MODULE 1 BIOSAFETY TRAINING

Question 1

important to wash hands ______ and _______ working in the lab

Answer 1

before and after

Question 2

This practice is the gold standard in handwashing

Answer 2

Hand rubbbing

Question 3

The use of this device is to detect the effectiveness of handwashing

Answer 3

UV LIGHT

Question 4

This is the most effective hand decontamination method

Answer 4

Handwashing

Question 5

This the quickest method for hand decontamination method

Answer 5

Hand rubbing

Question 6

Hierarachy of Controls where personnel change the way people work

Answer 6

Administrative controls

Question 7

Hierarchy of controls where people are isiolated from the hazard

Answer 7

Engineering controls

Question 8

A specialized clothing or equipment worn by an employee for protection for protection against infectrious materials

Answer 8

PPE

Question 9

XAMPLES OF ROUTES OF EXPOSURE

Answer 9

INGESTION, CONTACT, PERCUTANEOUS, INHALATION

Question 10

t or f: gloves must not be selected for the specific activity and hazard concerned

Answer 10

F

Question 11

t or f : always work from “dirty to clean”

Answer 11

false, must be “clean to dirty”

Question 12

TYPE OF REPIRATOR: remove contaminants by passing the breating air thourgh a purfying element

Answer 12

APR

Question 13

n95, p100, papr are example of what type of respirator?

Answer 13

APR

Question 14

SCBA is an example pf what type of respirator?

Answer 14

Air- supplying respirators

Question 15

Fit test evaluates what?

Answer 15

evaluates the existence of leaks

Question 16

Gene Expert cartridges must be done in what BSL ?

Answer 16

BSL 2

Question 17

What are the other core requirements in Biosafety Guideline in COvid 19 lab?

Answer 17

Directional, airflow, negative air pressure room, SPecific training, Body temp, use of additional ppe

Question 18

The recommended number of samples to be tested in 8 hr shift must not exceed at how many samples a day?

Answer 18

must not exceed 28 samples

Question 19

Gloves are changed after processing the samples and _____ loading the cartridge inside the machine

Answer 19

Before

Question 20

_____ and _____ are recommended disinfectants for decontaminating work surfaces and requipments

Answer 20

Ethanol and na hypochlorite

Question 21

How to dispose used cartridges for gene xpert?

Answer 21

Soak the cartrdige in 1:10 dilution of Na hypochlorite solution for 30 mins and properly labelled as hazardous waste

Question 22

Used swabs must be soaked in ___________ in ____ minutes

Answer 22

3-5% lysol solution in 30 mins before autoclaving

Question 23

For general surface decontamination, use _______ at least 10-15 mins contact time

Answer 23

1:10 bleach diluted

Question 24

Primary containers that contains specimen must not be leak proof t or f

Answer 24

f

Question 25

What are the storage requirements to the following equipments : 1. Refrigerator 2.Medical Freezer 3. Ultra ow freezer

Answer 25

1. 2-8 C 2. -20- 30 C 3. -70 - 80 C

Question 26

Respiratory samples (e.g. Nasopharyngeal Aspirate / Oropharyngeal Aspirate) must be placed directly in a _____ storage at _____ temp

Answer 26

Cold, Ref temp

Question 27

biopsy specimens must be fixed t or f

Answer 27

false, it must be fresh

Question 28

biopsy spx are transported in container with _____

Answer 28

ice

Question 29

Fresh blood, stool and urine samples must be processed at once at _______ upon receipt.

Answer 29

ambient room temp upon receipt

Question 30

Informed consent is NOT required if specimen of any type will be used for research purposes. t or f

Answer 30

false, must be required

Question 31

After splitting the spx into 2 aliqouts, Aliqout 1 is used for _______ while aliqout 2 is used for _______

Answer 31

RNA extraction , Fresh specimen

Question 32

How many months are these spx are stored for future use? 1. respiratory swabs 2. Blood serum, plasma

Answer 32

1. 3 months 2. 7 days

Question 33

t or f All positive samples are retained.

Answer 33

true

Question 34

Samples for Indefinite Storage / Retention, Both the negative and positive samples could be retained based on institutional policy and guidelines. t or f

Answer 34

true

Question 35

In case that the facility has no capacity to store the samples, retention samples can be transferred to the National Reference Laboratory of RITM, banked to a biological repository or destroyed at least ___ after collection in accordance with institutional policies and guidelines.

Answer 35

3 months

Question 36

This written material must be established to document allowable uses of covid 29 spx

Answer 36

Material Transfer Agreement

Question 37

COVID-19 original specimen aliquot must be shipped as Category __

Answer 37

Category B

Question 38

COVID-19 viral culture, isolates or viral concentrates must be shipped as Category __

Answer 38

Category A

Question 39

_______ is the process of reviewing and eliminating selected specimens by destruction.

Answer 39

Culling

Question 40

Selected negative samples for destruction shall be autoclaved at ______________

Answer 40

121°C, 15 psi for 30 minutes prior to disposal.

Question 41

Engineering controls includes what ?

Answer 41

Air handling, BSC, Lab access, FAciloty

Question 42

Risk Assesment, SOPS, emeergency response are exampls of what type of Control?

Answer 42

Administrative control

Question 43

in Air handling, the air must flow form an area from _______ contamination to ____ contamination. This must be checked _______

Answer 43

Lower to higher , daily

Question 44

HEPA filters, are capable of filtering paper greater 99.7 % of airborne particles smaller or larger than 0.3 uum in diameter. t or f

Answer 44

t

Question 45

Na hypochlorite is corrosive t or f

Answer 45

t

Question 46

___________ is a systematic process of gathering information and evaluating the likelihood and impact of exposure to or release of workplace hazard(s), and determining the appropriate risk control measures to reduce the risk to an acceptable level.

Answer 46

Risk assesement

Question 47

In centrifugation of spx during covid19 testing, the tube must always be ______

Answer 47

sealed.

Question 48

serves as the backbone of the BRM

Answer 48

Biorisk asssement