Module 5: Heredity Flashcards

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1
Q

Asexual reproduction

A

Requires only one parent-A parent cell replicates and divides generating offspring that are genetically identical.
-Contains the same genetic material as the parent
EG: Bacteria- binary fission

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2
Q

Sexual reproduction

A

Requires two parents.
-A haploid (n) gamete from each parent combines to produce genetically unique diploid (2n) offspring
-EG: Humans- egg and sperm will meet

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3
Q

Haploid and diploid

A

Haploid: one copy (n), diploid: two copies (2n)

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4
Q

How do plants sexually reproduce?

A

-Flowers are key in plant sexual reproduction (they are the reproductive organs of plants)
-The fusion of two haploid gametes
-They produce male sex cells (pollen grains) and female sex cells (ovum- contained in the ovules)
-These two must meet for pollination and for reproduction to begin.
-After pollination occurs, fertilization occurs and the ovules grow into seeds in a fruit which disperse once ripe.
-Stamen (male), pistil (female)

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5
Q

How do plants asexually reproduce?

A

Bulbs:Underground storage organ that consists of a short stem surrounded by fleshy leaves. New shoots (plants) develop from the bulb. (Example- Onion)
Runners: Side branches that grow close to the ground and develop new plantlets on them.(Example- Strawberries)
Apomixis: Some plants are able to produce offspring from special generative tissues, without involving fertilisation or the production of seeds.This generative tissue gives rise to new plantlets that produce asexual seeds. Plantlets that arise on leaves and their seeds grow into individuals that are
genetically identical to their parents.(Examples-Kangaroo Grass)

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6
Q

How are gametes produced in animals?

A

-Gametes are produced by each parent organism through meiosis
-Each gamete contains half the necessary number of chromosomes- through ext or int fertilisation

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7
Q

Internal fertilisation (advantages and disadvantages)

A

Increased likelihood of fertilization as egg and sperm are in close proximity- increased protection from the environment- leading to higher survival rates of offspring.

Fewer offspring are produced, more difficult to bring makes and females into contact- higher risk of sexually transmitted infections etc

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8
Q

Internal fertilisation

A

Sperm and egg come together within the female body (in mammals it is the result of sexual intercourse)

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9
Q

External fertilisation

A

Sperm and egg unite outside of the female body (amphibians, fish-usually an aquatic environment)

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10
Q

External fertilisation (disadvantages and advantages)

A

Results in the production of a large number of offspring, easier to find mates as the gametes released can drift (wind, water etc), ore genetic variation, less competition- due to spread
Simpler behavioural process

Environmental hazards such as predators reduce the chance of surviving into adulthood. Gametes can go unfertilized and wasted.
Not guaranteed that sperm will encounter eggs. Greater chance of desiccation of gametes/zygotes

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11
Q

Asexual reproduction in animals

A

Budding:Daughter cell develops in the mode of an outgrowth or a bud (pinching off) from the parent cell by mitosis. (Coral)
Fragmentation: Where the body of an organism breaks into two or more types, each of which develops into a new organism due to the presence of stem cells.
Parthenogenesis: Where offspring develop from the gamete without prior fertilization from the male gamete (unlike sexual reproduction

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12
Q

Reproduction in bacteria (asexual binary fission

A
  1. The cell elongates- building more cell walls (full adult size)
  2. The bacterial genome replicates and remains attached to the membrane. At the same time any plasmids (small circular DNA) that are present, replicate.
  3. Duplicated DNA begins to separate, moving towards the poles as the cell elongates more.
    4-5. Cleavage furrow begins to form in cell walls, in cleavage furrow.
  4. Two identical daughter cells are produced.
    -Some bacterial species use other patterns of cell division to reproduce- some grow larger than the adult size, undergo multiple divisions and produce many offspring.
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13
Q

Reproduction in fungi

A

Sexual: Specialized haploid (n) hyphae fuse together to form a diploid cell (2n). This genetically unique diploid cell then undergoes meiosis to produce new haploid (n) spores
Asexual: Fragmentation, budding, spores (Pieces of hyphae can separate and become their own colonies, mitosis produces genetically identical cells to the parent, which are distributed into the environment by wind or vectors etc)

