MODULE 2 Flashcards
Anabolic
small molecules assemble into large ones
* energy is required
Catabolic
large/complex molecules break down into smaller ones
* energy is released
Oil Rig
oxidation involves loss, reduction involves gain
NAD+/FAD involved in a lot of ____ reactions
catabolic
ATP abbrev.
adenosine triphosphate
ADP abbrev.
adenosine diphosphate
Heterotrophy
Organisms that eat other organisms or preformed organic matter
Autotrophy
Create their own food from inorganic nutrients
* Photosynthesis
Light reaction: cyclic photophosphorylation
Uses sunlight to make proton gradient
* Leads to formation of ATP
Evolutionary advantage over heterotrophs
* Werent dependant on depletable resources
Light reaction: non-cyclic photophosphorylation
Built on the earlier version
Uses sunlight to make ATP and NADPH
* Have everything they need to convert CO2 to organic compounds (glucose)
* Evolutionary advantage over cyclic photophosphorylation
Proton pumping
High proton in Lumen, low in stroma
Travel to stroma through ATP Synthase
Diffuse back through it
Catalysed the phosphorylation of Pi onto ADP -> ATP
Endosymbiotic origin of photosynthetic eukaryotes
Engulfed prokaryotes > developed symbioses > lived as individual organelles > eukaryotes
3 stages of calvin cycle
- Carboxylation
- Reduction
- Regeneration
Carboxylation
1st
Carboxylation of RuBP with CO2 pulled by Rubisco > 6C molecule is unstable > 2x 3-PGA
Regeneration (calvin)
2nd
ATP + NADPH used to change 3-PGA into G3P
Reduction
3rd
5/6 G3P stay in cycle > modified with ATP to make more RuBP
Photorespiration
Fixation of oxygen by rubisco
* 2-phosphoglycolate
* Detoxified by Chloraplast, Mitochondrion, Peroxisome
* ATP expended, CO2 released
C4
Adjustments exploiting PEP carboxylase to concentrate CO2 around Rubisco
CAM
Saves water
* stomata only open at night
* humidity is higher
* CO2 stored as C4 in vacuole
In what kind of plants do you tend to see CAM photosynthesis
Plants that live in dry climate
* E.g. cactus
Aerobic respiration
- Glycolysis
- Link reaction
- Krebs cycle
- Oxidative phosphorylation
where does respiration occur?
Mitochondria
1. Gly - outside
2. Kreb’s - matrix
3. ETC - inner membrane
Glycolysis
Sugar splitting
1. Energy investment
2. Energy payoff
Net output of Glycolysis
- 2 ATP
- 2 pyruvate + H2O
- 2 NADH + 2 protons
Krebs cycle
Remove electrons from products of glycolysis
* Pyruvate > Acetyl CoA
* small ATP produced
* 3 NADH + H+
* 1 FADH2
* 2 CO2
ETC
NADH -> NAD+ + H+ & FADH2 -> FAD + 2H+
* Pump protons into IM space
* High conc. Of H+ in IM space
* Low conc. in matrix
* Want to flow back
* Only way, through ATP synthase
* Produce ATP
Special adaptations to increase SA of inner membrane
- Thylakoids stacked into granum - in stroma
- Elaborate folding into crista - in matrix
AOX and UCP
Downregulate production of ATP when the plant already has enough
* AOX - bypass
* UCP - uncoupling protein, diffuse back
Composition of the Body
- 60% water
- 18% protein
- 16% fat
-
6% Carbohydrates, minerals etc. micro nutrients
Changes as animal gets older
Condensation/dehydration of carbs
CONDENSATION - ANA
HYDROLYSE - CATA
Glycogen is stored many places including…
- LIVER: breaks down carbs, releases sugar into bloodstream
- MUSCLES: trap sugar within individual muscles cells, do not share energy
What hormone causes catabolism of glycogen?
glucagon
When you eat food, your blood stream does not absorb protein…
Absorbs amino acids
* Food broken down to AA
* Absorbed in villus
* Brought to blood
Amino acid structure
Amino group (NH2)
Carboxyl group (COOH)
R group
Non essential amino acids
The cell can make it itself
Essential amino acids
Cannot be manufactured in body
* must be in food
All essential amino acid components of protein are made by…
Plants
* Every animal has to derive the essential amino acids from plants
* Carnivore eats herbivore who has eaten this amino acid
Fat is stored in ____ tissue
Adipose
* Omentum - large flat adipose tissue layer nestling on the surface of the intra-peritoneal organs
Is phosphate hydrophilic or hydrophobic?
5 bonds, 2 O dont have H attached
* Hydrophillic
Omentum role
- Protection
- Storage
- Release energy for metabolism at a later stage
Adenosine triphosphate
Stores a lot of energy from breaking food molecules
* Ribose sugar (5 carbon)
* Base adenine - rings with nitrogen
* 3 phosphate molecules attached
* - charge on oxygen
* When it loses P generates energy
Fats, proteins and carbohydrates are interconvertible?
True
Endocrine cells
Respond to homeostatic signals and release hormones
* Travel through bloodstream > affect physiology of cells.
