Module 5 Flashcards

1
Q

How much of all agricultural commodities are lost each year and why?

A
  • 10 - 20 %
  • Weeds, insects, microbes, rodents, birds
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2
Q

What does food deterioration involve?

A
  • Heat, cold, light, oxygen, moisture, dryness, food enzymes, microorganisms, and macroorganisms.
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3
Q

What are the 3 general categories of microorganisms?

A
  • The good, the bad, and the ugly
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4
Q

Which microorganisms grow fastest in foods?

A

Bacteria

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5
Q

Bacteria may exist in two forms, which are:

A
  • Vegetative cells - actively metabolizing cells
  • Spores - dormant form
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6
Q

Yeasts grow more slowly than bacteria but […].

A
  • can tolerate more severe environmental conditions, for instance:
    • not inhibited by pH to the same extent
    • can grow in low water activity foods
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7
Q

Describe moulds.

A
  • Filamentous, produce spores
  • Can grow on foods that have low pH and lower water activities
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8
Q

Describe oxygen requirements of microbes.

A
  • Aerobic - grow only in presence of oxygen
  • Anaerobic - grow only in the absence of oxygen
  • Facultative anaerobic - can grow in the presence or absence of oxygen
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9
Q

What are psychrophiles or psychrotrophs?

A
  • Grow well in cold temperatures
  • Psychrotrophs are the major cause of spoilage in refrigerated foods.
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10
Q

What are mesophiles?

A
  • Grow well in moderate temperatures.
  • Most spoilage and disease-causing organisms are mesophiles.
  • Most have optimum growth temperature of 37C.
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11
Q

What are thermophiles?

A
  • Grow best at high temperatures
  • Most are spoilage causing
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12
Q

Pests are major contributors to post-harvest losses. Rodents alone ruin […].

A

> 30,000,000 tonnes of food each year worldwide

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13
Q

Give examples of enzymes endogenous to foods.

A
  • The softening of fruit tissue and the changes in flavour and colour during storage after picking are examples of deterioration of food quality by enzymes. It is enzymatic action that causes the softening of apple tissue during storage, with texture changing from that of a crisp apple to a somewhat soft and mealy apple with a concomitant loss of sweetness. Enzymes in the apple tissue degrade pectins that cement the cells together, leading to textural change.
  • You also no doubt have experienced the browning of apples, potatoes and peaches after they have been sliced and exposed to oxygen in the atmosphere. This browning phenomenon is known as enzymatic browning and is catalyzed by an enzyme known as polyphenol oxidase which catalyzes the oxidation of colorless phenols in the tissues to brown colored compounds, as shown in the following equation:
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14
Q

How can enzymes be inactivated? [3]

A
  • Heat
  • Chemicals (e.g., antioxidants)
  • Controlling the gaseous environment
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15
Q

Describe the temperature effects on microbial growth and enzymatic activity.

A
  • Rates of reactions generally increase as the temperature increases until an optimum is achieved, after which any further increase in temperature, causes the rates of reaction to decrease because of the inactivation of microorganisms or enzymes or because of inhibitory effects on chemical reactions.
  • Excessive heat also denatures proteins, breaks emulsions, removes moisture from foods (drying out), and destroys vitamins.
  • Cold temperatures can also deteriorate food. A well-known example is “chill injury”, the change in texture and discolouration of fruits and vegetables when they are exposed to freezing temperatures.
  • Freezing temperatures can deteriorate liquid foods such as milk, causing emulsions to break and fat to separate, and denaturing protein causing it to curdle or coagulate. “Freezer burn” due to loss of moisture can occur in solid and liquid foods.
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16
Q

Describe the effects of moisture on microbial and enzymatic activity.

A
  • Water loss during storage (e.g., wilting of lettuce in the refrigerator), or water uptake (e.g., by dehydrated foods) can lead to deterioration.
  • Retrogradation of starch, resulting in staling of bread, is caused by packing of linear starch molecules leading to the exclusion of water that was previously absorbed during gelatinization. The bread becomes tough and develops a dry texture.
  • Changes in water activity (free versus bound water) can influence chemical and enzymatic reactions and microbial growth.
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17
Q

Describe the effects of oxygen food quality.