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14
Q

Reproduction in protists

A

Sexual: Two haploid cells fuse to form a new cell, undergoes meiosis to form new haploid cells, or diploid cell undergoes meiosis to produce 4 gametes that fuse during fertilisation

Asexual: Binary fission (replicates genetic material and then splits), budding (gives rise to small bud that separates), multiple fission (several individuals form out of the parent)

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15
Q

Advantages and disadvantages of sexual reproduction

A

High genetic diversity
-Less prone to environmental change
-Facilitates adaptation

-Energy costly
-Requires two parents
-Courtship is time and resource-consuming

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16
Q

Advantages and disadvantages of asexual reproduction

A

-Energy efficient
-Requires 1 parent only
-No courtship is required
-High amount of gametes

Low genetic diversity
-More prone to environmental change
-Inhibits adaptation and evolution

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17
Q

Why do some organisms reproduce both ways?

A

-Organisms that can produce both sexually and asexually, are able to receive the advantages from both.
-When the number of males is high and the conditions aren’t favourable in a certain area, organisms are more likely to sexually reproduce than asexually reproduce.

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18
Q

Process of fertilisation itself (sperm and egg fusion)

A

1.Sperm makes contact with the egg and burrows through corona radiata.

2.Sperm attaches to receptor of zona pellucida

3.Digestive enzymes realeased across the acrosome and sperm burrows through zona pellucida.

4.Plasma membranes of sperm and egg fuse.

5.Sperm nucleus enter egg.

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19
Q

Process of implantation

A
  1. Fertilisation
    2.Zygote
    3.Morula 3-4
  2. Blastocyst 4-7
  3. Implantation 74
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20
Q

LH

A

-Helps control the menstrual cycle, triggering the release of an egg from the ovary- helps the development of the egg
-Secreted by the pituitary gland

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21
Q

FSH

A

Helps control the menstrual cycle and stimulates the growth of eggs in the ovaries.

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22
Q

Progesterone

A

-Helps prepare the uterus for the implantation of a fertilized egg and maintains pregnancy.

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23
Q

Estrogen

A

Regulation of the reproductive system.

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24
Q

GnRH

A

-Triggers the ovaries to make progesterone and estrogen
-Triggers the pituitary gland to secrete LH and FSH
-Produces estrogen and progesterone to regulate ovarian and menstrual cycles.
-Secreted by the hypothalamus

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25
Q

What do oestrogen and progesterone do?

A

Estrogen and progesterone levels regulate ovarian and menstrual cycles and create ideal conditions for ovulation, implantation and maintaining pregnancy.

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26
Q

What is artificial pollination?

A

1.Pollem (sperm) removed from the stamen of one plant
2.Pollen applied to the stigma of another plant
3. Pollen fertilizes the ovum.
-Both agricultural and domestic plant breeders use this process to breed plants with specific characteristics such as colour, size and yield.
(VANILLA- one species of bee)

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27
Q

What is artifical insemination?

A

1.Detection of female cows in oestrus
2.Collection of semen
3. Insemination usually performed using an insemination gun which shoots semen into the cervix of the desired animals.

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28
Q

Selective breeding?

A

-The intentional mating of individuals with desirable traits in the hope that offspring will express those same traits in their phenotype
-Its prevalent in sheep pigs, cows, chickens, fish, prawns etc
-Sheep have been selected for the quality and quantity of the wool they grow and beef cattle for their muscle mass
1.Determine the desired breed
2.Interbreed parents that show the desired trait
3.Select the offspring with the best form of the trait and breed these offspring
4.Continue this process until the population reliably produces the desired trait
-EXAMPLE: Jersey or Angus cows

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29
Q

Genetic engineering

A

-Process of a gene being removed from one species and inserted into the genome of another species
-The protein produced by the gene is then expressed in tge genotype of the second species

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30
Q

Genetic engineering plants and animals examples

A

BT
-Bt is a bacteria species producing a toxic that is toxic to select insect pests
-Gene is isolated from BT and inserted into cotton and rice to produce a transgenic species called BT cotton or rice.
-Natural insecticide that kills insects- higher yield

GM Atlantic salmon
-Growth hormone from the chinook salmon and a gene from the eel-like ocean pout which switches on the Chinook’s growth hormone
-Allows them to grow all year round.