Fibroblasts
Repair wounds
* Move to site of injury, lay down fibrin and close down wound
Prokaryotes
Single cell, no membrane bound nucleus
Bacteria
Peptidoglycan (sugar and amino acid lattice) major constituent of cell wall
Archaea
Do not have peptidoglycan as major constituent of cell wall, often live in extreme conditions
Protists
Single-celled eukaryotes
Animal plasma membranes are stabilised with _____
cholesterol
Cholesterol
- Hydrophillic (attarcted to phosphate heads)
- Hydrophobic (attracted to fatty acid tails)
- Side chain
Evolution of nucleus (theory)
Invagination
* Folds in (nuclear envelope)
* More pockets (ER)
Evolution of the mitochondria (theory)
Absorb aerobic prokaryotic cell
* Membrane derived from eukarotic cell
* Now has enzymes to break down pyruvic acid (energy)
Fibrous proteins
- Make up cytoskeleton - shape, hang rest of tissues on
- Pathways
Key differences in animal cell
No cell wall
No big vacuole
No chloroplasts
Key differences in plant cell
Cell wall
Chloroplasts
Huge central vacuole
The Plant Cell Wall
Made of cellulose
* Long strings, carbohydrate
* Good for structure
The endoplasmic reticulum (ER) is connected to the ____
Nucleus
* Nucleus has pores
Describe the flow of information from DNA to protein in the context of the cell.
Nucleus > DNA > transcription > mRNA > Ribosome > translation > protein.
Flagellum
Propels bacteria
* Harvest power of H ions, passing through channels in the apparatus
Vesicles carry protein cargo
Come from ribosomes in rough ER
* Travel into vesicles
* Travel into cis-face of golgi apparatus
* Disengage from trans face
* Exocytosis: Take to exterior of cell , fuse with membrane
* Endocytosis: proteins bind to surface of plasma membranes - receptors, folds in, draw into cell
When a protein is fully drawn into a cell
Endosome
What happens to protein that has entered the cell?
Fed to golgi or lysosome - break down
F0, F1 complexes
Mito
* take molecule of ADP and phosphate > ATP
Name 3 major biological functions of mitochondria
- Production of ATP (ATP synthesis)
- Steroid synthesis
- Apoptosis - packaged then eaten by immune system
Mitochondria are often attached to
Microtubules of the cytoskeleton
* Pathways
* Hold in place
* Actin & myosin
* Motor proteins
Motor proteins
- Portion attaches to mitochondria/vesicles
- Feet
3 components of the cytoskeleton
- Microfilaments - made of actin
- Microtubules - made of tubulin, train tracks
- Intermediate filaments - made of various proteins, strong, reinforcing, resist stress, often keratin.
Passive diffusion
Semi-permeable membrane
* molecules and charged molecules
Powered transport
Push molecules against a gradient
* ATP
* Phosphorylation event
* Top close off, bottom open up
Phagocytosis
Engulf bacterial cell
* Close over
Pinocytosis
Drinking
* Fluids along with dissolved molecules
Receptors in endocytosis
Specifically take in one type of molecule
* recognise 3D structure
* Proteins line up under membrane - help bud off
Most multicellular animals are made out of just 4 major tissue types
- Epithelium 🦶🏻
- Connective 🪢
- Nervous 🧠
- Muscle 💪🏼
Connective tissue
- Fibres
- Cells
-
Ground substance (proteins, water, slats, minerals, cells)
Varying densities
Blood - coagulate
Epithelial tissue
Covering on all internal and external surfaces of your body, lines body cavities and hollow organs
Muscle
- Cardiac
- Smooth - hollow organ
- Skeletal - voluntary, movement
Epithelia is anchored to the ____
Basal lamina
* Surround muscle and fat tissues
Desmosome
Mediate cell-cell contact and strong adhesion.
Hemidesmosome
One plaque on the inside portion of the cell
* Use connecting proteins to anchor down directly into basal lamina
Cancer originates in ____ tissue
Epithelial
* Basal lamina can hold back metastasis for a while
Nervous tissue
Coordinated movement and behaviour
Use ____ which signal cells to change their physiology
hormones
Steroid hormones
- Go straight through plasma membrane
- Receptor in cytoplasm/nucleus
- Produce a protein
Neurotransmitters
Binding to a receptor coupled to a channel
Protein hormones
- Interact with receptor outside of cell, in plasma membrane
- Interact with series of relay proteins
The stress response
- CRH enters bloodstream that connects hypothalamus to pituitary
- Stimulates ACTH > into bloodstream
- Reaches adrenal cortex, releases cortisol
- Goes into fat supplies > breakdown (fuel)
Plant cell gradients
Hyper - Plasmolysed
Iso - Flaccid
Hypo - Turgid (optimal)
A260:A280 values and purity
- 1.8 - 2 = pure DNA
- < 1.8 = protein contamination
- > 2 RNA contamination
A260:A230 values and purity
- > 1.8 = pure nucleic acid
- < 1.8 = organic compunds
The stress response is a ____ feedback loop
Negative
Steroids are hydro… and proteins are hydro…
- Phobic
- Phillic
Cytoplasmic reticulum
Hold lots of calcium
* Send message to release this to activate muscles
* Go to actin binding sites