Module 6 Flashcards
Griffith experiments
dead type S cells were able to transform type R cells into type S through DNA
Avery, MacLeod and McCarty
Only DNase was able to stop transformation, not RNase or protease. So the genetic information substance must be DNA, not RNA or proteins
Hershey and Chase
DNA gets injected into cells from viruses, not proteins –> DNA must be genetic material
A nucleotide has
a phosphate group, a pentose sugar, and a nitrogenous base
Purines (double ring)
A and G
Pyrimidines (single ring)
T, C, and U
DNA directionality
5’ to 3’
phosphodiester linkage
connects the 5’ carbon of one nucleotide to the 3’ carbon of an adjacent nucleotide
The backbone is comprised of
phosphate groups and sugar
Linus Pauling
proposed a-helix (single strand)
Rosalind Franklin
found that DNA must be helical and have more than one strand
Chargaff’s rule
percent A = percent T and percent C = percent G
Watson and Crick
double strand helix
DNA sequences are necessary for
synthesis of RNA and cellular proteins, Replication of chromosomes, proper segregation of chromosomes, and compaction of chromosomes
prokaryotes
bacteria and archaea, circular chromosomal DNA, single type of chromosome
DNA supercoiling
the formation of additional coils due to twisting forces, a way to compact the DNA
Positive supercoil
twisted in the same direction as the DNA is wound, no stress on strands
negative supercoil
twisted in the opposite direction as the DNA is wound, stress on strands help them separate for replication and transcription
introns
noncoding intervening sequences
Origins of replication
chromosomal sites necessary to initiate DNA replication, occur about every 100,000 bp
Centromeres
Regions that play a role in segregation of chromosomes
telomeres
prevent chromosome shortening
unique or non-repetitve sequences
found once or twice in a genome, includes protein-coding genes and intergenic regions, make up ~41% of human genome
moderately repetitive sequences
found a few hundred to several thousand times, genes for rRNA and histones, sequences that regulate gene expression and translation, transposable elements
highly repetitive sequences
found tens of thousands to millions of times, each copy is relatively short, some sequences are interspersed throughout the genome, other sequences are clustered together in tandem arrays
tandem arrays
non-transposable elements
Transposition
the integration of small segments of DNA into a new location in the genome
Transposable Elements
small, mobile segments of DNA
Simple transposition
TE moves to a new target site
Retrotansposition
TE moves via an RNA intermediat
Direct Repeats
flank TEs, identical base sequences that are oriented in the same direction and repeated
Inverted Repeats
DNA sequences that are identical but run in opposite directions, contains gene for transposase, which catalyzes the transposition event
LTR retrotansposons
contain long terminal repeats at both ends
autonomous elements
TEs that contain all of the information necessary for transposition or retrotransposition
non-autonomous elements
TEs that lack a gene that is necessary for transposition
transposase removal
recognizes the inverted repeats at the ends of a TE and brings them close together to dimerize, cleave the outside of the IRs and remove the segment
transposase insertion
cleaves the target DNA at staggered sites (one on each strand), inserts the TE, DNA repairs sections with gaps creating direct repeats on both ends of the TE
Reverse transcriptase
TE –> RNA –> synthesizes a new double stranded DNA molecule –> intergrase adds new strands to old DNA strand by recognizing LTRs
LINEs
Long Interspersed elements
SINEs
Short interspersed elements
Nucleosome
8 histone proteins + 146 or 147 bp
SMC proteins
cause loops to form
CCCTC binding factor
stabilize loops
Heterochromatin
tightly compacted regions of chromosomes, transcriptionally inactive
Euchromatin
less condensed, transcriptionally active
Condensin 1
creates big main loops during chromosome condensation
condensin 2
creates smaller loops within the big main loop
cohesin
promotes binding between sister chromatids during mitosis and meiosis