MODULE 1 BIOSAFETY TRAINING Flashcards
important to wash hands ______ and _______ working in the lab
before and after
This practice is the gold standard in handwashing
Hand rubbbing
The use of this device is to detect the effectiveness of handwashing
UV LIGHT
This is the most effective hand decontamination method
Handwashing
This the quickest method for hand decontamination method
Hand rubbing
Hierarachy of Controls where personnel change the way people work
Administrative controls
Hierarchy of controls where people are isiolated from the hazard
Engineering controls
A specialized clothing or equipment worn by an employee for protection for protection against infectrious materials
PPE
XAMPLES OF ROUTES OF EXPOSURE
INGESTION, CONTACT, PERCUTANEOUS, INHALATION
t or f: gloves must not be selected for the specific activity and hazard concerned
F
t or f : always work from “dirty to clean”
false, must be “clean to dirty”
TYPE OF REPIRATOR: remove contaminants by passing the breating air thourgh a purfying element
APR
n95, p100, papr are example of what type of respirator?
APR
SCBA is an example pf what type of respirator?
Air- supplying respirators
Fit test evaluates what?
evaluates the existence of leaks
Gene Expert cartridges must be done in what BSL ?
BSL 2
What are the other core requirements in Biosafety Guideline in COvid 19 lab?
Directional, airflow, negative air pressure room, SPecific training, Body temp, use of additional ppe
The recommended number of samples to be tested in 8 hr shift must not exceed at how many samples a day?
must not exceed 28 samples
Gloves are changed after processing the samples and _____ loading the cartridge inside the machine
Before
_____ and _____ are recommended disinfectants for decontaminating work surfaces and requipments
Ethanol and na hypochlorite
How to dispose used cartridges for gene xpert?
Soak the cartrdige in 1:10 dilution of Na hypochlorite solution for 30 mins and properly labelled as hazardous waste
Used swabs must be soaked in ___________ in ____ minutes
3-5% lysol solution in 30 mins before autoclaving
For general surface decontamination, use _______ at least 10-15 mins contact time
1:10 bleach diluted
Primary containers that contains specimen must not be leak proof t or f
f
What are the storage requirements to the following equipments :
1. Refrigerator
2.Medical Freezer
3. Ultra ow freezer
- 2-8 C
- -20- 30 C
- -70 - 80 C
Respiratory samples (e.g. Nasopharyngeal Aspirate / Oropharyngeal Aspirate) must be
placed directly in a _____ storage at _____ temp
Cold, Ref temp
biopsy specimens must be fixed t or f
false, it must be fresh
biopsy spx are transported in container with _____
ice
Fresh blood, stool and urine samples must be processed at once at _______ upon receipt.
ambient room temp upon receipt
Informed consent is NOT required if specimen of any type will be used for research purposes. t or f
false, must be required
After splitting the spx into 2 aliqouts, Aliqout 1 is used for _______ while aliqout 2 is used for _______
RNA extraction , Fresh specimen
How many months are these spx are stored for future use?
- respiratory swabs
- Blood serum, plasma
- 3 months
- 7 days
t or f All positive samples are retained.
true
Samples for Indefinite Storage / Retention, Both the negative and positive samples could be retained based on
institutional policy and guidelines. t or f
true
In case that the facility has no capacity to store the samples, retention samples
can be transferred to the National Reference Laboratory of RITM, banked to
a biological repository or destroyed at least ___ after collection in
accordance with institutional policies and guidelines.
3 months
This written material must be established to document allowable uses of covid 29 spx
Material Transfer Agreement
COVID-19 original specimen aliquot must be shipped as Category __
Category B
COVID-19 viral culture, isolates or viral concentrates must be shipped
as Category __
Category A
_______ is the process of reviewing and eliminating selected specimens by destruction.
Culling
Selected negative samples for destruction shall be autoclaved at ______________
121°C, 15 psi for 30 minutes
prior to disposal.
Engineering controls includes what ?
Air handling, BSC, Lab access, FAciloty
Risk Assesment, SOPS, emeergency response are exampls of what type of Control?
Administrative control
in Air handling, the air must flow form an area from _______ contamination to ____ contamination. This must be checked _______
Lower to higher , daily
HEPA filters, are capable of filtering paper greater 99.7 % of airborne particles smaller or larger than 0.3 uum in diameter. t or f
t
Na hypochlorite is corrosive t or f
t
___________ is a systematic process of gathering
information and evaluating the likelihood and impact of
exposure to or release of workplace hazard(s), and determining
the appropriate risk control measures to reduce the risk to an
acceptable level.
Risk assesement
In centrifugation of spx during covid19 testing, the tube must always be ______
sealed.
serves as the backbone of the BRM
Biorisk asssement