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31
Q

Cloning

A

Cloning is the process by which genetically identical copies of an organism are made without using the process of sexual reproduction.
The most common method (and the oldest) is grafting. The plant and fruit produced is a clone of the original plant. This is seen commonly in fruit crops.

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32
Q

IDEA- Contributions of scientists

A

Without knowing Mendel, Chargaff and Watson and Crick we wouldn’t be able to manipulate at all.

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33
Q

Mitosis

A

-Cell division resulting in two identical daughter cells, with the same number and kind of chromosomes as the parent cell.
-Mitosis plays an important role in growth, repair, asexual reproduction and genetic stability

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34
Q

Interphase of Mitosis

A

Cell prepares itself for division
-DNA replication occurs to produce two copies of each chromosome.
-Period of growth and normal activity

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35
Q

Prophase

A

Duplicated chromosomes condense
-Chromosomes become visible and the centrioles separate and move to opposite poles of the cell.
-Microtubules that are apart of mitotic spindles that lengthen and shorten to pull chromatids apart.

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36
Q

Metaphase

A

Prometaphase: Nuclear envelope breaks down
-Pairs of condensed chromosomes (called sister chromatids) line up along the equator of the cell
-Each pair of chromosomes are attached to different spindle fibers to move in the opposite direction.

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37
Q

Anaphase

A

Chromatids separate and move towards opposite poles of the cell by the mitotic spindle
There is now only one copy of each chromosome at either end of the cell.

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38
Q

Telophase

A

Two new nuclear envelopes form around the separated sister chromatids
Chromosomes spread out again
-Nucleolus reappears and spindle fibers disappear

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39
Q

Cytokinesis

A

Two daughter cells are separated by. The tightening of a ring of proteins around the middle of the dividing parent cell- the two nuclei are squeezed apart
-Cytokinesis occurs simultaneously to anaphase and telophase- the pinching of the cellular membrane begins to happen as chromosomes are separated and new nuclei are formed.

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40
Q

Meiosis

A

-Cell division resulting in four genetically unique daughter cells (gametes) each with half the number of chromosomes (haploid) of the parent cell (diploid)
-Creates sex cells- half as many chromosomes as mitosis- iInvolves two successive divisions of the nucleus

41
Q

Homologous chromosomes

A

Chromosomes that share the same structural features, carrying the same kind of genetic information, but may have different alleles.

42
Q

Independent assortment

A

Process where chromosomes move randomly to separate poles during meiosis (Metaphase 1)

43
Q

Meiosis 1: Interphase

A

-As with mitosis, DNA replication occurs to produce two copies of each chromosome.

44
Q

Meiosis 1: Prophase

A

Chromosomes condense and the nuclear envelope breaks down (prometaphase)
-Homologous chromosomes pair up, aligning next to each other along their full length
-Crossing over occurs between homologus chromosomes- segments of DNA at the same locus to create new gene combinations.

45
Q

Meiosis 1: Metaphase

A
  • Homologous pairs (not individual chromosomes) line up along the equator of the separating cell.
46
Q

Meiosis 1: Anaphase

A
  • Homologous pairs are separate, pulled to opposite ends of the cell by the mitotic spindle
    -Sister chromatids remain attached
47
Q

Meiosis 1: Telophase

A

-Chromosomes arrive at opposite ends of the cell
-Two diploid daughter cells are formed by cytokinesis.

48
Q

Meiosis II: Prophase II

A

-Chromosomes condense and the nuclear envelope breaks down

49
Q

Meiosis II: Metaphase

A
  • Chromosomes line up along the equator of the dividing cells.
50
Q

Meiosis II: Anaphase

A
  • Sister chromatids are separated by the spindle microtubules and pulled towards opposite poles of the cell.
51
Q

Meiosis II: Telophase

A

-Cytokinesis splits the dividing cell into two new cells
-Nuclear membranes form around each set of chromosomes
-Four haploid daughter cells are formed each containing half the number of chromosomes of the parent cell.

52
Q

Meiosis II: Cytokinesis

A

Four fully seperate cells

53
Q

Nucleotide

A

A monomer of DNA that consists of phosphate group joined to a deoxyribose sugar which is attached to a nitrogenous base of adenine, thymine, cytosine or guanine.

54
Q

Base

A

Also called a nitrogenous base. Bases are the nitrogen-containing molecular units that make up a nucleic acid.