A
  • Oxygen is an important factor in food quality since many oxidative reactions lead to deterioration in the quality of food and, in some cases, to losses in nutritive value. Oxidative deterioration is often accelerated by light.
  • For example, deterioration frequently occurs because of lipid oxidation in food products. The development of rancidity in breakfast cereals, vegetable oils and oil-based products, and deep-fried foods is due to the reaction of oxygen with fats, particularly those with high unsaturated fatty acid content. This type of rancidity is known as oxidative rancidity. This is in contrast to rancidity induced in foods upon the release of free fatty acids by very high temperatures or by the action of lipase enzymes, either endogenous or produced by spoilage-causing microorganisms. The latter rancidity is known as hydrolytic or lipolytic rancidity.
  • Oxidation of vitamins and colour pigments can lead to the deterioration of nutritive quality and aesthetic appeal of foods.
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18
Q

Describe the effects of physical deterioration on microbial and enzymatic activity.

A
  • Physical abuse causes tissue disruption and release of enzymes into tissues which can lead to changes such as enzymatic browning mentioned earlier.
  • Furthermore, improper packaging, for example, can cause crushing and tissue damage, making foods such as fruits and vegetables particularly susceptible to microbial invasion as well as enzymatic and chemical reactions.
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19
Q

Describe the effect of time on food quality.

A
  • For the majority of foods, quality will decrease with time.
  • Food preservation, packaging and storage practices are aimed to maintain this quality for as long as possible (shelf life); however, eventually the quality of any food will decrease with time.
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20
Q

What are perishable foods?

A
  • Foods that are not processed or are only minimally processed and have a shelf-life of 60 days or less
  • Spoilage is usually caused by microbial growth or senescence
  • E.g., meat, leafy vegetables, soft fruits, milk
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21
Q

What are semi-perishable foods?

A
  • Last between 2 to 6 months as a result of some form of preservation
  • E.g., ice cream, cheeses, dry snack foods
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22
Q

What are shelf-stable foods?

A
  • Shelf-life > 6 months
  • E.g., cereal grains, dehydrated pasta, some frozen, canned foods, and dehydrated vegetables
23
Q

How are microorganisms controlled?

A
  • Keeping them out of food
  • Removing them
  • Delaying initiation of growth
  • Killing them and their spores
24
Q

What are the preservation methods used to control microbes?

A
  • High temperature
  • Low temperature
  • Lowering water activity
  • Acids
  • Sugar and salt
  • Oxygen
  • Fermentation
  • Chemicals
25
Q

How is high temperature used to control microorganisms?

A
  • Thermal processing involves the application of heat to inactivate enzymes and destroy microorganisms.
  • Most bacteria are killed in the range of 82-93°C.
  • Spores are NOT destroyed even by boiling water at 100°C for 30 min.
  • To ensure sterility (total microbial destruction, including spores) a temperature of 121°C must be maintained for 15 minutes or longer.
  • There are various degrees of thermal processing:
    • blanching
    • pasteurization
    • commercial sterilization
26
Q

How are low temperatures used to control microorganisms?

A
  • Lowering the temperature of food decreases the rate of enzymatic, chemical and microbial reactions in food thus extending storage life.
  • Microbial growth rates decrease as temperatures decrease towards 0°C. Low temperatures, however, favour the proliferation of psychrotrophic microorganisms which ultimately cause spoilage of cold-stored foods.
  • There are two main categories of low-temperature storage of food:
    • refrigeration
    • freezing
  • Microorganisms are not easily killed by frozen storage of foods although death will occur slowly. Consequently, freezing cannot be relied upon to rid food of microbial contamination (Bacillus species and Clostridium species are virtually unaffected by low temperatures).
  • Some microorganisms can grow at temperatures as low as -9.5°C. Thus when food is held at improper frozen storage temperatures, microbial growth and spoilage can still occur, especially after thawing.
27
Q

How can water activity be lowered?

A
  • Freezing water as crystals of pure water.
  • Physical removal of water (dehydration)
  • Removal of some of the water (concentration) by addition of substances that bind water in food (e.g., salt, sugar)
28
Q

Discuss water activity ranges of microbes.

A
  • Each specific organism has its specific range of Aw in which it will grow.
  • Bacteria normally need Aw of 0.90 and higher, yeast need >0.70, while moulds need 0.60-0.70 and higher.
  • Of course, there are always some exceptions. For example, the pathogenic bacteria Staphylococcus aureus can grow at Aw as low as 0.83-0.84, while the yeast Saccharomyces cerevisiae requires Aw of 0.90.
29
Q

How are acids used to control microorganisms?

