Module 5 - Protein Flashcards
What is Protein
- Component of every living cell
- all proteins are made up of amino acids
- every AA contains Nitrogen
Peptide bonds
Covalent bond that links AA together to form a protein
Dipeptide
2 amino acids coming together
Water is released
Tripeptide
Bonds have 2 peptide bonds
Primary structure
Linear order of AA
Secondary Structure
Local spatial arrangement of polypeptide backbone.
Tertiary Structure
Final 3D shape of protein.
What happens to protein when it undergoes ‘denaturation’
Protein denature means (destroying 3D shape) but doesnt break any peptide bonds.
example/ protein hitting stomach acid.
Red Blood Cell Vs Sickle Cell anemia
PolyPeptide
Normal: Normal
Sickle: Altered
Hemoglobin Shape
Normal: Normal
Sickle: Altered
Hemoglobin Arrangement
Normal: Individual Molecules
Sickle: Long chains of Molecules
Red Blood Cell shape
Normal: Disc
Sickle: Sickle
Sickle Cell
Happens when there is reduced oxygen carrying capacity
Proteins that regulate “body processes”
Some Hormones
- Insulin, Glucagon
Enzymes
- Lipase, Amylase, Trypsin
Neurotransmitters
- Serotonin
Proteins that help Transportation substances throughout
Hemoglobin - Transports oxygen
Transferrin - Blood transport proteins for iron.
Proteins that work as apart of immune defence
Antibodies - proteins with sugar groups attached to them
Proteins that growth and repair tissues
Muscle Protein:
a) Actin/Myosin
b) Collagen
Normal turnover (breakdown + Replacement)
How does Stomach help in protein digestion
- HCL Denatures proteins
- Protein begins digestion in stomach –> PEPSIN enzyme lives in stomach to break down peptide bonds.
how does Pancreas help in protein digestion
Produces 2 enzymes Trypsinogen, Chytrypsinogen
Pre enzymes
How does Small Intestine help in protein digestion
- Peptides enter
- Intestinal cells produce Enterokinase (Active enzyme)
- Pancreas produces Trypsinogen, Chytrypsinogen
- Enterokinase turns trypsinogen to trypsin which turns it into active enzyme
- Chymotrypsinogen gets turned into chymotrypsin by trypsin enzyme
- Then broken down into individual AA
- Amino Acids go to portal system (blood)
Amino acid Metabolism
AA in blood system which heads to liver first
1. Synthesis of AA –> Required body proteins
2. Convert AA –> Glucose
3. AA used directly by liver for energy (4kcal/g)
4. Converted to fat.
What does HCL do in protein digestion
Denatures Proteins
What does Pepsin do in protein digestion
Enzyme that lives in stomach and breaks down peptide bonds.
What does Trypsin do in protein digestion
Turns Chymotrypsinogen to chymotrypsin
What does Chymotrypsin do in protein digestion
Breaks down the proteins into indivdual amino acids
Various ways AA enter liver from portal vein.
Bloodstream
Diet (exogenous)
Tissue Breakdown (endogenous)
Explain the difference between complete and incomplete dietary proteins sources
Complete proteins contain all nine essential AA
Incomplete Proteins contain some but not all 9 AA
Example of Complete proteins
Meat
Poultry
Eggs
Fish
Diary
Examples of incomplete protein foods
Nuts
Legumes
Grains
Veggies
9 Essential Amino Acids
PVT TIM HLL
Phenylalanine
Valine
Threonine
Tryptophan
Isoleucine
Methionine
Histidine
Leucine
Lysine
Limiting amino acids
Amino acids in shortest supply relative to body requirements for absorbed AA
Health Benefits of a plant-centred diet.
Lower body weight
reduce incidence of obesity and other chronic diseases such as diabetes, cancer and cardiovascular disease.
Design a Vegan Meal
Rice & Beans
Rice & Lentils
Bread & PB
Tofu and Cashew
Bean burrito
Poorly-Planned vegan diet concerns
Protein Deficiencies
Vitamin B12 deficiency
3 examples of a person in a negative balance
Losing body weight
Disease
Inactivity
3 examples of a person in a positive balance
Gaining weight
Repair (broken leg)
Athlete activity
How do figure out How much protein needed DRI values
lbs/ 2.2 = weight in kg
weight in kg x 0.8 = amount of protein a day
Protein requirement needed for Non-Athlete, Endurance Athlete, Power Athlete
Non-Athelete is 0.8
Endurance is 1.2-1.4
Power is 1.2-1.7
Potential Risks of taking single amino acid supplements
GI Tract - If we take in to much AA we start losing Trp, Tyr, Phe essential AA
Brain - Trp turns into serotonin
Becomes AA imbalance
Is too much protein bad?
- Increase calorie intake
- High protein foods often high in fat
- Calcium loss in urine increases
- Damages Kidneys If you are type 2 diabetes