55
Q

Double helix

A

A pair of parallel spirals intertwined around a common axis.

56
Q

The general structure of DNA

A

There are two-sugar phosphate backbones on the outside, which are held together by hydrogen bonds between pairs of nitrogenous bases on the inside.

57
Q

Hydrogen bond

A

An attractive force between the hydrogen attached to an electronegative atom of one molecule and an electronegative atom of a different molecule.

58
Q

DNA replication (UUHBPJ)

A

DNA replication is the process by which the double stranded DNA molecule is ‘unzipped’ by enzymes and free nucleotides bind t each of the exposed backbones to create two identical DNA molecules. Cells copy genetic information before cell division so that each new cell has a complete set of DNA.

59
Q

DNA replication steps

A

Unwind (Topoisomerase unwinds the coiled strands of DNA), unzip (DNA helicase unzips the strands of DNA< breaking the hydrogen bonds creating two template strands for replication, HO (Single strand binding proteins (SSB) keep the strands seperated, BP (DNA polymerase III bonds free nucleotides with nucleotides on each parents strand using base pairing rules, proofread (DNA poly1 proofreads new strands and backtracks over errors), JN (DNA ligase bonds backbone back together)

60
Q

The direction of DNA replication

A

-Complimentary base pairing results in double-stranded DNA
-Both DNA strands run anti-parallel to each other (oriented in different directions)
-One strand is 5’-3’ while the other is 3’-5’.
-DNA ONLY REPLICATES IN THE 5’ TO 3’ DIRECTION: replication will be continuous for one strand (the leading strand) and discontinuous for the other strand (the lagging strand)

61
Q

Leading and lagging strands, replication fork

A

: Directional movement of replication
-Nucleotides area added in the SAME direction as the replication fork- creating a continuous leading strand.
-Nucleotides added in the opposite direction as the replication fork creates okazaki fragments on the lagging strand.
-Polymerase III only works in 5’ to 3’ direction on BOTH parent strands- THUS creating continuous replication on the leading strand.

62
Q

Okazaki fragments

A

Short sequences of DNA nucleotides that are synthesized discontinuously and later linked together by DNA ligase to create the lagging strand in DNA replication.

63
Q

How does DNA repair foster the continuity of species?

A

-The insertion of an incorrect base is common during DNA replication
-A repair enzyme detects these and snips it out, replacing it with the correct base
-This is called a DNA mismatch repair- function of DNA polymerase I.

64
Q

How do errors in cell replication affect the continuity of species?

A

-If errors aren’t picked up quickly and remain undetected they can replicate and cause a permanent mutation.
-If the mutation occurs during meiosis- it can be passed on to the offspring as well (importance of replication accuracy)

65
Q

How does mitosis affect the continuity of species?

A

-Increases the number of cells in an organism, allowing for development of a multicellular body
-Mitosis allows for cell renewal and replacement- ensuring an effective function
-Mitosis allows human to develop to maturity when we can pass our genetic information onto offspring through sexual reproduction
-Some organisms reproduce asexually- facilitated by mitosis
-In this case, mitosis creates the next generation of organisms (species continuity)

66
Q

How does meiosis affect the continuity of species?

A

Combination of gametes during sexual reproduction creates new organisms- inherited traits from both parents
-Meiosis purposefully introduced variation
-Processes of crossing over, independent assortment, and random segregation allow for combos of different alles- increasing genetic variation in offspring and wider species population.
-The genetic diversity caused by meiosis is important for the continuity of species as mutation and variation are essential factors for survival and evolution.

67
Q

How does DNA replication affect the continuity of species?

