottom of the container

A

Precipitate

91
Q

For a safe operation of a centrifuge:

A

Ensure proper balance is maintained

92
Q

This type of extraction relies on the adsorption of nucleic acids to the solid phase

A

Minicolumn purification

93
Q

In Western blotting:

A

Polyacrylamide gel is commonly used

94
Q

When the hand is visibly soiled, it is recommended to decontaminate the hands with

A

Soap and water

95
Q

Which of the following steps is arranged accordingly?

A

Mix solution to be measured; Remove electrode from storage buffer solution; Wash the electrode with distilled water

96
Q

A solution is 0.2500M H2SO4. Calculate the volume in ml present in a sample of the solution that contains 0.005216moles of sulfuric acid

A

20.864ml

97
Q

What is the volume capacity of P1000 pipet?

A

200 to 1000 ul

98
Q

Which of the following does not describe Guanidine thiocyanate-phenol-chloroform extraction?

A

DNA molecules and proteins remain in the upper layer of the tube

BATTERY REVIEWER - MOLBIO (3 decks)

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