A
  • pH 4.6 is a critical value in terms of microbial growth and stability.
30
Q

How are sugar and salt used to control microbes?

A
  • Decrease water activity
  • Dehydrating effect on microbial cells
31
Q

How is oxygen used to control microbes?

A

It is the oxygen in the air or within the food that determines whether food can support the growth of aerobic or anaerobic microorganisms.

  • Thus moulds can be inhibited from growing on foods by excluding oxygen. This is the function of waxes applied to rounds of cheese during aging and also is the function of paraffin wax placed on top of jams and jellies.
  • However, the removal of oxygen from low acid foods with a high water activity can pose a potential health hazard because conditions can be created whereby anaerobic disease-causing microorganisms, such as Clostridium botulinum, may be able to proliferate and produce toxins that could cause disease when the toxin-containing food is consumed.
  • Vegetables and fish can be safely stored in an oxygen-free environment only if the Clostridium botulinum spores are killed by the application of heat.
32
Q

How is fermentation used to control microbes?

A
  • Specific microorganisms (starter cultures) are cultured in certain foods to facilitate chemical changes in the foods such that the foods have a longer storage life.
  • Inhibitors such as acids, alcohol and bacteriocins (antimicrobial agents) are produced by the starter cultures. The inhibitors delay or prevent the growth of undesirable microorganisms.
33
Q

How are chemicals used to control microbes?

A
  • Antimicrobial agents
  • Antioxidants
34
Q

How are enzymes controlled in foods? [5]

A
  • (1) Heat, (2) acid, (3) exclusion of oxygen, (4) chemical reducing agents, or:
  • (5) Various forms of radiation (energy) can be used to preserve food.
    • Ionizing radiation or “food irradiation” can be used to inactivate microorganisms in food, and to destroy storage pests (insects, mites, flies), thereby extending the storage life of the food.
    • Microwave treatment of food can be used to inactivate enzymes and microorganisms through the generation of high temperatures as a result of the interaction of microwave energy with water in the food.
    • Infrared radiation is used to toast foods, keep foods hot and cook foods.
    • Ultraviolet energy is used to sterilize air and water used in food processing, particularly in the beverage industry.
35
Q

Dormant form of microorganisms.

A

Spores

36
Q

Active form of microorganism.

A

Vegetative

37
Q

Spores transitioning to active form

A

Germinating

38
Q

Low heat denatures enzymes and can destroy microbes.
True or False?

A

False.

39
Q

Excessive heat denatures enzymes and can destroy microbes
True or False?

A

True

40
Q

Excessive heat denatures enzymes but promotes microbial growth.
True or False?

A

False

41
Q

Low heat denatures enzymes but promotes microbial growth.
True or False?

A

False.

42
Q

By controlling all aspects of food spoilage we can guarantee indefinite shelf life.
True or False?

A

False

43
Q

Changes in free water influences enzymatic, microbiological and chemical spoilage.
True or False?

A

True.

44
Q

Changes in free water influences enzymatic and microbiological spoilage and not chemical.
True or False?

A

False.

45
Q

Reducing water activity has minimal effect on the prevention of spoilage
True or False?

A

False.

46
Q

Enzymes present in microorganisms only cause food spoilage as none are present in food items like vegetables or meats.
True or False?

A

False

47
Q

Enzymes are found naturally in all living things including plants and microorganisms.
True or False?

A

True

48
Q

Enzymes are found in plants like vegetables and in meats but not in microorganisms like bacteria.
True or False?

A

False.

49
Q

Both enzymes and microorganisms are considered living things.
True or False?

A

False.

50
Q

Enzymes are […] which are one of the major food components. Enzymes are considered biological […] because they assist chemical reactions to occur.

A

Enzymes are proteins which are one of the major food components. Enzymes are considered biological catalysts because they assist chemical reactions to occur.

51
Q

Will grow in the presence of dissolved oxygen

A

Aerobic or facultative anaerobic

52
Q

Will not grow in the presence of dissolved oxygen

A

Anaerobic

53
Q

Can cause the spoilage of foods in the refrigerator

A

Psychrophiles

54
Q

Spoilage causing organisms that grow at high temperatures above 37C.

A

Thermophiles