A

The replication of genetic material ensures that information important for life is transferred down through generations
-If DNA wasn’t replicated before mitosis and meiosis (during interphase), cell division would have half the amount of DNA, and resulting cells would die- inadequate amounts of genetic information
-DNA replication is a high fidelity process: ensuring that daughter DNA strands carry the same gene- encoding all the essential proteins for life

68
Q

Prokaryotic DNA

A

The prokaryote has a circular, double-stranded piece of DNA, not surrounded by a nuclear membrane
-Small circular, independent, double-stranded DNA molecules
-DNA is not bound by proteins (such as histones like in eukaryotic DNA) -Proteins have smaller, more compact genomes, little repetitive DNA
-Has small and extra chromosomal segments of DNA called plasmids

69
Q

Eukaryotic DNA

A

Linear, associated with histones and is found in the nucleus
-Chromosomes are paired

70
Q

Swithcing on genes

A

A gene that is switched ON is transcribed, translated, turned into a polypeptide and thus a functional protein (special proteins called transcription factors or DNA binding factors control which genes are transcribed by binding to a specific DNA sequence)

71
Q

Effect of soil pH on hydrangea colour

A

-Flower color is determined by soil ph.
-The pH effects the availability of other ions in the soil and these ions are responsible for a color change.
-pH less than 5= blue flowers in acidic soil
-pH more than 7= neutral and alkaline soul=pink flowers.

72
Q

What are polypeptides and proteins?

A

Linear molecules, made up of multiple peptides

Are the functional unit, made up of one or more polypeptides.
-Proteins have a very defined structure determined by the sequence and folding of amino acid chains. This ultimately determines the function of the protein.

73
Q

What is transcription?

A

The process by which a complementary copy (mRNA) of a gene (DNA) is made in the nucleus.

74
Q

What is translation?

A

The process of turning information encoded as mRNA into a polypeptide chain (to then become proteins)
-It uses mRNA codons (3 sets of bases) as the template for coding the sequence of amino acids to form a polypeptide chain
-RNA anticodons carry the appropriate amino acids into place
-The genetic sequence of the mRNA is read by ribosomes
-The code is translated in groups of three nucleotides called a codon

75
Q

Steps of transcription

A

-RNA polymerase binds to the promoter sequence upstream from a gene
-As RNA polymerase moves along the DNA strand, a mall region of DNA is unwound
-RNA polymerase ‘reads’ the DNA template strand, matching complementary free-floating nucleotides to create an mRNA chain containing the same coding information.
-A mRNA molecule is sequentially synthesized by RNA polymerase as it continues to move along the DNA strand
Terminator sequences end the transcription of DNA, and the newly formed RNA molecule is released.

76
Q

Steps of translation

A

-mRNA docks to a ribosome
-The ribosome matches a complementary tRNA molecule to the mRNA by matching codon-anti-codon sequences
-As subsequent tRNA molecules dock, a polypeptide forms between the adjacent amino acid molecules they carry
-As the mRNA molecule continues to be read, the polypeptide chain is elongated by continued addition of amino acids
-When a stop sequence is reached, the ribosome releases mRNA and polypeptide molecule
-The polypeptide folds and undergoes post-translational modifications, resulting in a mature protein, ready for use within the cell.

77
Q

Significance of messenger RNA

A

-DNA doesn’t leave the nucleus, as a result, a message must be sent from the nucleus to the ribosomes where protein synthesis occurs.
-mRNA is essential to carry the genetic code stored in DNA from the nucleus to the ribosomes in cytoplasm

78
Q

Significance of transport RNA

A

tRNA carries the specific amino acid to be joined into the polypeptide chain
-As a result of the contemporary nature of the mRNA and tRNA codon-anti-codon complex, the specific sequence of amino acids required for protein synthesis occurs.

79
Q

Incomplete dominance

A

-Incomplete dominance occurs when one allele for a specific trait is not completely expressed over its paired allele
-This results in a third phenotype in which there is a blending of the alleles in their own phenotype

80
Q

Codominance

A

Co-dominance occurs when alleles of a gene pair in a heterozygote are both fully expressed in the phenotype-neither are dominant or recessive

81
Q

X linked dominant

A

Affected daughters from affected fathers

82
Q

X linked recessive

A

Affected sons from affected mothers

83
Q

Population genetics

A

-Population genetics is the study how the gene pool of a population changes over time, leading to a species evolving.

