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Biosafety > MODULE 1 BIOSAFETY TRAINING > Flashcards

MODULE 1 BIOSAFETY TRAINING Flashcards

1
Q

important to wash hands ______ and _______ working in the lab

A

before and after

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2
Q

This practice is the gold standard in handwashing

A

Hand rubbbing

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3
Q

The use of this device is to detect the effectiveness of handwashing

A

UV LIGHT

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4
Q

This is the most effective hand decontamination method

A

Handwashing

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5
Q

This the quickest method for hand decontamination method

A

Hand rubbing

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6
Q

Hierarachy of Controls where personnel change the way people work

A

Administrative controls

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7
Q

Hierarchy of controls where people are isiolated from the hazard

A

Engineering controls

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8
Q

A specialized clothing or equipment worn by an employee for protection for protection against infectrious materials

A

PPE

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9
Q

XAMPLES OF ROUTES OF EXPOSURE

A

INGESTION, CONTACT, PERCUTANEOUS, INHALATION

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10
Q

t or f: gloves must not be selected for the specific activity and hazard concerned

A

F

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11
Q

t or f : always work from “dirty to clean”

A

false, must be “clean to dirty”

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12
Q

TYPE OF REPIRATOR: remove contaminants by passing the breating air thourgh a purfying element

A

APR

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13
Q

n95, p100, papr are example of what type of respirator?

A

APR

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14
Q

SCBA is an example pf what type of respirator?

A

Air- supplying respirators

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15
Q

Fit test evaluates what?

A

evaluates the existence of leaks

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16
Q

Gene Expert cartridges must be done in what BSL ?

A

BSL 2

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17
Q

What are the other core requirements in Biosafety Guideline in COvid 19 lab?

A

Directional, airflow, negative air pressure room, SPecific training, Body temp, use of additional ppe

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18
Q

The recommended number of samples to be tested in 8 hr shift must not exceed at how many samples a day?

A

must not exceed 28 samples

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19
Q

Gloves are changed after processing the samples and _____ loading the cartridge inside the machine

A

Before

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20
Q

_____ and _____ are recommended disinfectants for decontaminating work surfaces and requipments

A

Ethanol and na hypochlorite

21
Q

How to dispose used cartridges for gene xpert?

A

Soak the cartrdige in 1:10 dilution of Na hypochlorite solution for 30 mins and properly labelled as hazardous waste

22
Q

Used swabs must be soaked in ___________ in ____ minutes

A

3-5% lysol solution in 30 mins before autoclaving

23
Q

For general surface decontamination, use _______ at least 10-15 mins contact time

A

1:10 bleach diluted

24
Q

Primary containers that contains specimen must not be leak proof t or f

A

f

25
Q

What are the storage requirements to the following equipments :
1. Refrigerator
2.Medical Freezer
3. Ultra ow freezer

A
  1. 2-8 C
  2. -20- 30 C
  3. -70 - 80 C
26
Q

Respiratory samples (e.g. Nasopharyngeal Aspirate / Oropharyngeal Aspirate) must be
placed directly in a _____ storage at _____ temp

A

Cold, Ref temp

27
Q

biopsy specimens must be fixed t or f

A

false, it must be fresh

28
Q

biopsy spx are transported in container with _____

A

ice

29
Q

Fresh blood, stool and urine samples must be processed at once at _______ upon receipt.

A

ambient room temp upon receipt

30
Q

Informed consent is NOT required if specimen of any type will be used for research purposes. t or f

A

false, must be required

31
Q

After splitting the spx into 2 aliqouts, Aliqout 1 is used for _______ while aliqout 2 is used for _______

A

RNA extraction , Fresh specimen

32
Q

How many months are these spx are stored for future use?

  1. respiratory swabs
  2. Blood serum, plasma
A
  1. 3 months
  2. 7 days
33
Q

t or f All positive samples are retained.

A

true

34
Q

Samples for Indefinite Storage / Retention, Both the negative and positive samples could be retained based on
institutional policy and guidelines. t or f

A

true

35
Q

In case that the facility has no capacity to store the samples, retention samples
can be transferred to the National Reference Laboratory of RITM, banked to
a biological repository or destroyed at least ___ after collection in
accordance with institutional policies and guidelines.

A

3 months

36
Q

This written material must be established to document allowable uses of covid 29 spx

A

Material Transfer Agreement

37
Q

COVID-19 original specimen aliquot must be shipped as Category __

A

Category B

38
Q

COVID-19 viral culture, isolates or viral concentrates must be shipped
as Category __

A

Category A

39
Q

_______ is the process of reviewing and eliminating selected specimens by destruction.

A

Culling

40
Q

Selected negative samples for destruction shall be autoclaved at ______________

A

121°C, 15 psi for 30 minutes
prior to disposal.

41
Q

Engineering controls includes what ?

A

Air handling, BSC, Lab access, FAciloty

42
Q

Risk Assesment, SOPS, emeergency response are exampls of what type of Control?

A

Administrative control

43
Q

in Air handling, the air must flow form an area from _______ contamination to ____ contamination. This must be checked _______

A

Lower to higher , daily

44
Q

HEPA filters, are capable of filtering paper greater 99.7 % of airborne particles smaller or larger than 0.3 uum in diameter. t or f

A

t

45
Q

Na hypochlorite is corrosive t or f

A

t

46
Q

___________ is a systematic process of gathering
information and evaluating the likelihood and impact of
exposure to or release of workplace hazard(s), and determining
the appropriate risk control measures to reduce the risk to an
acceptable level.

A

Risk assesement

47
Q

In centrifugation of spx during covid19 testing, the tube must always be ______

A

sealed.

48
Q

serves as the backbone of the BRM

A

Biorisk asssement

Biosafety (2 decks)

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