MODULE 2 BIOSAFETY Flashcards
T OR F U CAN USE EXCESSIVE FORCE WHEN COLLECTING SAMPLES
F. DO NOT
T OR F SOFT PALATE IS NOT THE MOST SENSITIVE PART OF THE PALATE
FALSE. IT IS THE MOST
PPE must be ____ and ______
water resistent and back enclsure
PPE must be ____ and ______
water resistent and back enclsure
PPE must be ____ and ______
water resistent and back enclsure
Both NPS andOPS must be placed in UTM tube for what reason?
to increase antigenic load
VIruses are ______
thermolabile
T or F all potentially infectious substances must properly dientified and shipping classification
True
Screw capped container is and example of what type of container?
Secondary container
Box container is an example of what type container ?
Outer
how to transport infectious spx for international testing?
outer container must contain dry ice
process of cleansing an object or subtnce to remove contaminants
decontamination
primary objective ng decontamination
to reduce the level of contamination in lab
this term is less lethal than sterilization
disinfection
factors AFFECTING THE EFFECTIVENESS OF DISINFECTION PROCEDURE
nature and number of icrooganisms, amount of organic matter, type and condition of instruments
a process where all microorganisms are completely eliminated
Sterilization
this method is the most dependable system available for decontamination
autoclave
characteristics of a good autclave
Temp-121 C, pressure 15 psi, time is based on the requirements
2 types of autoclave
gravity (conventional), Vaccum assisted
This tyoe of autoclave is the most common
conventional
Ways to monitor autoclaave performace
- chemical (time, pressure etc
- Biological indicator (using geobacillus stearothemophilus) done weekly
The most protective hierarchy control
elimination or subtitution
The least protective hierarchy control
ppe
This containment level contains the AGENT AT THE SOURCE
Primary containment
This containment level was used as protection when there is a release from primary containment
Secondary containment
fume hood laminar flor bench and class 2 type are example os
Ventilated enclosures
deep cleaning of biosafety cabinet must be done ______
monthly
The operator should not be trained in the use of bsc t or f
false , must be trained
the bsc should be certified at least _____
once a year
t or f germicidal uv lamp can be good subtitutes for proper cleaning of bsc work zone
false q
the most important concept of biosafety and needs to be strictly adhered to
Standard microbiological practices
BSC and PPE are example of what type of Barriers?
Primary Barriers
Laboratories musr have sink for handwashing located at ______
near next door
lab chairs must be covered with material that are _____ to prevent water or liquids to pass through the chair
nonporous
Vacuum line must have —- INSIDE IT
HEPA FILTERS