84
Q

Allele frequency

A

Freq of G= number of copies of allele G in population

divided by

total number of copies of the gene G+g in the population

85
Q

SNP

A

Single nucleotide polymorphism (SNP- pronounced SNIPS) is a point mutation (single base G, C or A T) in a segment of DNA that occurs in more than 1% of the population

86
Q

Why are SNPs useful

A

-SNPS are important genetic markers that are currently used to distinguish individuals and to identify things such as disese succeptibility in individuals.
-A genetic marker can be defined as an identified sequence of DNA at a locus on a chromosome

87
Q

GWAS

A

-A genome-wide association study (GWAS) rapidly scans SNP markers across the genomes of individuals with a known disease or disorder to compare them to control individuals
-Significant differences in allele frequency caused by SNPs are the first step in identifying cause and effect relationship between a SNP and a disease or disorder
-Once a link is made, scientists look to develop better treatments and diagnostic and prevention strategies.
-GWAS have identified DNPs related to conditions including cancer, diabetes, heart disease, mental illness, Parkinsons, Crohns and Alzheimer’s

88
Q

Hardy-Weinberg equation

A

For a diploid gene with two alleles (B and b) at frequencies p and q, the frequency of genotypes BB, Bb and bb will be p2, 2pq and q2

These frequencies sum to one; hence:
p2 + 2pq + q2 = 1

89
Q

What is DNA sequencing?

A

The process of determining the precise order of nucleotides within a segment of a gene on a chromosome
It includes any method or technology used to determine the specific order of adenine, guaninine, cytosine and thymine in a strand of DNA

90
Q

What is DNA profiling?

A

Involves the testing of highly variable regions of an individual’s DNA that contain short repeating sequences called STRs located in the introns
-The exact number of STRs varies from person to person because we inherit DNA from our parents, DNA profiles can be used to confirm how closely related individuals are, trace inheritance patterns, and solve crimes

91
Q

What is PCR?

A

PCR is a technique used to exponentially amplify large numbers of copies of a specific sequence of DNA
-The central paradigm to PCR is understanding that: All life contains genetic material that is unique to a species and individuals within a species contain unique genetic sequences
-As a result, PCR amplifies unique DNA sequences of any organism that can be shared to other nucleotide segments of a known source
-Used for genetic testing, medical diagnosis, DNA fingerprinting

92
Q

Genetic sequencing using PCR and electrophoresis

A

-Genetic sequencing is the process by which the exact sequence of nucleotides in a chromosome or gene is determined
-Special versions of the four nucleotides called chain-terminating, nucleotides are each labelled with a different colored fluorescent marker
-During PCR cycles different length DNA fragments will form due to the chain-terminating nucleotides stopping further sequencing.

93
Q

What is convervation genetics?

A

-The application of genetics to preserve species through maintaining variation within populations so that they are capable of coping with environmental change
-Scientists analyze alleles of multiple genes to examine the genetic diversity within a species

94
Q

What is an MVP (minimum viable population)?

A

-The ecological threshold that indicates the smallest number of individuals in a species or population capable of surviving in the wild for an ongoing amount of time
-Small populations are more susceptible to genetic drift than large populations
-This occurs when differential breeding leads to the loss of alleles from the gene pool due to the small number of individuals carrying particular alleles

95
Q

MVP example- African cheetahs

A

Cheetahs have very low levels of genetic variation
This is due to two significant population bottlenecks: the last ice age and 1800s onwards
Pre-Genetic screening: Captive breeding programs had high mortality rates
All modern cheetahs descended from a bottleneck

96
Q

What is the human genome project?

A

-An international effort to decode the entire sequence of the human genome
-A complete and accurate sequence of 3 billion DNA base pairs- an estimate 20,000 to 25000 genes have been identified

97
Q

DNA hybridisation

A

-A technique that separates the double stranded DNA of two species into single strands
-The single strands form each species are mixed and allowed to bind
-They are then reheated and the temperature of separation is recorded
-The more closely related the species, the stronger the hybridization, the higher the temperature required to separate the hybrid DNA= a more recent ancestor
-African chimpanzees and humans

98
Q

Cytochrome C

A

Cytochrome C is a highly conserved mitochondrial protein found in all eukaryotic organisms. It is involved in energy production (ATP)
-International mass spectrometry studies have been used to determine the amino acid sequence of Cytochrome C across multiple species
-IN more than thirty species 37 of the 104 amino acids are conserved (identical at that position)

99
Q

Comparative genomics

A

-Comparative genomics uses computer based analysis to compare the genome sequences of different species
-Results are used to study evolutionary relationships, conserved DNA sequences which indicate genes that are essential to life
-Genomic similarities and differences provide scientists with a better understanding of how the appearance and behavior of organisms has changed over time.