Module 6.1.3 - Manipulating Genomes Flashcards
1
Q
What is a genome?
A
Minimum quantity of genetic material that contains 1 copy of all genes of an individual or of a population or a species
2
Q
What is genomics?
A
Study of whole set of genetic info in form of DNA base sequence in cells of organism
3
Q
What is gene technology?
A
Manipulation of organism’s DNA to produce an organism or product that can be used in some way
4
Q
What are autosome?
A
Chromosomes that arent sex related
5
Q
What are terminator bases?
A
Can be any base, modified bases that stop the chain
6
Q
What is the sanger method?
A
- based on premature ending of DNA synthesis
- if modified nucleotides are used during DNA synthesis, process can be halted
- DNA being sequenced
- mix of normal nucleotides and 1 type of terminator nucleotide
- primer starts it off
- DNA polymerase
7
Q
What is gel electrophoresis?
A
- separates different lengths of DNA
- phosphate in backbone of DNA is negatively charged
- DNA placed at top
- electric current applied over it
- ager resists movement of DNA fragments
- DNA moves towards positive electrode
- DNA moves at different rates
- longer sequences dont move as far/near top
- shorter sequences move further towards bottom
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